Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,201,069	A→G	10.8%	E74E (GAA→GAG) ‡	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,201,069	0	A	G	10.8%	217.3 / 15.8	83	E74E (GAA→GAG) ‡	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base A (31/43);  new base G (5/4);  total (36/47)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

TTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTCTGCTGC  >  minE/1201000‑1201139                                                                      |                                                                       ttCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAAc                                                                       <  1:2552819/71‑1 (MQ=255) ttCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAAc                                                                       <  1:260318/71‑1 (MQ=255)   cACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCt                                                                     >  1:3847740/1‑71 (MQ=255)      tGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGc                                                                   >  1:3577907/1‑70 (MQ=255)        tCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAc                                                                >  1:1524997/1‑71 (MQ=255)        tCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAc                                                                >  1:3246124/1‑71 (MQ=255)          gCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAcc                                                               <  1:1479701/70‑1 (MQ=255)          gCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                                              <  1:2339332/71‑1 (MQ=255)          gCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                                              <  1:191605/71‑1 (MQ=255)               ccggTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGat                                                         <  1:2709983/69‑1 (MQ=255)               cGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGat                                                         <  1:2131114/71‑1 (MQ=255)                 ggTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGatat                                                       >  1:1541547/1‑71 (MQ=255)                  gTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGatat                                                       >  1:3463086/1‑70 (MQ=255)                  gTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATc                                                      >  1:3458611/1‑71 (MQ=255)                  gTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGatat                                                       >  1:3475882/1‑70 (MQ=255)                   tAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCg                                                     <  1:3424658/71‑1 (MQ=255)                   tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCt                                                                     <  1:1739371/55‑1 (MQ=255)                   tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATc                                                      >  1:2437087/1‑70 (MQ=255)                    aaaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCg                                                     >  1:3160451/1‑70 (MQ=255)                    aaaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                                    >  1:2807012/1‑71 (MQ=255)                    aaaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                                    >  1:1698745/1‑71 (MQ=255)                    aaaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                                    >  1:28292/1‑71 (MQ=255)                     aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                                              <  1:2795167/60‑1 (MQ=255)                     aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTg                                                            >  1:221149/1‑62 (MQ=255)                     aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                                    >  1:2524524/1‑70 (MQ=255)                     aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGAc                                                   <  1:1115253/71‑1 (MQ=255)                     aaGGCCACCACACCGATATCCCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                                              <  1:1499202/60‑1 (MQ=255)                      aGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGatt                                                  <  1:2122150/71‑3 (MQ=255)                      aGGCCACCACACCGATATCGCCA‑TATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                                 <  1:1121595/71‑1 (MQ=255)                       ggCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGc                                                                   >  1:2891181/1‑53 (MQ=255)                       ggCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCg                                                     <  1:693914/67‑1 (MQ=255)                       ggCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                                 <  1:242644/71‑1 (MQ=255)                        gCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGattc                                                 >  1:36065/1‑67 (MQ=255)                        gCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGattc                                                 >  1:2613467/1‑67 (MQ=255)                          caccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAcc                                                               <  1:1450100/54‑1 (MQ=255)                          caccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTAt                                              >  1:658597/1‑71 (MQ=255)                          caccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTAt                                              <  1:3356469/71‑1 (MQ=255)                          caccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTAt                                              <  1:1369986/71‑1 (MQ=255)                            ccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCg                                                     <  1:1035295/62‑1 (MQ=255)                              acacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAAc                                                                       >  1:850225/1‑42 (MQ=255)                               cacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTa                                               >  1:2320488/1‑65 (MQ=255)                               cacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTAtt                                             <  1:762724/67‑1 (MQ=255)                               cacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATAGACAGt                                                >  1:1564110/1‑64 (MQ=255)                                  cGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGtt                                      <  1:2998993/71‑1 (MQ=255)                                         gCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcg                               >  1:2261783/1‑71 (MQ=255)                                          ccATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTcag                              <  1:923037/71‑3 (MQ=255)                                             ttattaTGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGAc                           <  1:2480922/71‑1 (MQ=255)                                                 tatGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcgcg                             <  1:910709/65‑1 (MQ=255)                                                  atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                       <  1:1986121/70‑1 (MQ=255)                                                  atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                       <  1:3080664/70‑1 (MQ=255)                                                  atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                       <  1:2999549/70‑1 (MQ=255)                                                  atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                       >  1:2038524/1‑70 (MQ=255)                                                  atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                       <  1:765074/70‑1 (MQ=255)                                                  atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                       <  1:1646315/70‑1 (MQ=255)                                                   tGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTaga                      <  1:2338361/70‑1 (MQ=255)                                                   tGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTaga                      <  1:2166205/70‑1 (MQ=255)                                                   tGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTaga                      <  1:1195346/70‑1 (MQ=255)                                                      gTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAagag                  <  1:24227/71‑1 (MQ=255)                                                       tCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAagag                  <  1:2159079/70‑1 (MQ=255)                                                         ttGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgcgc               >  1:1185062/1‑71 (MQ=255)                                                          tGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgcgc               >  1:3573672/1‑70 (MQ=255)                                                          tGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgcgc               >  1:3569103/1‑70 (MQ=255)                                                           gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcgc                              >  1:3881240/1‑54 (MQ=255)                                                           gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgcgc               <  1:1174304/69‑1 (MQ=255)                                                           gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGcgcg              <  1:1485302/70‑1 (MQ=255)                                                           gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGcgcg              <  1:681754/70‑1 (MQ=255)                                                           gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATT‑‑CGACGTAGAAGAGCGCGAAc           >  1:2948877/1‑71 (MQ=255)                                                            cAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACg                          >  1:3610725/1‑57 (MQ=255)                                                            cAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGa             <  1:453335/70‑1 (MQ=255)                                                             aGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAAc           >  1:2086589/1‑71 (MQ=255)                                                              ggTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACg          <  1:2962713/71‑1 (MQ=255)                                                               gTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTagaa                     <  1:288050/59‑1 (MQ=255)                                                                tAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTagaa                     <  1:2480684/58‑1 (MQ=255)                                                                tAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTagaa                     <  1:66606/58‑1 (MQ=255)                                                                tAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgcgc               <  1:2145602/64‑1 (MQ=255)                                                                 aaCGAACCTGCCACCGTGGATATCGACAGTATTCCCGGtt                                      >  1:1307446/1‑40 (MQ=255)                                                                 aaCGAACCTGCCACCGTGGATATCGACAGTATTCCCGGtt                                      >  1:2614726/1‑40 (MQ=255)                                                                   cGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGt         >  1:3659357/1‑67 (MQ=255)                                                                   cGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgc     >  1:147160/1‑71 (MQ=255)                                                                   cGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgc     >  1:1764377/1‑71 (MQ=255)                                                                    gAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgct    <  1:697878/71‑1 (MQ=255)                                                                    gAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgct    <  1:1674090/71‑1 (MQ=255)                                                                    gAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgct    <  1:2278587/71‑1 (MQ=255)                                                                      aCCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAt                                  <  1:99089/39‑1 (MQ=255)                                                                      aCCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgctgc  >  1:50924/1‑71 (MQ=255)                                                                      aCCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgctgc  <  1:3620426/71‑1 (MQ=255)                                                                      |                                                                       TTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTCTGCTGC  >  minE/1201000‑1201139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑