| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,050,725 | T→C | 25.4% | V305A (GTC→GCC) | ydhK → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,050,725 | 0 | T | C | 25.4% | 84.7 / 33.0 | 55 | V305A (GTC→GCC) | ydhK | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (23/18); new base C (9/5); total (32/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-01 | |||||||||||
GCGCTCGCCAGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAG > minE/1050655‑1050791 | gcgcTCGCCAGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGt < 1:1934015/71‑1 (MQ=255) gcTCGCCAGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCg < 1:3613487/71‑1 (MQ=255) cGCCAGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGcc > 1:669165/1‑70 (MQ=255) cGCCAGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGcc > 1:35200/1‑70 (MQ=255) gCCAGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGcc < 1:966899/69‑1 (MQ=255) gCCAGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGa > 1:3657242/1‑71 (MQ=255) cAGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACt < 1:2295473/71‑1 (MQ=255) tCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTc > 1:1301653/1‑60 (MQ=255) cGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCg < 1:1545687/70‑1 (MQ=255) gCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCg < 1:2543752/69‑1 (MQ=255) cAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGc < 1:314439/70‑1 (MQ=255) gATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGc > 1:3769046/1‑71 (MQ=255) aTGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGcc < 1:1590/71‑1 (MQ=255) tGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTAt < 1:3631222/58‑1 (MQ=255) tACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCCACGCCGCCGACTATCGGCACGTCGCCATCTg > 1:3153151/1‑71 (MQ=255) tACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCt < 1:2577585/70‑1 (MQ=255) cacCGTCGCACGTATTATCTCCCCGCTACGCCCGACCAACGTCGCCGa > 1:1098086/1‑48 (MQ=255) cacCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGa > 1:2373453/1‑48 (MQ=255) cacCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGa > 1:3901018/1‑48 (MQ=255) cacCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTgg > 1:1511297/1‑70 (MQ=255) cacCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGCCGCCGACTATCGGCACGTCGCCTTCTGGc < 1:3015222/71‑1 (MQ=255) acCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTgg > 1:3847414/1‑69 (MQ=255) acCGTCGCACGTATTATCGCACCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTgg > 1:1222515/1‑69 (MQ=255) cGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGc > 1:204310/1‑71 (MQ=255) cGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGc > 1:3927350/1‑71 (MQ=255) cGTATTATCGCCCCGCTACGCCCGACCAACGCCGCCGACTATCGGCa > 1:2327369/1‑47 (MQ=255) gTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGt > 1:546837/1‑71 (MQ=255) gTATTATCGCCCCGCTACGCCCGACCAACGCCGCCGACTATCGGCACg < 1:2339223/48‑1 (MQ=255) ttatCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAt > 1:2109643/1‑71 (MQ=255) tatCGCCCCGCTACGCCCGACCAACGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAtt > 1:3793627/1‑71 (MQ=255) atCGCCCCGCTACGCCCGACCAACGCCGCCGACTATCGGCa > 1:752516/1‑41 (MQ=255) atCGCCCCGCTACGCCCGACCAACGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAt > 1:667774/1‑69 (MQ=255) tCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGtt > 1:3422114/1‑66 (MQ=255) tCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAtttt < 1:1639329/71‑1 (MQ=255) tCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAttt < 1:218174/70‑1 (MQ=255) cGCCCCGCTACGCCCGACCAACGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGAc < 1:3675040/59‑1 (MQ=255) gCCCCGCTACGCCCGACCAACGCCGCCGACTATCg > 1:3905158/1‑35 (MQ=255) cccGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAttt < 1:115593/66‑1 (MQ=255) cccGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAttt < 1:2512878/66‑1 (MQ=255) gCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTgccgcc > 1:2960372/1‑71 (MQ=255) cTACGCCCGTCCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTgccgcc < 1:663104/70‑1 (MQ=255) cTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCt < 1:1578871/71‑1 (MQ=255) cGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTg < 1:594681/41‑1 (MQ=255) cGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTa < 1:693911/71‑1 (MQ=255) gCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTAt < 1:3862868/71‑1 (MQ=255) gCCCGACCAACGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTAt < 1:3837095/71‑1 (MQ=255) cccGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATc > 1:19045/1‑71 (MQ=255) ccGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTAttttt > 1:3045232/1‑58 (MQ=255) ccGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCt > 1:1230414/1‑71 (MQ=255) gACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATc > 1:80136/1‑68 (MQ=255) gACCAACGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGACTTTATCTg > 1:1199802/1‑70 (MQ=255) ccAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCa > 1:60516/1‑70 (MQ=255) cAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGc > 1:738973/1‑38 (MQ=255) cAACGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCa > 1:2202251/1‑69 (MQ=255) aaCGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAag > 1:703253/1‑71 (MQ=255) aCGCCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTgc < 1:3585058/53‑1 (MQ=255) | GCGCTCGCCAGTTCGCAAACAGATGTTTACACCGTCGCACGTATTATCGCCCCGCTACGCCCGACCAACGTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAG > minE/1050655‑1050791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |