| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 223,030 | T→G | 7.4% | intergenic (+85/‑95) | lpcA → / → prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 223,030 | 0 | T | G | 7.4% | 242.2 / 13.2 | 94 | intergenic (+85/‑95) | lpcA/prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Reads supporting (aligned to +/- strand): ref base T (40/47); new base G (3/4); total (43/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
GGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGAAAATTCTT > minE/222960‑223099 | ggACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGc < 1:2257632/71‑1 (MQ=2) aTAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGc > 1:368178/1‑71 (MQ=255) aggcGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTa > 1:3539000/4‑71 (MQ=11) aggcGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCt < 1:330049/67‑1 (MQ=11) aggcGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCt < 1:1592322/67‑1 (MQ=11) aggcGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCt < 1:1566096/67‑1 (MQ=11) aggcGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTa > 1:2336579/4‑71 (MQ=11) gACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTa > 1:1374841/1‑70 (MQ=255) aCGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCTTGCCTACa < 1:1527663/71‑1 (MQ=255) cGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACaa < 1:2412485/71‑1 (MQ=14) cGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACa > 1:1412261/1‑70 (MQ=255) gTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACaaa > 1:770164/1‑71 (MQ=255) tttACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAAt < 1:2860960/71‑1 (MQ=255) ttACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc > 1:2275068/1‑71 (MQ=255) aCGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAttcc > 1:2556266/1‑68 (MQ=11) aCGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGACCTACAAAttct < 1:3662595/71‑4 (MQ=11) gccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTa > 1:323003/1‑71 (MQ=255) ccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAc < 1:3931188/71‑1 (MQ=255) tgcATCCGGCA‑CTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa < 1:442082/57‑1 (MQ=14) gcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgc > 1:1371132/1‑71 (MQ=255) gcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgc > 1:2146681/1‑71 (MQ=255) aTCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGa < 1:483843/71‑1 (MQ=255) tCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAt > 1:747356/1‑60 (MQ=11) ccGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAAt > 1:457429/1‑59 (MQ=255) gCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCAtcc > 1:2705439/1‑46 (MQ=255) cATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg > 1:1868962/1‑69 (MQ=255) cATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg > 1:2067074/1‑69 (MQ=255) cATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTa < 1:2612615/71‑1 (MQ=255) aTTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGt < 1:381638/69‑1 (MQ=255) aTTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAg > 1:1276661/1‑71 (MQ=255) actgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCt < 1:3002515/45‑1 (MQ=14) actgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCt < 1:225542/45‑1 (MQ=14) ttGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGc > 1:768215/1‑71 (MQ=255) tcgtgCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACa < 1:905556/47‑1 (MQ=11) cgtgCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACa < 1:430042/47‑1 (MQ=11) tgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCg < 1:2390605/70‑1 (MQ=255) gcgcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaa > 1:2012727/1‑71 (MQ=255) cgcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaat < 1:3570070/71‑1 (MQ=255) gcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaata < 1:2282270/71‑1 (MQ=255) ctgatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaataa > 1:3428121/1‑71 (MQ=255) ctatgcctgatgcgaCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatc < 1:2371030‑M1/69‑31 (MQ=255) ctatgcctgatgcgaCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatc < 1:2375456‑M1/69‑31 (MQ=255) ctatgcctgatgcgaCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatc < 1:3491278‑M1/69‑31 (MQ=255) tatgcctgatgcgACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatcc > 1:790998‑M1/2‑40 (MQ=255) gatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATg > 1:1990384/1‑71 (MQ=255) atgcctgatgcGACGCTGACGCGTTTTATCATGCCTAc < 1:2269318/38‑1 (MQ=255) atgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaa < 1:2156567/65‑1 (MQ=255) atgcctgatgcGACGCTGAAGCGTCTTATCATGCCTAc > 1:74542/1‑38 (MQ=255) atgcctgatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccg > 1:243104‑M1/1‑39 (MQ=255) tgcctgatgcGACGCTGACGCGTTTTATCATGCCt < 1:973100/35‑1 (MQ=255) ctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgcg < 1:1743280/47‑1 (MQ=255) ctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgcg < 1:3070658/47‑1 (MQ=255) ctgatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcat < 1:3283024‑M1/71‑37 (MQ=255) ctgatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggca > 1:1676041‑M1/1‑35 (MQ=255) tgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaa > 1:2771634/1‑60 (MQ=255) tgatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtt < 1:1167118‑M1/53‑20 (MQ=255) gatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgt > 1:731175‑M1/1‑33 (MQ=255) atgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAcg < 1:618101/71‑1 (MQ=255) atgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaa < 1:2519465‑M1/71‑40 (MQ=255) atgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaa < 1:1089040‑M1/71‑40 (MQ=255) atgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaa < 1:1079391‑M1/71‑40 (MQ=255) atgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataa < 1:2603307‑M1/70‑39 (MQ=255) tgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAcgc > 1:1892864/1‑71 (MQ=255) tgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAcg > 1:1441439/1‑70 (MQ=255) gcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAtgcc > 1:3115940/1‑71 (MQ=255) gACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAcgccg > 1:596923/1‑70 (MQ=255) gACGCTGAACCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaaca < 1:2384417‑M1/71‑44 (MQ=255) aCGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCa < 1:72554/71‑1 (MQ=255) aCGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCa > 1:3291355/1‑71 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacac < 1:1965463‑M1/71‑45 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcata < 1:297767‑M1/64‑38 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatcaggcata < 1:697423‑M1/64‑38 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccac < 1:1691388‑M1/39‑13 (MQ=255) cGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccg > 1:1409815‑M1/1‑26 (MQ=255) cGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtat > 1:360096‑M1/1‑26 (MQ=255) cTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATcc < 1:2250511/71‑1 (MQ=255) tGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATcc > 1:2119144/1‑70 (MQ=255) tGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcg > 1:3902961‑M1/1‑23 (MQ=255) aagcgtcTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcgcac > 1:1828998‑M1/3‑21 (MQ=255) aagcgtcTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcgca < 1:526000‑M1/68‑50 (MQ=255) cgcgTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAcgc > 1:2417102/1‑60 (MQ=255) cgTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTg < 1:2875335/71‑1 (MQ=255) gTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcgcactttg < 1:2519438‑M1/71‑55 (MQ=255) gTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTg > 1:1636809/1‑70 (MQ=255) gTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTg > 1:340662/1‑70 (MQ=255) tCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaat < 1:1711661/43‑1 (MQ=255) tCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaat < 1:1802677/43‑1 (MQ=255) tCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGa < 1:1035164/70‑1 (MQ=255) ttATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaataa > 1:3178201/1‑43 (MQ=255) tATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGa > 1:1527205/1‑62 (MQ=255) aTCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCa > 1:428038/1‑49 (MQ=255) aTCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGaaaa < 1:3833477/69‑1 (MQ=255) aTCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGaaaa < 1:3731410/69‑1 (MQ=255) aTCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGaaaa < 1:309547/69‑1 (MQ=255) cATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGAAAATTCt > 1:1575060/1‑71 (MQ=255) tGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGAAAATTCtt < 1:1095527/70‑1 (MQ=255) | GGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGAAAATTCTT > minE/222960‑223099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |