Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,201,074	C→A	11.2%	A76D (GCC→GAC) ‡	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,201,074	0	C	A	11.2%	248.1 / 11.0	80	A76D (GCC→GAC) ‡	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base C (29/42);  new base A (5/4);  total (34/46)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

TGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTCTGCTGCT  >  minE/1201005‑1201140                                                                      |                                                                   tGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGc                                                                    >  1:3577907/1‑70 (MQ=255)   tCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAc                                                                 >  1:1524997/1‑71 (MQ=255)   tCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAc                                                                 >  1:3246124/1‑71 (MQ=255)     gCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAcc                                                                <  1:1479701/70‑1 (MQ=255)     gCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                                               <  1:2339332/71‑1 (MQ=255)     gCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                                               <  1:191605/71‑1 (MQ=255)          ccggTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGat                                                          <  1:2709983/69‑1 (MQ=255)          cGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGat                                                          <  1:2131114/71‑1 (MQ=255)            ggTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGatat                                                        >  1:1541547/1‑71 (MQ=255)             gTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGatat                                                        >  1:3463086/1‑70 (MQ=255)             gTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATc                                                       >  1:3458611/1‑71 (MQ=255)             gTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGatat                                                        >  1:3475882/1‑70 (MQ=255)              tAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCg                                                      <  1:3424658/71‑1 (MQ=255)              tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATc                                                       >  1:2437087/1‑70 (MQ=255)               aaaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCg                                                      >  1:3160451/1‑70 (MQ=255)               aaaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                                     >  1:28292/1‑71 (MQ=255)               aaaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                                     >  1:1698745/1‑71 (MQ=255)               aaaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                                     >  1:2807012/1‑71 (MQ=255)                aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                                               <  1:2795167/60‑1 (MQ=255)                aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTg                                                             >  1:221149/1‑62 (MQ=255)                aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                                     >  1:2524524/1‑70 (MQ=255)                aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGAc                                                    <  1:1115253/71‑1 (MQ=255)                aaGGCCACCACACCGATATCCCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                                               <  1:1499202/60‑1 (MQ=255)                 aGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGatt                                                   <  1:2122150/71‑3 (MQ=255)                 aGGCCACCACACCGATATCGCCA‑TATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                                  <  1:1121595/71‑1 (MQ=255)                  ggCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGc                                                                    >  1:2891181/1‑53 (MQ=255)                  ggCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCg                                                      <  1:693914/67‑1 (MQ=255)                  ggCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                                  <  1:242644/71‑1 (MQ=255)                   gCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGattc                                                  >  1:36065/1‑67 (MQ=255)                   gCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGattc                                                  >  1:2613467/1‑67 (MQ=255)                     caccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAcc                                                                <  1:1450100/54‑1 (MQ=255)                     caccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTAt                                               >  1:658597/1‑71 (MQ=255)                     caccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTAt                                               <  1:1369986/71‑1 (MQ=255)                     caccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTAt                                               <  1:3356469/71‑1 (MQ=255)                       ccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCg                                                      <  1:1035295/62‑1 (MQ=255)                          cacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTa                                                >  1:2320488/1‑65 (MQ=255)                          cacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTAtt                                              <  1:762724/67‑1 (MQ=255)                          cacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATAGACAGt                                                 >  1:1564110/1‑64 (MQ=255)                             cGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGtt                                       <  1:2998993/71‑1 (MQ=255)                                    gCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcg                                >  1:2261783/1‑71 (MQ=255)                                     ccATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTcag                               <  1:923037/71‑3 (MQ=255)                                        ttattaTGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGAc                            <  1:2480922/71‑1 (MQ=255)                                            tatGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcgcg                              <  1:910709/65‑1 (MQ=255)                                             atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                        <  1:765074/70‑1 (MQ=255)                                             atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                        <  1:2999549/70‑1 (MQ=255)                                             atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                        <  1:3080664/70‑1 (MQ=255)                                             atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                        <  1:1646315/70‑1 (MQ=255)                                             atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                        <  1:1986121/70‑1 (MQ=255)                                             atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                        >  1:2038524/1‑70 (MQ=255)                                              tGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTaga                       <  1:2338361/70‑1 (MQ=255)                                              tGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTaga                       <  1:2166205/70‑1 (MQ=255)                                              tGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTaga                       <  1:1195346/70‑1 (MQ=255)                                                 gTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAagag                   <  1:24227/71‑1 (MQ=255)                                                  tCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAagag                   <  1:2159079/70‑1 (MQ=255)                                                    ttGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgcgc                >  1:1185062/1‑71 (MQ=255)                                                     tGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgcgc                >  1:3573672/1‑70 (MQ=255)                                                     tGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgcgc                >  1:3569103/1‑70 (MQ=255)                                                      gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcgc                               >  1:3881240/1‑54 (MQ=255)                                                      gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgcgc                <  1:1174304/69‑1 (MQ=255)                                                      gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGcgcg               <  1:1485302/70‑1 (MQ=255)                                                      gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGcgcg               <  1:681754/70‑1 (MQ=255)                                                      gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATT‑‑CGACGTAGAAGAGCGCGAAc            >  1:2948877/1‑71 (MQ=255)                                                       cAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACg                           >  1:3610725/1‑57 (MQ=255)                                                       cAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGa              <  1:453335/70‑1 (MQ=255)                                                        aGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAAc            >  1:2086589/1‑71 (MQ=255)                                                         ggTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACg           <  1:2962713/71‑1 (MQ=255)                                                          gTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTagaa                      <  1:288050/59‑1 (MQ=255)                                                           tAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTagaa                      <  1:2480684/58‑1 (MQ=255)                                                           tAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTagaa                      <  1:66606/58‑1 (MQ=255)                                                           tAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgcgc                <  1:2145602/64‑1 (MQ=255)                                                            aaCGAACCTGCCACCGTGGATATCGACAGTATTCCCGGtt                                       >  1:1307446/1‑40 (MQ=255)                                                            aaCGAACCTGCCACCGTGGATATCGACAGTATTCCCGGtt                                       >  1:2614726/1‑40 (MQ=255)                                                              cGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGt          >  1:3659357/1‑67 (MQ=255)                                                              cGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgc      >  1:1764377/1‑71 (MQ=255)                                                              cGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgc      >  1:147160/1‑71 (MQ=255)                                                               gAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgct     <  1:2278587/71‑1 (MQ=255)                                                               gAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgct     <  1:697878/71‑1 (MQ=255)                                                               gAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgct     <  1:1674090/71‑1 (MQ=255)                                                                 aCCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAt                                   <  1:99089/39‑1 (MQ=255)                                                                 aCCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgctgc   >  1:50924/1‑71 (MQ=255)                                                                 aCCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgctgc   <  1:3620426/71‑1 (MQ=255)                                                                  ccTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgctgct  >  1:622907/1‑71 (MQ=255)                                                                      |                                                                   TGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTCTGCTGCT  >  minE/1201005‑1201140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑