Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,015,715 C→G 11.3% G118G (GGG→GGC fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,015,7150CG11.3% 140.0 / 7.9 53G118G (GGG→GGCfumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base C (24/23);  new base G (3/3);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

GTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATG  >  minE/1015656‑1015784
                                                           |                                                                     
gtgtTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTAcc                                                            <  1:3023536/71‑1 (MQ=255)
  gtTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaa                                                          >  1:3869561/1‑71 (MQ=255)
   ttATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaa                                                          <  1:3554671/70‑1 (MQ=255)
       aGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCtttt                                                                 >  1:2434756/1‑59 (MQ=255)
       aGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat                                                      >  1:449900/1‑70 (MQ=255)
         acacCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTCTTACCAACaata                                                     <  1:612243/69‑1 (MQ=255)
           acCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACca                                                           <  1:670534/61‑1 (MQ=255)
             cgcgcgCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcgcg                                               <  1:1651817/71‑1 (MQ=255)
                  gcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaataat                                                   >  1:3655628/1‑62 (MQ=255)
                  gcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaataat                                                   >  1:3707824/1‑62 (MQ=255)
                  gcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGcc                                          >  1:3082898/1‑71 (MQ=255)
                       cgccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt                                     >  1:3474463/1‑71 (MQ=255)
                       cgccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt                                     >  1:2402544/1‑71 (MQ=255)
                        gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTa                                      >  1:1417215/1‑69 (MQ=255)
                          cgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat                                                      <  1:701938/51‑1 (MQ=255)
                            cTTCATCACCCCCGCCGGTCCACACGCGCTGGCCTTTTTTAcc                                                            >  1:486934/1‑43 (MQ=19)
                            cTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa                                >  1:2896941/1‑71 (MQ=255)
                             ttCATCACCACCACCGGTCCATACACGCTGCCCtttttt                                                               >  1:1519266/1‑39 (MQ=255)
                              tcatcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGcc                                          <  1:1444709/59‑1 (MQ=255)
                               catcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTgg                                <  1:2032231/68‑2 (MQ=255)
                                atcaCCACCACCGGTCCATACACGCTGCCCtttttt                                                               <  1:1518907/36‑1 (MQ=255)
                                 tcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGt                           >  1:847111/1‑71 (MQ=255)
                                 tcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGt                           >  1:3633187/1‑71 (MQ=255)
                                   accaccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTg                         >  1:439802/1‑71 (MQ=255)
                                   accaccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTg                         <  1:2462578/71‑1 (MQ=255)
                                    ccaccaccGGTCCATACACGCTGCCCTTTTTTACcaa                                                          >  1:2020991/1‑37 (MQ=255)
                                    ccaccaccGGTCCATACACGCTGCCCATTATTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTg                         <  1:732587/70‑1 (MQ=255)
                                     caccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTgg                        >  1:224662/1‑70 (MQ=255)
                                       ccgccGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAg                     >  1:1350056/4‑71 (MQ=255)
                                           cGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACg                 >  1:290796/1‑71 (MQ=255)
                                            ggTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGc                >  1:3045087/1‑71 (MQ=255)
                                             gTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGGGCCGGTATCCTGACAgg                            <  1:1781090/58‑1 (MQ=255)
                                              tCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCt              <  1:33834/71‑1 (MQ=255)
                                              tCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCt              >  1:1415530/1‑71 (MQ=255)
                                              tCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCt              >  1:1480523/1‑71 (MQ=255)
                                                cATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGcc               <  1:949855/68‑1 (MQ=255)
                                                aaTACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCa                      >  1:3489735/2‑61 (MQ=255)
                                                 aTACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTc           >  1:3650727/1‑71 (MQ=255)
                                                 aTACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTc           <  1:1151863/71‑1 (MQ=255)
                                                  tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTa                                      <  1:196216/43‑1 (MQ=255)
                                                  tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATc                                    <  1:2073736/45‑1 (MQ=255)
                                                  tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCt                                  <  1:3177596/47‑1 (MQ=255)
                                                  tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa                                <  1:2229768/49‑1 (MQ=255)
                                                  tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAg                     <  1:921888/60‑1 (MQ=255)
                                                  tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCtt             <  1:2996289/68‑1 (MQ=255)
                                                   acgcGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTggc         >  1:1523251/4‑71 (MQ=255)
                                                   acacGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGtt                          <  1:2033882/54‑1 (MQ=255)
                                                   acacGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgc         >  1:599650/1‑71 (MQ=255)
                                                     gcGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAg                             <  1:3920308/48‑1 (MQ=25)
                                                       gCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACa                              <  1:3428337/46‑1 (MQ=255)
                                                       gCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACa                              <  1:568705/46‑1 (MQ=255)
                                                        cTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTa                                      <  1:3925324/37‑1 (MQ=255)
                                                          gCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTCGCAGAACGcc               >  1:2014258/1‑58 (MQ=255)
                                                           cccTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATg  <  1:2857399/70‑1 (MQ=255)
                                                           |                                                                     
GTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATG  >  minE/1015656‑1015784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: