| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,017,194 | G→A | 12.0% | A310T (GCA→ACA) | manA → | mannose‑6‑phosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,017,194 | 0 | G | A | 12.0% | 155.1 / 10.6 | 58 | A310T (GCA→ACA) | manA | mannose‑6‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base G (29/22); new base A (4/3); total (33/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 | |||||||||||
CTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGAA > minE/1017125‑1017262 | cTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGc < 1:3434943/71‑1 (MQ=255) gTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAg < 1:2542374/70‑1 (MQ=255) cAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCGGCCGGTGAAACAAGGTGCAGAACTg > 1:2231681/1‑70 (MQ=255) cAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTg > 1:558381/1‑70 (MQ=255) cAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAAc > 1:3477218/1‑68 (MQ=255) aTGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGAc > 1:926206/1‑71 (MQ=255) aTGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGAc > 1:234715/1‑71 (MQ=255) tgtgAAATTCGAAGCCAAACAGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAACTGGACt > 1:2096693/1‑71 (MQ=255) tgAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTc < 1:1171663/71‑1 (MQ=255) aTTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCa > 1:1310915/1‑55 (MQ=255) aTTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGa > 1:3359387/1‑71 (MQ=255) ttCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGAt > 1:3351997/1‑71 (MQ=255) ttCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGAt > 1:1971291/1‑71 (MQ=255) tCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGAtt > 1:3361571/1‑71 (MQ=255) gAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGa > 1:888656/1‑67 (MQ=255) gAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTc > 1:3078940/1‑70 (MQ=255) ccAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTcc < 1:3731272/67‑1 (MQ=255) ccAAACCGGC‑AACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTg > 1:3864601/1‑70 (MQ=255) aaaCCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTg < 1:3882903/69‑1 (MQ=255) aaaCCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAACTGGACTTCCCGATTCCAGTGga < 1:2410041/71‑1 (MQ=255) aaCCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGgat > 1:2506117/1‑71 (MQ=255) aCCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGgat > 1:1830558/1‑70 (MQ=255) aCCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGgat > 1:1953684/1‑70 (MQ=255) ccGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTACAGAACTGGACTTCCCGAtt > 1:1370330/1‑60 (MQ=255) cTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAtttt < 1:1845297/71‑1 (MQ=255) cTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAtttt < 1:2958817/71‑1 (MQ=255) tAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAg > 1:3613155/1‑59 (MQ=255) aCCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGcc > 1:824929/1‑71 (MQ=255) aGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTtc < 1:1316856/71‑1 (MQ=255) gttgttGACCCAGCCGGTGAAACAAGGTACAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTtct > 1:2291322/1‑71 (MQ=255) ttgttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCt > 1:1934565/1‑67 (MQ=255) ttgttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCt > 1:3651466/1‑67 (MQ=255) ttgttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTtctc < 1:80998/71‑1 (MQ=255) tgttgACCCAGCCGGTGAAACAAGGTGCAGAACTg > 1:1310912/1‑35 (MQ=255) tgttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAtt > 1:1214638/1‑60 (MQ=255) gttgACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCg < 1:1807653/70‑1 (MQ=255) gACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCa < 1:1572234/46‑1 (MQ=255) gACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTg > 1:1027823/1‑70 (MQ=255) aCCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTtct < 1:2470160/64‑1 (MQ=255) aCCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGc < 1:2140267/70‑1 (MQ=255) aGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAttt > 1:674183/1‑52 (MQ=255) aGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAttt > 1:2938176/1‑52 (MQ=255) aGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGAc > 1:2253189/1‑71 (MQ=255) cGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCtt < 1:2768126/71‑1 (MQ=255) ggTGAAACAAGGTACAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTa < 1:3161604/71‑1 (MQ=255) gTGAAACAAGGTACAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGcc < 1:3872962/51‑1 (MQ=255) tGAAACAAGGTGCTGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCa < 1:3294512/61‑1 (MQ=255) tGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGc < 1:3063838/57‑1 (MQ=255) tGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGt < 1:1038486/71‑1 (MQ=255) aaaCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCtt < 1:3241889/50‑1 (MQ=255) aaaCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGACTTCTCGc > 1:196665/1‑55 (MQ=255) aaCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGa > 1:981975/1‑70 (MQ=255) aCAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAtt < 1:224164/41‑1 (MQ=255) cAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTtctc < 1:510817/50‑1 (MQ=255) aaGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTa > 1:50366/1‑63 (MQ=255) ggTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAg > 1:3728274/1‑70 (MQ=255) ggTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGa < 1:1976539/71‑1 (MQ=255) gTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGaa < 1:2170019/71‑1 (MQ=255) | CTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATTTTGCCTTCTCGCTGCATGACCTTAGTGATAAAGAA > minE/1017125‑1017262 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |