| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,026,302 | C→A | 10.8% | G190G (GGG→GGT) | malI ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,026,302 | 0 | C | A | 10.8% | 168.4 / 4.0 | 54 | G190G (GGG→GGT) | malI | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (30/18); new base A (4/2); total (34/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTG > minE/1026233‑1026370 | cAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGcc < 1:2229401/71‑1 (MQ=255)cAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGcc < 1:1309172/71‑1 (MQ=255) ggCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCt > 1:1663103/1‑71 (MQ=255) gCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTc > 1:1503653/1‑71 (MQ=255) aaTTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCccag > 1:789849/1‑71 (MQ=255) aaTTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCccag > 1:1789455/1‑71 (MQ=255) aTTTTAGTAGAGTTGCACAATAG‑CCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCccagc > 1:2010586/1‑70 (MQ=255) gtagAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGACCTCCCAGCCAgg < 1:2500748/69‑1 (MQ=255) gTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCg > 1:2083544/1‑71 (MQ=255) gTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGACCTCCCAGCCAGGCGATCCg > 1:1285583/1‑71 (MQ=255) gCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTc > 1:1283890/1‑52 (MQ=255) gCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt > 1:2054008/1‑70 (MQ=255) gCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:1412239/70‑1 (MQ=255) cacaATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGACCTCCCAGCCAGGCGATCCGCTg < 1:2742208/70‑1 (MQ=255) acaATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGAt < 1:1922430/71‑1 (MQ=255) aaTAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCccag < 1:1919419/52‑1 (MQ=255) aaTAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGACCTCCCAGCCAGGCGATCCGCTGATGc > 1:2603204/1‑71 (MQ=255) gCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGc > 1:3491560/1‑61 (MQ=255) ccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCg < 1:1238507/71‑1 (MQ=255) cAACCCGTTCTGCACGGGTTAATGAGGAACTTTGc > 1:1221726/1‑35 (MQ=255) cAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCg > 1:424506/1‑70 (MQ=255) cAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCg > 1:3583973/1‑70 (MQ=255) cAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCg > 1:3365254/1‑70 (MQ=255) cAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGa < 1:2172825/71‑1 (MQ=255) aaCCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGaa > 1:1184902/1‑71 (MQ=255) ccGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTg < 1:836067/54‑1 (MQ=255) cGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATgag < 1:1937077/71‑1 (MQ=255) cGTTCTGCACGGGTTAATGAGGAACTTTGACCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAAt > 1:2395582/1‑68 (MQ=255) gTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAAt > 1:3501025/1‑67 (MQ=255) ttCTGCACGGGTTAATGAGGAACTTTGCCCTCccagc < 1:238771/37‑1 (MQ=255) cTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGAGATCCGCTGATGCCCATTGCGAATGAGATGc > 1:3865367/1‑71 (MQ=255) tGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGa > 1:3628231/1‑49 (MQ=255) gCACGGGTTAATGAGGAACTTTGCCCTCccagccag > 1:3129795/1‑36 (MQ=255) cACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTcc > 1:3382371/1‑71 (MQ=255) cACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTcc > 1:3386974/1‑71 (MQ=255) gggTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAAt < 1:507955/58‑1 (MQ=255) tAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt > 1:381423/1‑38 (MQ=255) tAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTcc > 1:1305072/1‑64 (MQ=255) tAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac > 1:3522542/1‑70 (MQ=255) tAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac > 1:2251922/1‑70 (MQ=255) tGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:3455317/67‑1 (MQ=255) gAGGAACTTTGCCCTCGCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACt < 1:1583639/70‑1 (MQ=255) gAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaac < 1:3694202/66‑1 (MQ=255) aGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtg < 1:1305170/70‑1 (MQ=255) ggAACTTTGCCCTCCCATCCAGGCGATCCGCTGATGCCCAtt > 1:3575082/1‑42 (MQ=255) ggAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCAtt > 1:2021687/1‑42 (MQ=255) ggAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtgtg < 1:252787/71‑1 (MQ=255) ggAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtgtg < 1:1867891/71‑1 (MQ=255) taaCTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaacaa < 1:2130884/64‑1 (MQ=255) gAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTc < 1:3535180/60‑1 (MQ=255) aaCTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACtgtg > 1:1839693/1‑69 (MQ=255) aCTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGAt > 1:2788995/1‑50 (MQ=255) tttGACCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGcc > 1:3877911/1‑71 (MQ=255) ttGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaa > 1:1371500/1‑57 (MQ=255) ttGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTcaa > 1:3582647/1‑57 (MQ=255) tGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTg > 1:1416591/1‑71 (MQ=255) gCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTg < 1:1203645/70‑1 (MQ=255) | CAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGCTCCGTCAACAACTGTGCAGCCTG > minE/1026233‑1026370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |