Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,015,795 G→T 17.8% R92S (CGT→AGT) ‡ fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,015,7950GT17.8% 146.7 / 15.7 56R92S (CGT→AGT) ‡fumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base G (21/25);  new base T (7/3);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

TAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTG  >  minE/1015732‑1015864
                                                               |                                                                     
taatCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAAt                                                                >  1:3900731/1‑71 (MQ=255)
  atCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTg                                                              >  1:3847882/1‑71 (MQ=255)
     gcgGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTg                                                              >  1:79115/1‑68 (MQ=255)
     gcgGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCa                                                            <  1:361761/70‑1 (MQ=255)
       ggTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAgcg                                                         <  1:800964/71‑1 (MQ=255)
           ccGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCca                                                      >  1:2654439/1‑70 (MQ=255)
           ccGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCca                                                      >  1:3525072/1‑70 (MQ=255)
           ccGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCca                                                      >  1:294181/1‑70 (MQ=255)
               tATCCTTACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTg                                                              <  1:2764797/58‑1 (MQ=255)
                aTCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACatat                                                 >  1:1576625/1‑70 (MQ=255)
                      gcAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTcgt                                          <  1:1186741/70‑3 (MQ=255)
                        aGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGaa                                                                 >  1:923204/1‑46 (MQ=255)
                        aGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtt                                         >  1:1658305/1‑70 (MQ=255)
                           ttGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCg                                     <  1:3139360/71‑1 (MQ=255)
                           ttGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCg                                     <  1:2836493/71‑1 (MQ=255)
                           ttGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCg                                     <  1:2832034/71‑1 (MQ=255)
                           ttGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCg                                     <  1:476506/71‑1 (MQ=255)
                           ttGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCg                                     <  1:2719413/71‑1 (MQ=255)
                           ttGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCg                                     <  1:2065601/71‑1 (MQ=255)
                           tcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCg                                     >  1:1036365/3‑71 (MQ=255)
                           tcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCg                                     <  1:2486585/69‑1 (MQ=255)
                            tGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGaa                                                                 >  1:3794479/1‑42 (MQ=255)
                             ggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTcgt                                          >  1:2607250/1‑62 (MQ=255)
                             ggCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                    <  1:1344389/70‑1 (MQ=255)
                             ggCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCt                                   >  1:3631046/1‑71 (MQ=255)
                              gCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTg                                  >  1:529851/1‑71 (MQ=255)
                                 gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc                               >  1:100230/1‑71 (MQ=255)
                                  caCGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGct                               >  1:2195216/2‑69 (MQ=255)
                                  aaCGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAttt                                        <  1:1878438/61‑1 (MQ=255)
                                    cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAAt                                                                >  1:1677593/1‑35 (MQ=255)
                                    cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAAt                                                                >  1:1439749/1‑35 (MQ=255)
                                     gCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTctg                           >  1:3846492/1‑69 (MQ=255)
                                     gCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg                           <  1:1622153/71‑1 (MQ=255)
                                      ccTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCgg                          <  1:3641654/71‑1 (MQ=255)
                                      ccTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCgg                          <  1:2916760/71‑1 (MQ=255)
                                       cTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCg                                     <  1:2452771/59‑1 (MQ=255)
                                        tttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGt                        >  1:821981/1‑71 (MQ=255)
                                          tCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTg                                  >  1:586505/1‑59 (MQ=255)
                                           cgccgcGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                    >  1:945173/1‑56 (MQ=255)
                                              cgcgATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGAACTTGTCGTTTTCGCTGGCTTCTGGATcgtg                    >  1:1904568/1‑66 (MQ=255)
                                               gcgATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATcgtg                    >  1:50103/1‑65 (MQ=255)
                                               gcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAg                 <  1:284531/71‑1 (MQ=255)
                                               gcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAg                 <  1:1130330/71‑1 (MQ=255)
                                                cgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGa                <  1:1570174/71‑1 (MQ=255)
                                                cgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGa                <  1:2946017/71‑1 (MQ=255)
                                                  aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAAt              <  1:428186/71‑1 (MQ=255)
                                                   tGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCggg                         >  1:340474/1‑59 (MQ=255)
                                                    gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                    <  1:1947523/47‑1 (MQ=255)
                                                    gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTc                             <  1:2011663/54‑1 (MQ=255)
                                                        gAGTTACGCATGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGc        <  1:579382/71‑1 (MQ=255)
                                                        gAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCg          >  1:112155/1‑69 (MQ=255)
                                                        gAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGc        >  1:2103028/1‑71 (MQ=255)
                                                        gAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGc        <  1:2280586/71‑1 (MQ=255)
                                                          gTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCa      >  1:50623/1‑71 (MQ=255)
                                                           ttACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTACGGGTCACGCAGAATGTCGGCCAc     <  1:1390487/71‑1 (MQ=255)
                                                              cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtg  <  1:1286828/71‑1 (MQ=255)
                                                               |                                                                     
TAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTG  >  minE/1015732‑1015864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: