| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 219,996 | C→T | 32.8% | G711D (GGT→GAT) | fadE ← | acyl coenzyme A dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 219,996 | 0 | C | T | 32.8% | 71.5 / 49.8 | 61 | G711D (GGT→GAT) | fadE | acyl coenzyme A dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (19/22); new base T (10/10); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01 | |||||||||||
GGCAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGC > minE/219926‑220061 | ggcAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGAc > 1:1995698/1‑71 (MQ=255) aaTCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACcgc < 1:644941/71‑1 (MQ=255) aaTCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACcg > 1:552899/1‑70 (MQ=255) aaTCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCGCGCGCGGCGTCAGGTACTGACcgc < 1:2722277/71‑1 (MQ=255) aTCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACcgc > 1:2547847/1‑70 (MQ=255) aTCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACcgc > 1:3456952/1‑70 (MQ=255) aTCACATCCACCAGCGCCTCTTCCAGAAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACcgc > 1:1097003/1‑70 (MQ=255) tCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGa > 1:722938/1‑71 (MQ=255) acaTCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGAc > 1:2979205/1‑70 (MQ=255) ccaccaGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATg > 1:717154/1‑71 (MQ=255) ccaccaGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATg > 1:1609063/1‑71 (MQ=255) ccaGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAAt < 1:39509/67‑1 (MQ=255) ccaGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCgg < 1:1160176/71‑1 (MQ=255) cgcCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAAc < 1:226224/71‑1 (MQ=255) gcCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACg < 1:2502034/71‑1 (MQ=255) ctctTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACggg < 1:1814882/71‑1 (MQ=255) ctctTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACgg < 1:144061/70‑1 (MQ=255) tctTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGt > 1:2511753/1‑71 (MQ=255) tctTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGt > 1:2137723/1‑71 (MQ=255) tctTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGt > 1:2732576/1‑71 (MQ=255) tctTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGt > 1:1357341/1‑71 (MQ=255) ctTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTg > 1:3868489/1‑71 (MQ=255) ttCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGa > 1:1266367/1‑63 (MQ=255) tCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGAcc > 1:983170/1‑47 (MQ=255) ccAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCa > 1:2178921/1‑53 (MQ=255) ccAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCa > 1:3862446/1‑53 (MQ=255) ccAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCg > 1:457572/1‑71 (MQ=255) cAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCg < 1:204308/70‑1 (MQ=255) gCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGc < 1:1124176/54‑1 (MQ=255) gCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTg < 1:3073086/65‑1 (MQ=255) cAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACcgc < 1:919426/44‑1 (MQ=255) cAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCg > 1:3319098/1‑71 (MQ=255) aaGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCgg > 1:2152902/1‑71 (MQ=255) aaGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCgg < 1:392242/71‑1 (MQ=255) aGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCggg < 1:912427/54‑1 (MQ=255) aGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCggg < 1:527136/54‑1 (MQ=255) aGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCggg < 1:3381616/54‑1 (MQ=255) aaCCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACtt > 1:2864650/1‑71 (MQ=255) aaCCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACtt < 1:3677117/71‑1 (MQ=255) aaCCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACt > 1:2323210/1‑70 (MQ=255) aCCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTg > 1:2667353/1‑71 (MQ=255) cGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGAGGGAACGGGTGGCGTTCGGCACTTGTa < 1:778230/71‑1 (MQ=255) cGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGAcc < 1:2020041/39‑1 (MQ=255) cGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTg > 1:2492891/1‑55 (MQ=255) ggATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGt < 1:310770/53‑1 (MQ=255) tGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCt < 1:1843732/70‑1 (MQ=255) gctcgctcGGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTc > 1:2059319/1‑71 (MQ=255) tcgctcgGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTc < 1:2216023/69‑1 (MQ=255) ctcgGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTaaa < 1:2010088/60‑1 (MQ=255) ctcgGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCAc > 1:3731307/1‑71 (MQ=255) ggCGTCAGGTACTGACCGCTACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACt < 1:643879/69‑1 (MQ=255) ggCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACt < 1:987381/69‑1 (MQ=255) gagTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACttt < 1:3267438/68‑1 (MQ=255) tCAGGTACTGATCGCGACCAATGCGGGAATGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGa > 1:669994/1‑71 (MQ=255) tCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGa < 1:687767/71‑1 (MQ=255) aGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATc < 1:3938695/71‑1 (MQ=255) aGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGAt < 1:2960908/70‑1 (MQ=255) ggTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATcc < 1:1787010/71‑1 (MQ=255) gTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATc > 1:2708969/1‑69 (MQ=255) aCTGATAGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACtt > 1:510199/1‑60 (MQ=255) aCTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATg > 1:1746318/1‑64 (MQ=255) cTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGc < 1:308402/70‑1 (MQ=255) tGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCt < 1:3893192/49‑1 (MQ=255) ccGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAAt > 1:3002189/1‑44 (MQ=255) | GGCAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGC > minE/219926‑220061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |