Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,015,672 G→T 22.4% R133S (CGC→AGC) ‡ fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,015,6720GT22.4% 48.8 / 3.4 22R133S (CGC→AGC) ‡fumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base G (7/10);  new base T (3/2);  total (10/12)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCG  >  minE/1015607‑1015739
                                                                 |                                                                   
cATATCCAGCGGTGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCa                                                                <  1:3592687/71‑1 (MQ=255)
cATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCa                                                                <  1:1279535/71‑1 (MQ=255)
  tatCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAgc                                                              <  1:3927639/71‑1 (MQ=255)
           gcgcGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTtc                                                      <  1:1202475/70‑1 (MQ=255)
                 tttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAg                                                               >  1:745678/1‑55 (MQ=255)
                    gCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcaccacca                                            >  1:3236232/1‑71 (MQ=255)
                    gCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcaccac                                              <  1:3645300/69‑1 (MQ=255)
                        atAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCgg                                        <  1:997455/70‑1 (MQ=255)
                            cgcAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTtcatca                                                  >  1:649220/1‑57 (MQ=255)
                                 aTTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgcc                                                           <  1:472427/43‑1 (MQ=255)
                                    aTCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCt                            >  1:1774856/1‑71 (MQ=255)
                                    aTCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCt                            >  1:969383/1‑71 (MQ=255)
                                                aGTGTTATAGACACCGCGCGCCAGCGCCGCTtcatc                                                   >  1:3317241/1‑36 (MQ=255)
                                                 gtgtTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTAcc               <  1:3023536/71‑1 (MQ=255)
                                                   gtTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaa             >  1:3869561/1‑71 (MQ=255)
                                                    ttATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaa             <  1:3554671/70‑1 (MQ=255)
                                                      ataGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTc                                      <  1:3671896/43‑1 (MQ=255)
                                                        aGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATaca                                >  1:2230814/1‑47 (MQ=255)
                                                        aGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCtttt                    >  1:2434756/1‑59 (MQ=255)
                                                        aGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat         >  1:449900/1‑70 (MQ=255)
                                                          acacCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTCTTACCAACaata        <  1:612243/69‑1 (MQ=255)
                                                            acCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACca              <  1:670534/61‑1 (MQ=255)
                                                              cgcgcgCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcgcg  <  1:1651817/71‑1 (MQ=255)
                                                                 |                                                                   
CATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCG  >  minE/1015607‑1015739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: