| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,102,502 | T→C | 7.1% | F42F (TTT→TTC) | aroD → | 3‑dehydroquinate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,102,502 | 0 | T | C | 7.1% | 153.5 / 3.1 | 56 | F42F (TTT→TTC) | aroD | 3‑dehydroquinate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (17/35); new base C (2/2); total (19/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
GCTGATGGCGAAAGATATCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCATGGCGG > minE/1102439‑1102566 | gCTGATGGCGAAAGATATCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATAtt < 1:1724725/70‑1 (MQ=255)gCTGATGGCGAAAGATATCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATAtt < 1:1649164/70‑1 (MQ=255) aTGGCGAAAGATATCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGa < 1:1332209/71‑1 (MQ=255) tGGCGAAAGATATCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGaa < 1:2990502/71‑1 (MQ=255) ctaaaGATATCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATgg > 1:1688741/3‑71 (MQ=255) gAAAGATATCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTCGATATTCTGGAATGGc < 1:2873579/71‑1 (MQ=255) gAAAGATATCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTCGATATTCTGGAATGGc < 1:3183904/71‑1 (MQ=255) aaGATATCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATg > 1:1986589/1‑67 (MQ=255) aGATATCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTg > 1:116873/1‑61 (MQ=255) aGATATCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATg < 1:3248834/66‑1 (MQ=255) atatCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCtcg > 1:3949393/1‑58 (MQ=255) tatCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGTCTTTGATATTCTGGAATg > 1:541641/1‑63 (MQ=255) tatCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGtgtg < 1:3548342/70‑1 (MQ=255) gCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCg < 1:2322615/62‑1 (MQ=255) gCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCa < 1:2632548/71‑1 (MQ=255) gCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCa < 1:2580245/71‑1 (MQ=255) ccAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTg < 1:474588/53‑1 (MQ=255) cAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATAtt < 1:45094/49‑1 (MQ=255) cAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGtgtg < 1:316982/64‑1 (MQ=255) aGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATAtt < 1:2676444/48‑1 (MQ=255) aGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTa < 1:3230437/71‑1 (MQ=255) aGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTa < 1:494037/71‑1 (MQ=255) aGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTa < 1:1654465/71‑1 (MQ=255) gCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGat > 1:3947823/1‑44 (MQ=255) cGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATg > 1:2806927/1‑71 (MQ=255) cGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATg < 1:35060/71‑1 (MQ=255) gTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGtgtg < 1:3323431/60‑1 (MQ=255) tGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGcc > 1:3259637/1‑71 (MQ=255) tGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGcc < 1:994467/71‑1 (MQ=255) aaaTCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCg < 1:3167556/70‑1 (MQ=255) aaTCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGAc > 1:3673310/1‑71 (MQ=255) aTCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTgg < 1:1851695/44‑1 (MQ=255) ccGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGaa > 1:3814634/1‑44 (MQ=255) aaGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCtctc > 1:2843100/1‑70 (MQ=255) aaGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTcc < 1:1094597/71‑1 (MQ=255) aGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCa < 1:961549/71‑1 (MQ=255) ctctCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAAt < 1:2237551/71‑1 (MQ=255) tcGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGa > 1:1544073/1‑58 (MQ=255) tcGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAAtgtg > 1:426251/1‑71 (MQ=255) tcGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAAtg < 1:80234/69‑1 (MQ=255) gCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAAtgtg < 1:932337/69‑1 (MQ=255) gCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGa < 1:1251990/71‑1 (MQ=255) gCCTATCGTGAAGCGGACTTCGATATTCTGGAATGGCGTGTGGa > 1:2585012/1‑44 (MQ=255) gCCTATCGTGAAGCGGACTTCGATATTCTGGAATGGCGTGTGGAc > 1:1048390/1‑45 (MQ=255) cTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAAt > 1:3885462/1‑64 (MQ=255) tATCGTGAAGCGGACTTTGATATTCTGGAATGGCgtgt < 1:767894/38‑1 (MQ=255) gAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCAt > 1:2549757/1‑71 (MQ=255) aaGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCAt < 1:619583/70‑1 (MQ=255) aaGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCATg < 1:2470843/71‑1 (MQ=255) gCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAAt < 1:244540/54‑1 (MQ=255) cGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGAcc < 1:763874/45‑1 (MQ=255) ggACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGa < 1:1583703/57‑1 (MQ=255) ggACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGa < 1:2378391/57‑1 (MQ=255) ggACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCATggcgg < 1:1651369/71‑1 (MQ=255) aCTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCAt > 1:1354081/1‑64 (MQ=255) cTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCt > 1:1117190/1‑58 (MQ=255) tttGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGt < 1:1986390/59‑1 (MQ=255) tttGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCAt < 1:2274343/62‑1 (MQ=255) tttGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCAt < 1:1871847/62‑1 (MQ=255) tttGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCATggcgg < 1:524841/67‑1 (MQ=255) tttGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCATggcgg < 1:3575078/67‑1 (MQ=255) tGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCa > 1:1356865/1‑59 (MQ=255) tGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTATGTCa > 1:1490980/1‑59 (MQ=255) | GCTGATGGCGAAAGATATCGCCAGCGTGAAATCCGAAGCTCTCGCCTATCGTGAAGCGGACTTTGATATTCTGGAATGGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCATGGCGG > minE/1102439‑1102566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |