| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,094,503 | C→T | 11.1% | G625D (GGC→GAC) | ydiJ ← | predicted FAD‑linked oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,094,503 | 0 | C | T | 11.1% | 174.1 / 9.6 | 63 | G625D (GGC→GAC) | ydiJ | predicted FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (26/30); new base T (2/5); total (28/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGG > minE/1094435‑1094571 | gggCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAAcgtcg > 1:3080475/1‑71 (MQ=255) gCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAAcgccgc > 1:75052/1‑70 (MQ=255) aaTTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGAt > 1:3648652/1‑71 (MQ=255) gTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCcaa > 1:1392279/1‑71 (MQ=255) gTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCcaa > 1:2492405/1‑71 (MQ=255) cGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCcaaca > 1:2251576/1‑70 (MQ=255) aaaCTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGTCGCGATCCGCCAACAAACg < 1:3758430/71‑1 (MQ=255) aCTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCa < 1:3072291/71‑1 (MQ=255) aCGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCa < 1:2478778/71‑1 (MQ=255) gCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGa < 1:2675040/49‑1 (MQ=255) gCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCAtt < 1:1915391/71‑1 (MQ=255) ccGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTc < 1:3446182/71‑1 (MQ=255) cGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCa > 1:434979/1‑63 (MQ=255) gATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACaaa > 1:2763942/1‑57 (MQ=255) gATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACg > 1:2588924/1‑71 (MQ=255) aTTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACg > 1:3119406/1‑70 (MQ=255) aTTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACg > 1:2954642/1‑70 (MQ=255) aTTCAGGCAGTTCTTGTACCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCAc < 1:3829371/69‑1 (MQ=255) cAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCa > 1:2152655/1‑69 (MQ=255) cAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCAc < 1:2795586/70‑1 (MQ=255) cAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCAc > 1:2157181/1‑70 (MQ=255) ggCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAg < 1:3641019/71‑1 (MQ=255) gCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAg < 1:309167/70‑1 (MQ=255) gCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGc > 1:956588/1‑71 (MQ=255) cAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCg < 1:169689/71‑1 (MQ=255) gTTCTTGTTCCAGTTTGAGTGGATCACCGCCGCGATCCGCCAACACACGCAGCCATTCCCGCACCAGCGtt < 1:2361290/71‑1 (MQ=255) gTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCAt < 1:717951/56‑1 (MQ=255) gTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCAt < 1:2724263/56‑1 (MQ=255) gTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCAt < 1:1201735/56‑1 (MQ=255) gTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGtt < 1:1987988/71‑1 (MQ=255) gTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGtt < 1:2114990/71‑1 (MQ=255) ttCTTGTTCCAGTTTGAGTGGATCAACGTCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTg < 1:927814/71‑1 (MQ=255) ttCTTGTTCCAGTTTGAGTGGATCAACGTCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTg < 1:1925068/71‑1 (MQ=255) ttCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGtt < 1:1176657/70‑1 (MQ=255) ttCTTGTTCAAGTTTGAGTGGATCAACGCCGCGATCCGCcaac > 1:485402/1‑43 (MQ=255) tCTTGTTCCAGTTTGAGTGGATCAACGTCGCGATCCGCCAACAAACGCAGCCATTCACg < 1:3127855/59‑1 (MQ=255) cTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCg > 1:291135/1‑36 (MQ=255) cTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTgc < 1:2892144/70‑1 (MQ=255) ttGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCAc > 1:673348/1‑60 (MQ=255) ttGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTgcgc > 1:110517/1‑71 (MQ=255) gTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTgcgcgc > 1:3775494/1‑71 (MQ=255) ttCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCa < 1:2922459/59‑1 (MQ=255) ttCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCa < 1:118401/59‑1 (MQ=255) ttCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCa < 1:1941387/59‑1 (MQ=255) ttCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGAAGCCAtt < 1:2529205/50‑1 (MQ=255) ccAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCt > 1:2610892/1‑71 (MQ=255) ccAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCt > 1:2524577/1‑71 (MQ=255) ccAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCt > 1:3941024/1‑71 (MQ=255) tttGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCg < 1:2304008/71‑1 (MQ=255) ttGAGTGGATCAACGCCGCGATCCGCCAACAAACGCa > 1:2630733/1‑37 (MQ=255) ttGAGTGGATCAACGCCGCGATCCGCCAACAAACGCa > 1:274309/1‑37 (MQ=255) aGTGGATCAACGTCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGtg < 1:520769/70‑1 (MQ=255) aGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGtga < 1:2624009/71‑1 (MQ=255) aGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGtga < 1:2307524/71‑1 (MQ=255) aGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGtg < 1:2533764/70‑1 (MQ=255) tGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGtgaat < 1:1908795/71‑1 (MQ=255) ggATCAACGTCGCGATCCGCCAACAAACGCAGCCa > 1:515505/1‑35 (MQ=255) ggATCAACGTCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTc > 1:666689/1‑63 (MQ=255) gATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCAcc > 1:652845/1‑44 (MQ=255) aTCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGtgaatgaa < 1:7274/71‑1 (MQ=255) aTCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGtgaatgaa < 1:3563463/71‑1 (MQ=255) tCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTgaatgaat > 1:2223521/1‑71 (MQ=255) cAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCttt > 1:1608013/1‑58 (MQ=255) aaCGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATcc > 1:2820366/1‑71 (MQ=255) aaCGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATcc < 1:3428103/71‑1 (MQ=255) cgccgcGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCgg > 1:3014266/1‑71 (MQ=255) | GGGCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGG > minE/1094435‑1094571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |