| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,080,002 | G→A | 14.1% | P507L (CCA→CTA) | ydhV ← | predicted oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,080,002 | 0 | G | A | 14.1% | 179.2 / 18.2 | 71 | P507L (CCA→CTA) | ydhV | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (33/28); new base A (7/3); total (40/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
GTTTTTGGTTTCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACA > minE/1079932‑1080070 | gTTTTTGGTTTCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATg < 1:2834698/71‑1 (MQ=255)gTTTTTGGTTTCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATg > 1:2698887/1‑71 (MQ=255) tttGGTTTCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGc < 1:236892/70‑1 (MQ=255) ttGGTTTCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCa < 1:534044/70‑1 (MQ=255) tCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCgg > 1:291926/1‑70 (MQ=255) tCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCgg < 1:2506437/70‑1 (MQ=255) tCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCgg > 1:3844727/1‑70 (MQ=255) tCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGa < 1:3450454/68‑1 (MQ=255) tCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAAc < 1:1249713/70‑1 (MQ=255) cGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAAcc > 1:3604048/1‑70 (MQ=255) aaGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTT‑CAGTTTCAATGGCAAGCCGGAACCAATa > 1:2079257/1‑70 (MQ=255) aGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATaa < 1:1274652/71‑1 (MQ=255) gCATCTTCAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATa > 1:2832763/1‑70 (MQ=255) aTCTTCAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCa > 1:868232/1‑54 (MQ=38) aTCTTCAGAACCAAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAg > 1:3587773/1‑71 (MQ=255) aTCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGt < 1:3510901/71‑1 (MQ=255) aTCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGt > 1:163169/1‑71 (MQ=255) ttCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGa < 1:3523241/57‑1 (MQ=255) ttCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGa > 1:2049911/1‑71 (MQ=255) ttCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGa > 1:415837/1‑71 (MQ=255) cAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATg > 1:63639/1‑45 (MQ=255) aGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGatat > 1:2274679/1‑71 (MQ=255) aaCC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATgg > 1:1487156/1‑71 (MQ=255) aaCC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATgg > 1:3908691/1‑71 (MQ=255) cc‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGt < 1:1325850/71‑1 (MQ=255) c‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCAGGAACCAATAAAGTTGATATGGGTa < 1:3009436/71‑1 (MQ=255) aaaaaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTAt < 1:67687/71‑1 (MQ=255) aataGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCTAGGCGGAACCAATAAAGTTGat < 1:704822/59‑1 (MQ=255) aaaaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAg > 1:474967/1‑42 (MQ=255) aaaaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAg > 1:1802934/1‑42 (MQ=255) aaaaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGaa > 1:909804/1‑48 (MQ=255) aaaaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGaa > 1:3284312/1‑48 (MQ=255) aaaaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGat < 1:908320/62‑1 (MQ=255) aaaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGc > 1:458639/1‑71 (MQ=255) aaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAg > 1:2944297/1‑55 (MQ=255) aaGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAg > 1:768478/1‑55 (MQ=255) aGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGt > 1:2891842/1‑71 (MQ=255) aGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGt > 1:1196908/1‑71 (MQ=255) aGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGt > 1:2257651/1‑71 (MQ=255) aGTTCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAGTTGATATGGGt < 1:3657943/65‑1 (MQ=255) ttCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAg < 1:2577760/37‑1 (MQ=255) ttCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGatat < 1:2149765/59‑1 (MQ=255) tCTTTCGCCACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCAt > 1:2499112/1‑71 (MQ=255) cTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGtt > 1:2848415/1‑52 (MQ=255) tttCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCAt > 1:3771057/1‑69 (MQ=255) ttCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATg > 1:2823591/1‑69 (MQ=255) gCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGat < 1:3869691/50‑1 (MQ=255) gCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGat < 1:276392/50‑1 (MQ=255) ccACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAAt < 1:489815/40‑1 (MQ=255) cACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGatat > 1:318398/1‑50 (MQ=255) cACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCAc < 1:3427797/71‑1 (MQ=255) cACTTCACGTTGCAGTTTCAATAGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCa < 1:2254107/70‑1 (MQ=255) aCTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCGATAAAGTTGATATGGGTATGCGTCATGCAGTCAc < 1:418498/70‑1 (MQ=255) cTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGata > 1:3684652/1‑47 (MQ=255) cTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGata > 1:697270/1‑47 (MQ=255) cTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGata > 1:1009015/1‑47 (MQ=255) cTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATg < 1:1832776/62‑1 (MQ=255) cACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGtt < 1:393856/70‑1 (MQ=255) cACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGt < 1:937934/69‑1 (MQ=255) aCGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTg > 1:36015/1‑70 (MQ=255) tGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGt > 1:2524893/1‑50 (MQ=255) gTTTCAATAGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACa < 1:3517316/66‑1 (MQ=255) tttCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCg < 1:479349/44‑1 (MQ=255) tttCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGt > 1:48569/1‑71 (MQ=255) tttCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGt > 1:3579864/1‑71 (MQ=255) tCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTa > 1:3489822/1‑38 (MQ=255) tCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTa > 1:2470612/1‑38 (MQ=255) tCAATAGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCa > 1:1598487/1‑49 (MQ=255) tCAATAGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATAc > 1:3020371/1‑66 (MQ=255) tCAATAGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATAc > 1:1831863/1‑66 (MQ=255) cAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCa < 1:119304/44‑1 (MQ=255) cAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTa < 1:1938689/70‑1 (MQ=255) aaTGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAAc < 1:51123/71‑1 (MQ=255) aTGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACa < 1:905302/71‑1 (MQ=255) | GTTTTTGGTTTCATCGTAAGCATCTTCAGAACC‑AAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACA > minE/1079932‑1080070 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |