| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,182 | G→A | 12.7% | A57T (GCG→ACG) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,182 | 0 | G | A | 12.7% | 166.8 / 12.2 | 63 | A57T (GCG→ACG) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (27/28); new base A (4/4); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
TTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAACACAACCCCCACTCACCACA > minE/218113‑218250 | ttCAAGGCAGTGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTg < 1:1133043/70‑1 (MQ=255)ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTg < 1:3432835/70‑1 (MQ=255)ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGc < 1:2383706/71‑1 (MQ=255)ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGc < 1:1626290/71‑1 (MQ=255) tCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACg > 1:2276626/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTTGCGTCAGTCCTTGCgg > 1:2889748/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:3418360/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACgg > 1:2222874/1‑71 (MQ=255) aaGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACggg > 1:2057439/1‑71 (MQ=255) gCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAg > 1:1061903/1‑71 (MQ=255) cAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGc < 1:1069311/71‑1 (MQ=255) gggTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCgg < 1:1027957/71‑1 (MQ=255) ggTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGc < 1:1948180/61‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCt > 1:3535584/1‑71 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCt < 1:2674328/71‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCt < 1:1114153/71‑1 (MQ=255) tAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGTAGCGGGCtt > 1:1790473/1‑71 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCttt > 1:1243038/1‑71 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCtt < 1:3607905/70‑1 (MQ=255) aGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCtt < 1:2100372/69‑1 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg > 1:1700406/1‑71 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg > 1:114188/1‑71 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGa < 1:2466753/60‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgt > 1:3072249/1‑70 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtaa > 1:1832434/1‑70 (MQ=255) tAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGc > 1:560724/1‑58 (MQ=255) aaGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGTGGAGCGGGCTTTCgtag > 1:3456885/1‑69 (MQ=255) aaGATCCCAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCATACgtagt > 1:2704951/1‑70 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTg < 1:2649544/48‑1 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTaa > 1:2145273/1‑71 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTaa < 1:1763557/71‑1 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTc > 1:3837798/1‑63 (MQ=255) gATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGc < 1:219190/48‑1 (MQ=255) gCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTc > 1:2038019/1‑60 (MQ=255) cGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCgtaatc < 1:3786105/65‑4 (MQ=255) gAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATAccc < 1:2576615/70‑1 (MQ=255) aaaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgta > 1:2859295/1‑59 (MQ=255) ggcagcTATGGTTAGAAACTACCTGACGGCAGTCCTTGCGGGGAGCAGGCTTTCgtaa < 1:2911956/57‑2 (MQ=255) gcagcCATGGATAGAAACTATCTCGCGTCAGTCCTTGCggg < 1:3587545/41‑1 (MQ=255) gcagcCATGGACAGAAACTACCTGGCGTCAGTCCTTGCg < 1:167262/39‑1 (MQ=255) gcagcCATGGACAGAAACTACCTGGCGTCAGTCCTTGCg < 1:3156852/39‑1 (MQ=255) cagcCATGAACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGActct < 1:338180/71‑1 (MQ=255) agcCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtaat < 1:1388687/56‑3 (MQ=255) agcCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtaat < 1:2745131/56‑3 (MQ=255) ccATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtagta < 1:917290/55‑1 (MQ=255) aTGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGCGCGGGCTTTCGTAGTAAt > 1:2516777/1‑55 (MQ=255) aTGGACAGAAACTACCTGGCGTAAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCg > 1:2602766/1‑71 (MQ=255) tGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAg < 1:1398882/37‑1 (MQ=37) tGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg < 1:1676202/47‑1 (MQ=255) tGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTcccc > 1:904705/1‑69 (MQ=255) gACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCgg > 1:1173149/1‑38 (MQ=255) aaaCTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTc > 1:2100415/1‑65 (MQ=255) aaaCTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTaa < 1:2332396/70‑1 (MQ=255) aaCTACCTGGCGTCAGTCCTTACGGGGAGCGGGCttt < 1:1687960/37‑1 (MQ=255) aCTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGGCCTTAAaca > 1:856117/1‑71 (MQ=255) aCCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATAcccc < 1:2816994/47‑1 (MQ=255) aCCTGGCGTCAGTCCTTGCGGGGAGCGGGATTTCGTAGTaa > 1:1626310/1‑41 (MQ=255) ccTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAACACAAc < 1:2506768/71‑1 (MQ=255) cTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAACACaa < 1:3752986/69‑1 (MQ=255) cAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGAct > 1:3807805/1‑42 (MQ=255) aGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGActct < 1:1072645/43‑1 (MQ=255) ccTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGAct > 1:2784837/1‑38 (MQ=255) cTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCgc > 1:3336290/1‑34 (MQ=255) cTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAACACAACCCCCACTcaccac < 1:3050594/71‑1 (MQ=255) cTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAACACAACCCCCACTcacca < 1:676791/70‑1 (MQ=255) tGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCg > 1:3123493/1‑42 (MQ=255) gCGGGGAGCGGGCTTTCGTAGTAATACCCCGActc > 1:287213/1‑35 (MQ=255) gCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTcccc > 1:3231989/1‑40 (MQ=255) gCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTcc > 1:3090294/1‑44 (MQ=255) gCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAACACAACCCCCACTCACcaca < 1:1624581/69‑1 (MQ=255) | TTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAACACAACCCCCACTCACCACA > minE/218113‑218250 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |