| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,191,074 | A→C | 55.8% | intergenic (+415/+485) | yeaJ → / ← rnd | predicted diguanylate cyclase/ribonuclease D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,191,074 | 0 | A | C | 55.8% | 40.8 / 50.9 | 52 | intergenic (+415/+485) | yeaJ/rnd | predicted diguanylate cyclase/ribonuclease D |
| Reads supporting (aligned to +/- strand): ref base A (9/14); new base C (24/5); total (33/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.62e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
TGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCG > minE/1191010‑1191140 | tGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAgcgc > 1:1002260/1‑70 (MQ=255) gTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCCCAAgcg > 1:2719077/1‑68 (MQ=255) tCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTag < 1:2939628/71‑1 (MQ=255) ccATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTag > 1:2830873/1‑70 (MQ=255) aTCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTagcag > 1:2606585/1‑71 (MQ=255) aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGt < 1:229493/71‑1 (MQ=255) aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCg < 1:58912/70‑1 (MQ=255) gTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAgc > 1:2351800/1‑58 (MQ=255) gTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAgc < 1:1707042/58‑1 (MQ=255) gTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAgc < 1:1702516/58‑1 (MQ=255) gTTAAGCATCGGATGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGttt < 1:3093434/71‑1 (MQ=255) tAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTa > 1:335517/1‑71 (MQ=255) tAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTa > 1:3210262/1‑71 (MQ=255) tAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTa > 1:2894871/1‑71 (MQ=255) tAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTa > 1:76937/1‑71 (MQ=255) tAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTa > 1:2505196/1‑71 (MQ=255) aaGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTAc > 1:376754/1‑71 (MQ=255) aGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTAc < 1:136199/70‑1 (MQ=255) cATCGGCTGCGGTAAGCCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGt < 1:3406513/71‑1 (MQ=255) aTCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGt > 1:1568542/1‑70 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGa > 1:1608846/1‑69 (MQ=255) ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGaaa < 1:776269/71‑1 (MQ=255) cTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAg > 1:3541574/1‑70 (MQ=255) gTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGAtt < 1:1012947/71‑1 (MQ=255) aaGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc > 1:628412/1‑71 (MQ=255) aGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc > 1:1089398/1‑70 (MQ=255) cATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTa > 1:2649160/1‑71 (MQ=255) aTCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTAc > 1:395485/1‑71 (MQ=255) aTCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTAc > 1:1133451/1‑71 (MQ=255) cTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTAcc > 1:262735/1‑70 (MQ=255) ccGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCgg < 1:2454615/69‑1 (MQ=255) cAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAAc > 1:1203028/1‑71 (MQ=255) aaTGTCTGCGCTTTTTCCACCAGCGCTAGGAGCGTTTTACCGTGAAAGCGGATTTCGc > 1:2451093/1‑58 (MQ=255) aTGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAAcc > 1:1198065/1‑70 (MQ=255) aTGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAAcc > 1:1443649/1‑70 (MQ=255) aTGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGAt < 1:653230/52‑1 (MQ=255) gTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCg > 1:1042581/1‑47 (MQ=255) gTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACACAg > 1:10988/1‑71 (MQ=255) tCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTAc > 1:1857481/1‑57 (MQ=255) cgcTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGc < 1:3134546/68‑1 (MQ=255) cgcTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGc < 1:989355/68‑1 (MQ=255) cgcTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGc < 1:3279188/68‑1 (MQ=255) gcTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCAAGGCTGTc > 1:3083148/1‑71 (MQ=255) gcTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTc > 1:2622607/1‑71 (MQ=255) tttttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTAc < 1:793299/50‑1 (MQ=255) tttCCACCAGCGCTAGCAGCGTTTTACCGTGaaat > 1:3849953/1‑34 (MQ=37) tttCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTAc > 1:2770867/1‑48 (MQ=255) tttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAg > 1:81108/1‑70 (MQ=255) tttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGt > 1:118816/1‑71 (MQ=255) ttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCt < 1:93106/45‑1 (MQ=255) ttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGt < 1:1991591/70‑1 (MQ=255) ccACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTc < 1:2026585/70‑1 (MQ=255) cacaAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCg < 1:1524034/70‑1 (MQ=255) | TGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCG > minE/1191010‑1191140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |