| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,032,910 | T→C | 25.4% | intergenic (+55/‑31) | ydgT → / → ydgK | predicted regulator/conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,032,910 | 0 | T | C | 25.4% | 89.5 / 31.5 | 55 | intergenic (+55/‑31) | ydgT/ydgK | predicted regulator/conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (21/20); new base C (8/6); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTGGTTG > minE/1032840‑1032979 | gCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGAttt < 1:1550912/71‑1 (MQ=255) tGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGt > 1:110703/1‑71 (MQ=255) cAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGc > 1:2872880/1‑71 (MQ=255) cAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCACCTAAAATTAGATTCGCCTGTTTGc < 1:2364261/71‑1 (MQ=255) aTAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGt < 1:2150653/71‑1 (MQ=255) tAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTc > 1:2935347/1‑71 (MQ=255) tAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTCGCCTGTTTGCGTc > 1:3550564/1‑71 (MQ=255) gaaagTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTg > 1:1966710/1‑55 (MQ=255) gaaagTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTa > 1:824372/1‑71 (MQ=255) agTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTc > 1:790326/1‑71 (MQ=255) tAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTa > 1:103635/1‑66 (MQ=255) aTCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGc < 1:2143137/46‑1 (MQ=255) tCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAAc < 1:3833804/71‑1 (MQ=255) tCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAAc < 1:2341543/71‑1 (MQ=255) gTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTa < 1:3360359/71‑1 (MQ=255) gTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTa > 1:1606911/1‑71 (MQ=255) ttATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTg < 1:939692/44‑1 (MQ=255) ttATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCg < 1:3193577/50‑1 (MQ=255) ttATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTAt < 1:1783668/71‑1 (MQ=255) ttATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTAt < 1:3586392/71‑1 (MQ=255) tataAAAGCCTTATAATAACGCCCCTAAAATTAGATTCGCCTGTTTGCGTCGCTATTCt < 1:166642/59‑1 (MQ=255) tataAAAGCCTTATAATAACGCCCCTAAAATTAGATTCGCCTGTTTGCGTCGCTATTCTGGAGAACCTAt > 1:2152069/1‑70 (MQ=255) taAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGac < 1:3219258/71‑1 (MQ=255) taAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGac < 1:3214709/71‑1 (MQ=255) aaGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGacca > 1:3534440/1‑70 (MQ=255) cTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGt < 1:305158/41‑1 (MQ=255) cTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACcaccac > 1:608424/1‑70 (MQ=255) cTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCAccaca < 1:60493/71‑1 (MQ=255) cTTATAATAACGCCCCTAAAATTAGATTCGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCAccaca < 1:1485232/71‑1 (MQ=255) ataataACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTg > 1:2042706/1‑48 (MQ=255) ataataACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCAccacaacg > 1:2238319/1‑71 (MQ=255) taataaCGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCAccacaacg < 1:3837707/70‑1 (MQ=255) taataaCGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCAccacaacg < 1:130980/70‑1 (MQ=255) ataaCGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGCCCAccacaacgcca > 1:3596834/1‑71 (MQ=255) aCGCCCCTAAAATTAGATTCGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCAccacaacgccacaa > 1:896810/1‑71 (MQ=255) gCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCAccacaacgccacaacg < 1:2618901/71‑1 (MQ=255) ccccTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTg < 1:371076/39‑1 (MQ=255) cccTAAAATTAGATTCGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGAccacca > 1:3505795/1‑56 (MQ=255) ccTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTAt > 1:3892087/1‑71 (MQ=255) ccTAAAATTAGATTCGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACcaccac < 1:1446271/56‑1 (MQ=255) cTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGa < 1:1168315/48‑1 (MQ=255) cTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTAtt > 1:450640/1‑71 (MQ=255) tAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGAccacca > 1:2669825/1‑53 (MQ=255) aaaaTTAGATTCGCCTGTTTGCGTCGCTATTCTgg > 1:2160098/1‑35 (MQ=255) aaaTTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGaccac > 1:1347997/1‑49 (MQ=255) aaaTTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTg > 1:847463/1‑69 (MQ=255) aaaTTAGATTCGCCTGTTTGCGTCGCTATTCTGGAGAACCTa > 1:2822065/1‑42 (MQ=255) aaaTTAGATTCGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTa > 1:912888/1‑66 (MQ=255) aTTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGa < 1:1057829/43‑1 (MQ=255) aTTAGATTCGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCAccacaacgccaca < 1:964264/59‑1 (MQ=255) aTTAGATTCGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAg > 1:2102215/1‑70 (MQ=255) aGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGaccac > 1:2637612/1‑44 (MQ=255) aGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTg > 1:1023832/1‑64 (MQ=255) aGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTg > 1:276992/1‑64 (MQ=255) gATTCGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTgg < 1:947874/71‑1 (MQ=255) aTTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTGGt < 1:2937081/71‑1 (MQ=255) ttGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGa > 1:214139/1‑62 (MQ=255) ttGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGa > 1:209497/1‑62 (MQ=255) ttGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTGGt > 1:3026404/1‑69 (MQ=255) ttGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTGGt > 1:2998767/1‑69 (MQ=255) tGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTGGTTg < 1:110909/70‑1 (MQ=255) | GCACTATGTCCAATAAAGAAAGTAGCGATCGGTTATAAAAGCCTTATAATAACGCCCCTAAAATTAGATTTGCCTGTTTGCGTCGCTATTCTGGAGAACCTATGACCACCACAACGCCACAACGTATTGGAGGCTGGTTG > minE/1032840‑1032979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |