| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,019,201 | G→A | 42.8% | intergenic (+150/+76) | ydgA → / ← uidC | conserved hypothetical protein/predicted outer membrane porin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,019,201 | 0 | G | A | 42.8% | 20.4 / 53.2 | 49 | intergenic (+150/+76) | ydgA/uidC | conserved hypothetical protein/predicted outer membrane porin protein |
| Reads supporting (aligned to +/- strand): ref base G (12/16); new base A (6/15); total (18/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGT > minE/1019131‑1019270 | cTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTAcg < 1:1993423/71‑1 (MQ=255) ttATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTAcgc > 1:779747/1‑71 (MQ=255) ttATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTAcgc > 1:3282869/1‑71 (MQ=255) aGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCa < 1:2404620/70‑1 (MQ=255) ggCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATc > 1:2137630/1‑71 (MQ=255) ggCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATc > 1:26500/1‑71 (MQ=255) ggCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATc < 1:3546599/71‑1 (MQ=255) ggCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATc < 1:1245248/71‑1 (MQ=255) ctatgcctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATc < 1:2371030‑M2/14‑1 (MQ=255) ctatgcctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATc < 1:2375456‑M2/14‑1 (MQ=255) ctatgcctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATc < 1:3491278‑M2/14‑1 (MQ=255) tatgcctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATcc > 1:790998‑M2/57‑71 (MQ=255) atgcctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCg > 1:243104‑M2/56‑71 (MQ=255) tACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTAcgcc > 1:2541036/1‑62 (MQ=255) ctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCa > 1:1676041‑M2/52‑70 (MQ=255) ctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCAt < 1:3283024‑M2/20‑1 (MQ=255) ttttCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGc < 1:3815115/67‑1 (MQ=255) atgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaaa < 1:2519465‑M2/23‑1 (MQ=255) atgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaaa < 1:1079391‑M2/23‑1 (MQ=255) atgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaaa < 1:1089040‑M2/23‑1 (MQ=255) atgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaa < 1:2603307‑M2/22‑1 (MQ=255) ttCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTAcgcc > 1:564298/1‑56 (MQ=255) ttCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTAcgcc > 1:1302756/1‑56 (MQ=255) tCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATaaaa < 1:986075/70‑1 (MQ=255) gacgctgaaccgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCataaaaaca < 1:2384417‑M2/27‑3 (MQ=255) cTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑Ac < 1:3191438/71‑1 (MQ=255) cTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑Ac < 1:979563/71‑1 (MQ=255) acgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGcataaaaacac < 1:1965463‑M2/28‑4 (MQ=255) acgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATa < 1:297767‑M2/21‑1 (MQ=255) acgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCAGGCATa < 1:697423‑M2/21‑1 (MQ=255) cgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCg > 1:1409815‑M2/43‑58 (MQ=255) cAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑Acgcg > 1:2160539/1‑71 (MQ=255) tgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACAcgcg > 1:3902961‑M2/40‑70 (MQ=255) acTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATaa > 1:639830/1‑59 (MQ=255) aagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCa < 1:526000‑M2/33‑1 (MQ=255) aagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCAc > 1:1828998‑M2/38‑71 (MQ=255) cTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACtt < 1:988757/71‑1 (MQ=255) cTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACtt < 1:1905708/71‑1 (MQ=255) tttGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACttt > 1:414176/1‑71 (MQ=255) gtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCACTTTg < 1:2519438‑M2/38‑1 (MQ=255) gAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGt < 1:1289499/70‑1 (MQ=255) aaTTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTc < 1:3356061/70‑1 (MQ=255) gTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGa < 1:2413387/71‑1 (MQ=255) tttttGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTca < 1:1616320/61‑1 (MQ=255) ctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCACtt < 1:2384928‑M2/36‑1 (MQ=255) tttGTAGGCTGGATAAGGCGTTTATGCCGGATGCGGCGTaa > 1:1969139/1‑41 (MQ=14) tttGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTc > 1:1779547/1‑58 (MQ=255) tGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAAc < 1:2370010/69‑1 (MQ=255) tGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACg < 1:2009320/70‑1 (MQ=255) tGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACt > 1:604085/1‑44 (MQ=255) aaGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtc > 1:2418765/1‑71 (MQ=255) gTTTACGCCGCATCCGGCATAAACA‑ACGCGCACTTTGTCAGCAATCTGa > 1:3026504/1‑49 (MQ=11) ttACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTTAAACGCCGGAGATTTTTCTCTCCg > 1:3518056/1‑70 (MQ=255) tACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCgg < 1:3416868/70‑1 (MQ=255) tACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGc > 1:1989146/1‑71 (MQ=255) cgccgcATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATc > 1:1567664/1‑41 (MQ=255) gccgcATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGt > 1:2931215/1‑70 (MQ=255) | CTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAA‑ACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGT > minE/1019131‑1019270 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |