| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,655 | A→G | 39.1% | T138T (ACT→ACC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,655 | 0 | A | G | 39.1% | 10.6 / 21.4 | 23 | T138T (ACT→ACC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (5/9); new base G (5/4); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCT > minE/1015593‑1015713 | ttCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTtata < 1:1956898/71‑1 (MQ=255) cttcttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAg > 1:306349/1‑68 (MQ=255) tcttTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGcc > 1:1308063/1‑71 (MQ=255) tcttTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGcc > 1:3695163/1‑71 (MQ=255) tcttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACAcc > 1:1078024/1‑71 (MQ=255) tgtaCATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGcct < 1:228787/66‑2 (MQ=255) cATATCCAGCGGTGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCa < 1:3592687/71‑1 (MQ=255) cATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCa < 1:1279535/71‑1 (MQ=255) atatCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAg < 1:762288/49‑1 (MQ=255) tatCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAgc < 1:3927639/71‑1 (MQ=255) tCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGcctt < 1:184665/61‑3 (MQ=255) gcgcGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTtc < 1:1202475/70‑1 (MQ=255) cgcaTTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAg < 1:3494254/46‑1 (MQ=255) gcgTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTtata < 1:1139577/44‑1 (MQ=255) tttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAg > 1:912376/1‑41 (MQ=255) tttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAg > 1:745678/1‑55 (MQ=255) gCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcaccacca > 1:3236232/1‑71 (MQ=255) gCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcaccac < 1:3645300/69‑1 (MQ=255) atAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCgg < 1:997455/70‑1 (MQ=255) cgcAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTtcatca > 1:649220/1‑57 (MQ=255) aTTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgcc < 1:472427/43‑1 (MQ=255) aTCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCt > 1:1774856/1‑71 (MQ=255) aTCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCt > 1:969383/1‑71 (MQ=255) aGTGTTATAGACACCGCGCGCCAGCGCCGCTtcatc > 1:3317241/1‑36 (MQ=255) | TTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCT > minE/1015593‑1015713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |