| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,179,532 | T→C | 43.5% | *328Q (TAA→CAA) | ansA → | cytoplasmic L‑asparaginase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,179,532 | 0 | T | C | 43.5% | 13.1 / 54.8 | 46 | *328Q (TAA→CAA) | ansA | cytoplasmic L‑asparaginase I |
| Reads supporting (aligned to +/- strand): ref base T (18/8); new base C (9/11); total (27/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTAATCGTGCTCACAT > minE/1179465‑1179589 | gCTAACCAAACTGCATTACCTGCTTAGCTAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCaa > 1:58204/1‑70 (MQ=255)gCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTaa > 1:1008528/1‑70 (MQ=255)gCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTaa > 1:3641307/1‑70 (MQ=255) aCCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAAcc < 1:605413/70‑1 (MQ=255) aCCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCaaa < 1:1412741/67‑1 (MQ=255) aCCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAAc < 1:3546076/69‑1 (MQ=255) cAAACTGCATTACCTGCTTAGACAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTgc > 1:193328/1‑71 (MQ=255) cAAACTGCATTACATGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCaaaa > 1:3277624/1‑66 (MQ=255) aaCTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTaaaa > 1:1786279/1‑64 (MQ=255) aaCTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTgcgc < 1:942166/71‑1 (MQ=255) aCTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGcgcg > 1:3244723/1‑71 (MQ=255) cATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCgcggcg < 1:368319/70‑1 (MQ=255) ttACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAAc < 1:389046/71‑1 (MQ=255) ttACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAAc > 1:3641139/1‑71 (MQ=255) aCCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAAcc > 1:2047378/1‑57 (MQ=255) ccTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCaaa < 1:3533053/53‑1 (MQ=255) ccTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCaaa < 1:1140260/53‑1 (MQ=255) ccTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCTGCGAACTg < 1:2181000/70‑1 (MQ=255) ccTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGa > 1:696434/1‑71 (MQ=255) ccTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGa > 1:3119199/1‑71 (MQ=255) cTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGAc < 1:1828396/71‑1 (MQ=255) cTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACg < 1:2990964/69‑1 (MQ=255) ttAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtt > 1:2269378/1‑71 (MQ=255) aGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTc > 1:3874412/1‑70 (MQ=255) ggAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCgcgg > 1:179321/1‑51 (MQ=255) ggATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAAc > 1:3489145/1‑50 (MQ=255) aTACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTgcg < 1:3080344/40‑1 (MQ=255) aTACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTgcg < 1:3546221/40‑1 (MQ=255) aTACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGAGAACTg < 1:4812/50‑1 (MQ=255) cTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACttt < 1:2603905/70‑1 (MQ=255) cTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACttt < 1:3140001/70‑1 (MQ=255) tGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTaa > 1:3764208/1‑71 (MQ=255) tGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTaa > 1:155447/1‑71 (MQ=255) gAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTg > 1:1826578/1‑53 (MQ=255) aatccATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTaa < 1:2001524/66‑1 (MQ=255) aaaCCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTAATc > 1:1262637/1‑71 (MQ=255) aaaCCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTAATc > 1:2406704/1‑71 (MQ=255) aaCCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTAATCg < 1:3302578/71‑1 (MQ=255) aaCCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTAATCg > 1:2613304/1‑71 (MQ=255) tCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTAATCGTGCTca > 1:684693/1‑71 (MQ=255) cGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTAATCGTGCTca > 1:153951/1‑70 (MQ=255) cGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTAATCGTGCTca > 1:1336465/1‑70 (MQ=255) cGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTAATCGTGCTca > 1:2291321/1‑70 (MQ=255) aaGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTAATCGTGCTCACAt > 1:671482/1‑70 (MQ=255) ggCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGc > 1:1991086/1‑40 (MQ=255) ccATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACttt < 1:336919/52‑1 (MQ=255) | GCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCTTTGCTCACATCTCACTTTAATCGTGCTCACAT > minE/1179465‑1179589 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |