| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,835 | C→T | 14.3% | E78E (GAG→GAA) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,835 | 0 | C | T | 14.3% | 121.5 / 11.4 | 49 | E78E (GAG→GAA) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (14/28); new base T (4/3); total (18/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
GAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGA > minE/1015765‑1015904 | gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc > 1:100230/1‑71 (MQ=255) gCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTctg > 1:3846492/1‑69 (MQ=255) gCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg < 1:1622153/71‑1 (MQ=255) ccTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCgg < 1:2916760/71‑1 (MQ=255) ccTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCgg < 1:3641654/71‑1 (MQ=255) tttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGt > 1:821981/1‑71 (MQ=255) cgcgATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGAACTTGTCGTTTTCGCTGGCTTCTGGATcgtg > 1:1904568/1‑66 (MQ=255) gcgATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATcgtg > 1:50103/1‑65 (MQ=255) gcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAg < 1:1130330/71‑1 (MQ=255) gcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAg < 1:284531/71‑1 (MQ=255) cgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGa < 1:1570174/71‑1 (MQ=255) cgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGa < 1:2946017/71‑1 (MQ=255) aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAAt < 1:428186/71‑1 (MQ=255) tGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCggg > 1:340474/1‑59 (MQ=255) gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTc < 1:2011663/54‑1 (MQ=255) gAGTTACGCATGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGc < 1:579382/71‑1 (MQ=255) gAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCg > 1:112155/1‑69 (MQ=255) gAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGc > 1:2103028/1‑71 (MQ=255) gAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGc < 1:2280586/71‑1 (MQ=255) gTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCa > 1:50623/1‑71 (MQ=255) ttACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTACGGGTCACGCAGAATGTCGGCCAc < 1:1390487/71‑1 (MQ=255) cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtg < 1:1286828/71‑1 (MQ=255) cAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgtt < 1:2228343/71‑1 (MQ=255) cAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCAGGTCACGCAGAATGTCGGCCAcc < 1:2561581/67‑1 (MQ=255) ggAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGcc < 1:196257/62‑1 (MQ=255) ggAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt < 1:3369008/71‑1 (MQ=255) gAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCtgtttct < 1:1757065/71‑4 (MQ=255) aaTTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttg < 1:477566/68‑1 (MQ=255) aaTTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGttgttg < 1:277602/71‑1 (MQ=255) ttGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTgga < 1:2691527/40‑2 (MQ=12) ttGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGt > 1:578541/1‑71 (MQ=255) ttGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGt < 1:1844678/71‑1 (MQ=255) ttGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt > 1:1546073/1‑67 (MQ=255) gCAGCGCCACATATTTATCATTTTCGCTGGCCTCCgg > 1:1952346/1‑37 (MQ=255) cAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgcg < 1:3243852/71‑1 (MQ=255) cAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgcg < 1:246284/71‑1 (MQ=255) gcgcCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgcgcg > 1:3648842/1‑70 (MQ=255) cacaTATTTATCATTTTCGCTGGCCTCGGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGAc > 1:3078885/1‑71 (MQ=255) acaTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACg > 1:3829596/1‑71 (MQ=255) atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgca < 1:2138846/71‑1 (MQ=255) tttATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTc > 1:1970993/1‑39 (MQ=255) aTTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAac > 1:796910/1‑70 (MQ=255) aTTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAac < 1:80402/70‑1 (MQ=255) tttCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacga > 1:811236/1‑70 (MQ=255) gCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGca < 1:3807331/70‑1 (MQ=255) gCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGca < 1:158835/70‑1 (MQ=255) cTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGcatc < 1:2308537/71‑1 (MQ=255) cTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGcatc < 1:1524610/71‑1 (MQ=255) ggCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGcatcat > 1:1629260/1‑71 (MQ=255) gCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATg < 1:245173/71‑1 (MQ=255) cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacgacg < 1:638584/62‑1 (MQ=255) cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:1565932/70‑1 (MQ=255) | GAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGA > minE/1015765‑1015904 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |