| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,071,158 | A→G | 28.8% | Y4C (TAT→TGT) | mdtK → | multidrug efflux system transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,071,158 | 0 | A | G | 28.8% | 81.5 / 41.8 | 59 | Y4C (TAT→TGT) | mdtK | multidrug efflux system transporter |
| Reads supporting (aligned to +/- strand): ref base A (27/15); new base G (11/6); total (38/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01 | |||||||||||
GCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGC > minE/1071090‑1071227 | gctgcCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGtat < 1:1546739/70‑1 (MQ=255) cccATTCAGGAGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAg > 1:3292398/1‑71 (MQ=255) cATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGt > 1:777755/1‑70 (MQ=255) cAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAgc > 1:318381/1‑71 (MQ=255) gcTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGt < 1:3777969/69‑1 (MQ=255) gcTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGt < 1:3773546/69‑1 (MQ=255) cTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTg < 1:1044340/71‑1 (MQ=255) cTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTg < 1:1256265/71‑1 (MQ=255) tttttAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAgc < 1:1685053/63‑1 (MQ=255) tttttAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGcgcg < 1:1100386/66‑1 (MQ=255) tttttAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTc < 1:2740223/68‑1 (MQ=255) tttAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGtta > 1:1686073/1‑71 (MQ=255) tttAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGtta > 1:3907077/1‑71 (MQ=255) tttAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGtta > 1:1011853/1‑71 (MQ=255) tAGTCTCTCATGTAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGttat < 1:3927690/70‑1 (MQ=255) aGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGttat > 1:1018456/1‑69 (MQ=255) tctctcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGtata < 1:558754/46‑1 (MQ=255) tctctcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGtata < 1:804849/46‑1 (MQ=255) tctctcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTattagc < 1:121471/71‑1 (MQ=255) ctctcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTattagca > 1:1152004/1‑71 (MQ=255) tcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAg > 1:447629/1‑46 (MQ=255) tcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAg > 1:3611626/1‑46 (MQ=255) tcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAg > 1:2221259/1‑46 (MQ=255) tcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTattagcat < 1:729630/69‑1 (MQ=255) tcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATc < 1:2138184/44‑1 (MQ=255) tcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATc < 1:1625403/44‑1 (MQ=255) cATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTattagcattag > 1:748144/1‑71 (MQ=255) cATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTattagca > 1:1604871/1‑67 (MQ=255) atatAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTattagcatta < 1:2902967/69‑1 (MQ=255) tataACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTattagcattag > 1:2638146/1‑69 (MQ=255) taACTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCaa < 1:2953083/70‑1 (MQ=255) aCTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAAt > 1:3116788/1‑69 (MQ=255) aCTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAAt > 1:3321704/1‑69 (MQ=255) aCTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATcc > 1:2869005/1‑71 (MQ=255) aCTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATcc > 1:1572397/1‑71 (MQ=255) aCTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATcc > 1:748747/1‑71 (MQ=255) aCTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATc < 1:3142692/70‑1 (MQ=255) cTACAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATccc < 1:3756686/71‑1 (MQ=255) cTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGa < 1:3120169/41‑1 (MQ=255) cTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGa < 1:3743763/41‑1 (MQ=255) cTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGa < 1:3361380/41‑1 (MQ=255) aCAAATAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCg > 1:2469902/1‑70 (MQ=255) aaaTAAAAGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGt > 1:2024576/1‑70 (MQ=255) aaTAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGa > 1:2911130/1‑71 (MQ=255) aTAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTattagc < 1:958850/53‑1 (MQ=255) aTAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATccc > 1:124702/1‑65 (MQ=255) aTAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATcc > 1:16737/1‑64 (MQ=255) aaaaGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCt > 1:770056/1‑42 (MQ=255) aaaaGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCt > 1:3264177/1‑42 (MQ=255) aaaaGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCt > 1:316732/1‑42 (MQ=255) aaaGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATtct < 1:1133939/71‑1 (MQ=255) aGGTGTTCACGTGCAGAAGTGTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATtctc > 1:2553509/1‑70 (MQ=255) aGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATccc > 1:1149524/1‑60 (MQ=255) aGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATccc > 1:3846161/1‑60 (MQ=255) tgtTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTcgcg > 1:3492708/1‑70 (MQ=255) tCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGttat > 1:1442973/1‑38 (MQ=255) tCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCaa > 1:1624806/1‑70 (MQ=255) gTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGc > 1:884630/1‑71 (MQ=255) gCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGcc > 1:274410/1‑70 (MQ=255) cAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGccc > 1:3490051/1‑70 (MQ=255) aaGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGc > 1:270310/1‑65 (MQ=255) aaGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCaaa > 1:3139054/1‑70 (MQ=255) tataTCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGAtt < 1:2100105/48‑1 (MQ=255) atatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGc > 1:1561611/1‑70 (MQ=255) | GCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGC > minE/1071090‑1071227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |