| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,211,202 | A→T | 8.7% | V50V (GTT→GTA) | cspC ← | stress protein, member of the CspA‑family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,211,202 | 0 | A | T | 8.7% | 192.2 / 9.1 | 69 | V50V (GTT→GTA) | cspC | stress protein, member of the CspA‑family |
| Reads supporting (aligned to +/- strand): ref base A (29/34); new base T (3/3); total (32/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTT > minE/1211135‑1211272 | gATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACg > 1:3090982/1‑71 (MQ=255)gATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACg < 1:431366/71‑1 (MQ=255) aTTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGt < 1:3835973/71‑1 (MQ=255) gATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCt < 1:1105075/70‑1 (MQ=255) gATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCt < 1:748010/70‑1 (MQ=255) gATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCt < 1:879768/70‑1 (MQ=255) aGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCt < 1:3007819/71‑1 (MQ=255) gATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCtt < 1:935294/71‑1 (MQ=255) tAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCtt > 1:2449383/1‑69 (MQ=255) aGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGa > 1:3587162/1‑64 (MQ=255) cTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGa < 1:853926/62‑1 (MQ=255) aCGTTAACAGCTGCCGGACCTTTGTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGt > 1:3797890/1‑71 (MQ=255) aCGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGt > 1:2123489/1‑71 (MQ=255) tAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCa < 1:1509052/59‑1 (MQ=255) aaCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg < 1:873703/70‑1 (MQ=255) aaCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg < 1:1399248/70‑1 (MQ=255) aCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg > 1:1160875/1‑69 (MQ=255) aCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa < 1:359650/71‑1 (MQ=255) cAGCTGCCGGACCTTTCTGGCCTTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGtt < 1:3585270/65‑1 (MQ=255) cAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGa > 1:1860093/1‑69 (MQ=255) cAGCTGCCGGACCTTTCTGGCCGTCATGAAATTAGAACTCAACGt > 1:2425342/1‑45 (MQ=255) cTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCaa > 1:2057414/1‑39 (MQ=255) cTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCa > 1:1055219/1‑71 (MQ=255) tGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt > 1:886128/1‑71 (MQ=255) tGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt > 1:2308363/1‑71 (MQ=255) ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt < 1:3759316/69‑1 (MQ=255) ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa > 1:2699652/1‑71 (MQ=255) ccGGACCTTTCTGGCCGTCCTGAATCTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAtt < 1:531293/70‑1 (MQ=255) ccGGACCTTTCTGGACGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa < 1:2236277/71‑1 (MQ=255) ggACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCa > 1:2800256/1‑66 (MQ=255) gACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCaga < 1:1283729/53‑1 (MQ=255) ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa > 1:2902704/1‑59 (MQ=255) ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg > 1:899542/1‑71 (MQ=255) ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg > 1:778458/1‑71 (MQ=255) tttCTGGCCGTCCTGAATTTCGAACTCAACGTTCt < 1:1051872/35‑1 (MQ=255) ttCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCa > 1:1354117/1‑60 (MQ=255) tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTc > 1:3051221/1‑40 (MQ=255) tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa < 1:690552/69‑1 (MQ=255) cTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATa < 1:3792426/70‑1 (MQ=255) cTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATa < 1:725742/70‑1 (MQ=255) gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGc < 1:3019907/69‑1 (MQ=255) gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:2478583/70‑1 (MQ=255) gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag > 1:1478149/1‑70 (MQ=255) cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGcc < 1:3159318/48‑1 (MQ=255) tGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGttt > 1:2751592/1‑40 (MQ=255) tGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGttt > 1:186154/1‑40 (MQ=255) gtgaTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAgtg < 1:477945/68‑1 (MQ=255) aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTAc > 1:1059989/1‑71 (MQ=255) tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg > 1:217490/1‑52 (MQ=255) ttCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa < 1:706655/68‑1 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg < 1:1915725/36‑1 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGaa > 1:156016/1‑62 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGaa > 1:2571572/1‑62 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGa < 1:176980/70‑1 (MQ=255) cGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGaa > 1:1637821/1‑70 (MQ=255) gAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAac < 1:1652031/70‑1 (MQ=255) gAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc < 1:1687002/38‑1 (MQ=255) gAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc < 1:1179802/38‑1 (MQ=255) gAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc < 1:1420971/38‑1 (MQ=255) aaCTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAacac > 1:372179/1‑71 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg > 1:1054772/1‑47 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAacac < 1:24290/71‑1 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca > 1:2495753/1‑70 (MQ=255) aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAacac < 1:3755859/70‑1 (MQ=255) aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAAcaca > 1:3192446/1‑71 (MQ=255) aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAAcaca > 1:2657771/1‑71 (MQ=255) cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc < 1:2087680/42‑1 (MQ=255) cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:933161/52‑1 (MQ=255) cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAAcaca > 1:3527977/1‑70 (MQ=255) tCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGaa < 1:605378/56‑1 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:1478301/1‑39 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGTAGAAGTGTACGAACACATCt < 1:2715024/70‑1 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt > 1:3407707/1‑71 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGAGTACGAACACATCt > 1:103504/1‑70 (MQ=255) | GATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTT > minE/1211135‑1211272 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |