| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,211,250 | G→A | 24.2% | S34S (TCC→TCT) | cspC ← | stress protein, member of the CspA‑family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,211,250 | 0 | G | A | 24.2% | 182.5 / 57.1 | 99 | S34S (TCC→TCT) | cspC | stress protein, member of the CspA‑family |
| Reads supporting (aligned to +/- strand): ref base G (36/39); new base A (10/14); total (46/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
GCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTG > minE/1211181‑1211319 | gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:2478583/70‑1 (MQ=255) gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag > 1:1478149/1‑70 (MQ=255) gtgaTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAgtg < 1:477945/68‑1 (MQ=255) aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTAc > 1:1059989/1‑71 (MQ=255) ttCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa < 1:706655/68‑1 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGaa > 1:2571572/1‑62 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGaa > 1:156016/1‑62 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGa < 1:176980/70‑1 (MQ=255) cGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGaa > 1:1637821/1‑70 (MQ=255) aaCTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAacac > 1:372179/1‑71 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAacac < 1:24290/71‑1 (MQ=255) aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAacac < 1:3755859/70‑1 (MQ=255) aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAAcaca > 1:2657771/1‑71 (MQ=255) aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAAcaca > 1:3192446/1‑71 (MQ=255) cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:933161/52‑1 (MQ=255) cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAAcaca > 1:3527977/1‑70 (MQ=255) tCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGaa < 1:605378/56‑1 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGTAGAAGTGTACGAACACATCt < 1:2715024/70‑1 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt > 1:3407707/1‑71 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGAGTACGAACACATCt > 1:103504/1‑70 (MQ=255) aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAGGTGTACGAACACATCttt < 1:947240/71‑1 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACTCATCTTtg > 1:1761059/1‑71 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtg > 1:3923645/1‑71 (MQ=255) tttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAGTGTa < 1:2721220/55‑1 (MQ=255) gTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgc > 1:637407/1‑71 (MQ=255) ttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAGTGTACGAacactga < 1:621917/65‑4 (MQ=255) ttCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:2999891/45‑1 (MQ=255) ttCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:1544182/45‑1 (MQ=255) ttCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:1548692/45‑1 (MQ=255) cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg < 1:1901325/70‑1 (MQ=255) tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACAt > 1:2409280/1‑61 (MQ=255) gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa > 1:582459/1‑71 (MQ=255) aCCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa < 1:543206/70‑1 (MQ=255) aCCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGcc < 1:1559666/69‑1 (MQ=25) ccTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCAt > 1:3633317/1‑70 (MQ=255) cTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGaa < 1:3806519/52‑1 (MQ=255) cTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCAt > 1:1249309/1‑69 (MQ=255) tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca > 1:223531/1‑52 (MQ=255) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAac > 1:111609/1‑51 (MQ=255) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGcc > 1:440163/1‑65 (MQ=19) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTcttc > 1:3353712/1‑68 (MQ=16) aGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCg < 1:77269/71‑1 (MQ=255) aGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCg > 1:2785260/1‑71 (MQ=255) aGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCg < 1:1982487/71‑1 (MQ=255) gCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCgg < 1:1518876/71‑1 (MQ=255) ccAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGa < 1:559992/71‑1 (MQ=255) ccAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAGTGTACGAacact < 1:2276615/50‑2 (MQ=255) ccAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAGTGTACGAacact < 1:3563071/50‑2 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg > 1:1540361/1‑70 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt < 1:2122453/71‑1 (MQ=255) agTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTaa > 1:629527/1‑71 (MQ=255) agTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTaa > 1:2564493/1‑71 (MQ=255) ttttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg < 1:1300803/71‑1 (MQ=255) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg > 1:689997/1‑69 (MQ=255) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg > 1:3587239/1‑69 (MQ=255) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa < 1:3211159/70‑1 (MQ=255) gAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAg < 1:2114138/70‑1 (MQ=255) aaaccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAcc < 1:647470/68‑1 (MQ=12) aaccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTc < 1:103943/38‑1 (MQ=16) aaccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTc < 1:1407624/38‑1 (MQ=16) aaccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTtgctg > 1:2345410/3‑47 (MQ=255) aaccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAccg < 1:737923/69‑2 (MQ=255) aGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCGGCCGGAGTAATGAAGcc > 1:2443707/1‑70 (MQ=255) aGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGcc > 1:217784/1‑70 (MQ=255) gCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt < 1:2521295/41‑1 (MQ=255) gCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt < 1:3169696/60‑1 (MQ=255) accATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTct > 1:3407354/2‑51 (MQ=255) ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:2626606/71‑1 (MQ=255) cATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgct > 1:1345245/1‑43 (MQ=255) cATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:247712/70‑1 (MQ=255) ttACCCTGGATAGCGGAGAAGTGTACGATCACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaa < 1:2296236/67‑1 (MQ=255) ttACCCTGGATAGCGGAGAAGTGTACGAACACATCt < 1:3368449/36‑1 (MQ=255) ttACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa < 1:591721/45‑1 (MQ=255) aCCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgct < 1:1464705/39‑1 (MQ=255) aCCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgct < 1:1899480/39‑1 (MQ=255) aCCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATc > 1:1419261/1‑45 (MQ=255) aCCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCt < 1:3298623/70‑1 (MQ=255) cccTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:1499862/70‑1 (MQ=255) ccTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa < 1:1762428/71‑1 (MQ=255) cTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttg > 1:3132258/1‑69 (MQ=255) cTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCat > 1:2991813/1‑43 (MQ=12) cTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg < 1:3017016/44‑1 (MQ=255) tGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt < 1:1583846/67‑1 (MQ=255) tGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGc > 1:525164/1‑58 (MQ=12) tGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCttt < 1:3433160/68‑1 (MQ=12) tGGATAGCGGAGAAGTGTACGAACACATCTTTTCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:70980/62‑1 (MQ=255) tGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg < 1:3924194/54‑1 (MQ=255) ggATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:3065592/61‑1 (MQ=255) gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttgg < 1:3510652/68‑3 (MQ=255) gATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGAc < 1:2987128/70‑1 (MQ=255) gATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGAc < 1:831281/70‑1 (MQ=255) gATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGACGGAGTAATGAAGCCAAAACCTTTAGAc < 1:3585649/70‑1 (MQ=255) gATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGACGGAGTAATGAAGCCAAAACCTTTAGAc < 1:3581191/70‑1 (MQ=255) aTAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTc > 1:1876307/1‑71 (MQ=255) aTAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTc > 1:855873/1‑71 (MQ=255) gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:3017519/1‑58 (MQ=255) gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGtt > 1:74359/1‑71 (MQ=255) cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCttt > 1:2541735/1‑61 (MQ=255) cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTg < 1:2159560/71‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCgg < 1:309257/39‑1 (MQ=255) | GCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTG > minE/1211181‑1211319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |