| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,727 | A→G | 11.7% | V114V (GTT→GTC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,727 | 0 | A | G | 11.7% | 157.7 / 11.1 | 60 | V114V (GTT→GTC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (26/27); new base G (4/3); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 | |||||||||||
GTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTT > minE/1015658‑1015792 | gtTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaa > 1:3869561/1‑71 (MQ=255) ttATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaa < 1:3554671/70‑1 (MQ=255) aGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat > 1:449900/1‑70 (MQ=255) acacCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTCTTACCAACaata < 1:612243/69‑1 (MQ=255) acCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACca < 1:670534/61‑1 (MQ=255) cgcgcgCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcgcg < 1:1651817/71‑1 (MQ=255) gcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaataat > 1:3655628/1‑62 (MQ=255) gcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaataat > 1:3707824/1‑62 (MQ=255) gcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGcc > 1:3082898/1‑71 (MQ=255) cgccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt > 1:3474463/1‑71 (MQ=255) cgccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt > 1:2402544/1‑71 (MQ=255) gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTa > 1:1417215/1‑69 (MQ=255) cgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat < 1:701938/51‑1 (MQ=255) cTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa > 1:2896941/1‑71 (MQ=255) tcatcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGcc < 1:1444709/59‑1 (MQ=255) catcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTgg < 1:2032231/68‑2 (MQ=255) tcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGt > 1:3633187/1‑71 (MQ=255) tcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGt > 1:847111/1‑71 (MQ=255) accaccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTg > 1:439802/1‑71 (MQ=255) accaccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTg < 1:2462578/71‑1 (MQ=255) ccaccaccGGTCCATACACGCTGCCCTTTTTTACcaa > 1:2020991/1‑37 (MQ=255) ccaccaccGGTCCATACACGCTGCCCATTATTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTg < 1:732587/70‑1 (MQ=255) caccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTgg > 1:224662/1‑70 (MQ=255) ccgccGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAg > 1:1350056/4‑71 (MQ=255) cGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACg > 1:290796/1‑71 (MQ=255) ggTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGc > 1:3045087/1‑71 (MQ=255) gTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGGGCCGGTATCCTGACAgg < 1:1781090/58‑1 (MQ=255) tCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCt > 1:1480523/1‑71 (MQ=255) tCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCt > 1:1415530/1‑71 (MQ=255) tCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCt < 1:33834/71‑1 (MQ=255) cATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGcc < 1:949855/68‑1 (MQ=255) aaTACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCa > 1:3489735/2‑61 (MQ=255) aTACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTc > 1:3650727/1‑71 (MQ=255) aTACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTc < 1:1151863/71‑1 (MQ=255) tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTa < 1:196216/43‑1 (MQ=255) tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATc < 1:2073736/45‑1 (MQ=255) tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCt < 1:3177596/47‑1 (MQ=255) tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa < 1:2229768/49‑1 (MQ=255) tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAg < 1:921888/60‑1 (MQ=255) tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCtt < 1:2996289/68‑1 (MQ=255) acgcGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTggc > 1:1523251/4‑71 (MQ=255) acacGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGtt < 1:2033882/54‑1 (MQ=255) acacGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgc > 1:599650/1‑71 (MQ=255) gcGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAg < 1:3920308/48‑1 (MQ=25) gCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACa < 1:568705/46‑1 (MQ=255) gCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACa < 1:3428337/46‑1 (MQ=255) cTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTa < 1:3925324/37‑1 (MQ=255) ggccTTTTTTACCGACGATAATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCAc > 1:2483132/3‑55 (MQ=255) gCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTCGCAGAACGcc > 1:2014258/1‑58 (MQ=255) cccTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATg < 1:2857399/70‑1 (MQ=255) cTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATg < 1:2847556/68‑1 (MQ=255) cTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCg > 1:1683019/1‑71 (MQ=255) ttttttACCAACAATAATCGCGGTGCCGGTATCCTGACAg < 1:3453674/40‑1 (MQ=255) ttttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcg > 1:415978/1‑59 (MQ=255) tttttACCGACGATGATCACGGTGCCGGTATCCTg > 1:1644390/1‑35 (MQ=19) tttttACCAACAATAATCGCGGTGCCGGTATCCTGACAg < 1:3451798/39‑1 (MQ=255) tttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCtt < 1:1053496/55‑1 (MQ=255) tttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgccgc > 1:3572142/1‑62 (MQ=255) ttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAg > 1:3476318/1‑71 (MQ=255) ttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAg > 1:1662617/1‑71 (MQ=255) tttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGt > 1:3416392/1‑71 (MQ=255) ttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGt < 1:1854993/70‑1 (MQ=255) tACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGtt < 1:2008371/70‑1 (MQ=255) tACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCgg < 1:1229421/66‑1 (MQ=255) | GTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTT > minE/1015658‑1015792 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |