| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,211,229 | G→A | 12.9% | F41F (TTC→TTT) | cspC ← | stress protein, member of the CspA‑family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,211,229 | 0 | G | A | 12.9% | 225.7 / 20.4 | 85 | F41F (TTC→TTT) | cspC | stress protein, member of the CspA‑family |
| Reads supporting (aligned to +/- strand): ref base G (35/39); new base A (7/4); total (42/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
AACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAG > minE/1211160‑1211298 | aaCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg < 1:873703/70‑1 (MQ=255)aaCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg < 1:1399248/70‑1 (MQ=255) aCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg > 1:1160875/1‑69 (MQ=255) aCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa < 1:359650/71‑1 (MQ=255) cAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGa > 1:1860093/1‑69 (MQ=255) cTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCa > 1:1055219/1‑71 (MQ=255) tGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt > 1:2308363/1‑71 (MQ=255) tGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt > 1:886128/1‑71 (MQ=255) ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt < 1:3759316/69‑1 (MQ=255) ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa > 1:2699652/1‑71 (MQ=255) ccGGACCTTTCTGGCCGTCCTGAATCTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAtt < 1:531293/70‑1 (MQ=255) ccGGACCTTTCTGGACGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa < 1:2236277/71‑1 (MQ=255) ggACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCa > 1:2800256/1‑66 (MQ=255) ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa > 1:2902704/1‑59 (MQ=255) ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg > 1:778458/1‑71 (MQ=255) ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg > 1:899542/1‑71 (MQ=255) ttCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCa > 1:1354117/1‑60 (MQ=255) tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa < 1:690552/69‑1 (MQ=255) cTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATa < 1:725742/70‑1 (MQ=255) cTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATa < 1:3792426/70‑1 (MQ=255) gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGc < 1:3019907/69‑1 (MQ=255) gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:2478583/70‑1 (MQ=255) gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag > 1:1478149/1‑70 (MQ=255) cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGcc < 1:3159318/48‑1 (MQ=255) gtgaTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAgtg < 1:477945/68‑1 (MQ=255) aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTAc > 1:1059989/1‑71 (MQ=255) tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg > 1:217490/1‑52 (MQ=255) ttCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa < 1:706655/68‑1 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg < 1:1915725/36‑1 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGaa > 1:2571572/1‑62 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGaa > 1:156016/1‑62 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGa < 1:176980/70‑1 (MQ=255) cGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGaa > 1:1637821/1‑70 (MQ=255) gAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAac < 1:1652031/70‑1 (MQ=255) gAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc < 1:1420971/38‑1 (MQ=255) gAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc < 1:1179802/38‑1 (MQ=255) gAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc < 1:1687002/38‑1 (MQ=255) aaCTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAacac > 1:372179/1‑71 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg > 1:1054772/1‑47 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAacac < 1:24290/71‑1 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca > 1:2495753/1‑70 (MQ=255) aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAacac < 1:3755859/70‑1 (MQ=255) aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAAcaca > 1:2657771/1‑71 (MQ=255) aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAAcaca > 1:3192446/1‑71 (MQ=255) cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc < 1:2087680/42‑1 (MQ=255) cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:933161/52‑1 (MQ=255) cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAAcaca > 1:3527977/1‑70 (MQ=255) tCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGaa < 1:605378/56‑1 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:1478301/1‑39 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGTAGAAGTGTACGAACACATCt < 1:2715024/70‑1 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt > 1:3407707/1‑71 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGAGTACGAACACATCt > 1:103504/1‑70 (MQ=255) aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAtt < 1:269154/35‑1 (MQ=255) aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAGGTGTACGAACACATCttt < 1:947240/71‑1 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACTCATCTTtg > 1:1761059/1‑71 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtg > 1:3923645/1‑71 (MQ=255) tttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAGTGTa < 1:2721220/55‑1 (MQ=255) gTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgc > 1:637407/1‑71 (MQ=255) ttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAGTGTACGAacactga < 1:621917/65‑4 (MQ=255) ttCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:2999891/45‑1 (MQ=255) ttCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:1544182/45‑1 (MQ=255) ttCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:1548692/45‑1 (MQ=255) cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg < 1:1901325/70‑1 (MQ=255) tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACAt > 1:2409280/1‑61 (MQ=255) gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa > 1:582459/1‑71 (MQ=255) aCCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa < 1:543206/70‑1 (MQ=255) aCCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGcc < 1:1559666/69‑1 (MQ=25) ccTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCAt > 1:3633317/1‑70 (MQ=255) cTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGaa < 1:3806519/52‑1 (MQ=255) cTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCAt > 1:1249309/1‑69 (MQ=255) tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca > 1:223531/1‑52 (MQ=255) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAac > 1:111609/1‑51 (MQ=255) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGcc > 1:440163/1‑65 (MQ=19) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTcttc > 1:3353712/1‑68 (MQ=16) aGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCg < 1:1982487/71‑1 (MQ=255) aGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCg < 1:77269/71‑1 (MQ=255) aGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCg > 1:2785260/1‑71 (MQ=255) gCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCgg < 1:1518876/71‑1 (MQ=255) ccAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGa < 1:559992/71‑1 (MQ=255) ccAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAGTGTACGAacact < 1:3563071/50‑2 (MQ=255) ccAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAGTGTACGAacact < 1:2276615/50‑2 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg > 1:1540361/1‑70 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt < 1:2122453/71‑1 (MQ=255) agTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTaa > 1:629527/1‑71 (MQ=255) agTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTaa > 1:2564493/1‑71 (MQ=255) ttttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg < 1:1300803/71‑1 (MQ=255) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg > 1:689997/1‑69 (MQ=255) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg > 1:3587239/1‑69 (MQ=255) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa < 1:3211159/70‑1 (MQ=255) gAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAg < 1:2114138/70‑1 (MQ=255) | AACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAG > minE/1211160‑1211298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |