| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,841 | G→A | 10.6% | D76D (GAC→GAT) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,841 | 0 | G | A | 10.6% | 130.1 / 5.7 | 47 | D76D (GAC→GAT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (15/27); new base A (3/2); total (18/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCC > minE/1015772‑1015910 | tttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGt > 1:821981/1‑71 (MQ=255) cgcgATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGAACTTGTCGTTTTCGCTGGCTTCTGGATcgtg > 1:1904568/1‑66 (MQ=255) gcgATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATcgtg > 1:50103/1‑65 (MQ=255) gcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAg < 1:1130330/71‑1 (MQ=255) gcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAg < 1:284531/71‑1 (MQ=255) cgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGa < 1:2946017/71‑1 (MQ=255) cgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGa < 1:1570174/71‑1 (MQ=255) aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAAt < 1:428186/71‑1 (MQ=255) tGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCggg > 1:340474/1‑59 (MQ=255) gAGTTACGCATGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGc < 1:579382/71‑1 (MQ=255) gAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCg > 1:112155/1‑69 (MQ=255) gAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGc < 1:2280586/71‑1 (MQ=255) gAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGc > 1:2103028/1‑71 (MQ=255) gTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCa > 1:50623/1‑71 (MQ=255) ttACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTACGGGTCACGCAGAATGTCGGCCAc < 1:1390487/71‑1 (MQ=255) cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtg < 1:1286828/71‑1 (MQ=255) cAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgtt < 1:2228343/71‑1 (MQ=255) cAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCAGGTCACGCAGAATGTCGGCCAcc < 1:2561581/67‑1 (MQ=255) ggAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGcc < 1:196257/62‑1 (MQ=255) ggAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt < 1:3369008/71‑1 (MQ=255) gAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCtgtttct < 1:1757065/71‑4 (MQ=255) aaTTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttg < 1:477566/68‑1 (MQ=255) aaTTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGttgttg < 1:277602/71‑1 (MQ=255) ttGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGt < 1:1844678/71‑1 (MQ=255) ttGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGt > 1:578541/1‑71 (MQ=255) ttGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt > 1:1546073/1‑67 (MQ=255) cAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgcg < 1:3243852/71‑1 (MQ=255) cAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgcg < 1:246284/71‑1 (MQ=255) gcgcCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgcgcg > 1:3648842/1‑70 (MQ=255) cacaTATTTATCATTTTCGCTGGCCTCGGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGAc > 1:3078885/1‑71 (MQ=255) acaTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACg > 1:3829596/1‑71 (MQ=255) atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgca < 1:2138846/71‑1 (MQ=255) tttATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTc > 1:1970993/1‑39 (MQ=255) aTTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAac < 1:80402/70‑1 (MQ=255) aTTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAac > 1:796910/1‑70 (MQ=255) tttCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacga > 1:811236/1‑70 (MQ=255) gCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGca < 1:158835/70‑1 (MQ=255) gCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGca < 1:3807331/70‑1 (MQ=255) cTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGcatc < 1:1524610/71‑1 (MQ=255) cTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGcatc < 1:2308537/71‑1 (MQ=255) ggCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGcatcat > 1:1629260/1‑71 (MQ=255) gCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATg < 1:245173/71‑1 (MQ=255) cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacgacg < 1:638584/62‑1 (MQ=255) cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:1565932/70‑1 (MQ=255) ccGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGcatcat > 1:1060536/1‑66 (MQ=255) cGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGc > 1:3310553/1‑60 (MQ=255) cGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGc > 1:2086710/1‑60 (MQ=255) cGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAAc < 1:108142/70‑1 (MQ=255) ggTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGcc > 1:2369084/1‑71 (MQ=255) | TTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCC > minE/1015772‑1015910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |