| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,211,298 | G→A | 27.1% | G18G (GGC→GGT) | cspC ← | stress protein, member of the CspA‑family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,211,298 | 0 | G | A | 27.1% | 159.4 / 63.2 | 96 | G18G (GGC→GGT) | cspC | stress protein, member of the CspA‑family |
| Reads supporting (aligned to +/- strand): ref base G (30/40); new base A (15/11); total (45/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01 | |||||||||||
GAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGA > minE/1211229‑1211368 | gAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAg < 1:2114138/70‑1 (MQ=255) aaaccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAcc < 1:647470/68‑1 (MQ=12) aaccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAccg < 1:737923/69‑2 (MQ=255) aGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCGGCCGGAGTAATGAAGcc > 1:2443707/1‑70 (MQ=255) aGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGcc > 1:217784/1‑70 (MQ=255) ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:2626606/71‑1 (MQ=255) cATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:247712/70‑1 (MQ=255) ttACCCTGGATAGCGGAGAAGTGTACGATCACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaa < 1:2296236/67‑1 (MQ=255) aCCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCt < 1:3298623/70‑1 (MQ=255) cccTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:1499862/70‑1 (MQ=255) ccTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa < 1:1762428/71‑1 (MQ=255) cTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttg > 1:3132258/1‑69 (MQ=255) tGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt < 1:1583846/67‑1 (MQ=255) tGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGc > 1:525164/1‑58 (MQ=12) tGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCttt < 1:3433160/68‑1 (MQ=12) tGGATAGCGGAGAAGTGTACGAACACATCTTTTCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:70980/62‑1 (MQ=255) ggATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:3065592/61‑1 (MQ=255) gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttgg < 1:3510652/68‑3 (MQ=255) gATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGAc < 1:2987128/70‑1 (MQ=255) gATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGAc < 1:831281/70‑1 (MQ=255) gATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGACGGAGTAATGAAGCCAAAACCTTTAGAc < 1:3581191/70‑1 (MQ=255) gATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGACGGAGTAATGAAGCCAAAACCTTTAGAc < 1:3585649/70‑1 (MQ=255) aTAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTc > 1:855873/1‑71 (MQ=255) aTAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTc > 1:95075/1‑71 (MQ=255) aTAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTc > 1:1876307/1‑71 (MQ=255) gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:3017519/1‑58 (MQ=255) gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGtt > 1:74359/1‑71 (MQ=255) cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCttt > 1:3227927/1‑61 (MQ=255) cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCttt > 1:2541735/1‑61 (MQ=255) cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTg < 1:2159560/71‑1 (MQ=255) gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCgaat > 1:3068446/1‑53 (MQ=18) agaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAc > 1:2135013/1‑71 (MQ=255) agaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc > 1:1887077/1‑71 (MQ=255) agaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc > 1:3516745/1‑71 (MQ=255) agaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc > 1:3304559/1‑71 (MQ=255) gaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAcc < 1:699068/71‑1 (MQ=255) aGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCAc < 1:1681386/71‑1 (MQ=255) gtgtACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAccga > 1:685861/1‑45 (MQ=255) tgtACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt < 1:1521991/52‑1 (MQ=255) tgtACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACtt < 1:3909223/71‑1 (MQ=12) gtACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCTAAACCTTTAGACTCGTTGAACCACtt > 1:868425/1‑70 (MQ=255) gtACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACtt > 1:845793/1‑70 (MQ=255) tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTaaa > 1:578390/1‑63 (MQ=9) tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTa < 1:1194781/70‑1 (MQ=255) tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATACtttg > 1:883755/1‑51 (MQ=9) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:953471/50‑1 (MQ=255) aCGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAAc > 1:530704/1‑71 (MQ=255) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcg > 1:1793018/1‑70 (MQ=255) cGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCa > 1:676270/1‑41 (MQ=255) cGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:1429503/1‑45 (MQ=255) aaCACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGa > 1:1510217/1‑50 (MQ=255) acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:306497/1‑42 (MQ=255) acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTc < 1:272189/52‑1 (MQ=255) cacaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAc > 1:3287333/1‑71 (MQ=255) cacaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAc < 1:2904577/71‑1 (MQ=255) acaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCt < 1:2200473/67‑1 (MQ=255) aaaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAc > 1:263882/3‑70 (MQ=255) cgtCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCt > 1:1569698/3‑41 (MQ=12) gtCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCtt > 1:1462865/2‑71 (MQ=255) aTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt < 1:1820088/71‑1 (MQ=255) cTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCtt > 1:2838813/1‑69 (MQ=255) cTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGaa > 1:1231530/1‑52 (MQ=255) cTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCttt > 1:3855000/1‑70 (MQ=255) tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTa > 1:111644/1‑56 (MQ=255) tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTa > 1:3592555/1‑56 (MQ=255) tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATc > 1:1210538/1‑71 (MQ=255) tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATc > 1:2793454/1‑71 (MQ=255) tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATc > 1:2031568/1‑71 (MQ=255) ctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCtt < 1:1277899/71‑1 (MQ=255) ctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCtt < 1:83884/71‑1 (MQ=255) ctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCtt < 1:501487/71‑1 (MQ=255) tgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTg < 1:1698098/44‑1 (MQ=255) cATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGcc < 1:3587172/71‑1 (MQ=255) aTCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa > 1:2382057/1‑55 (MQ=255) aTCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCa < 1:3058124/71‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAcc < 1:690369/43‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAcc < 1:998981/43‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt < 1:1016430/71‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt < 1:2182732/71‑1 (MQ=255) ttccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtc > 1:2289156/3‑69 (MQ=255) aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAttt < 1:3669882/71‑1 (MQ=255) aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAttt > 1:2278971/1‑71 (MQ=255) aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt < 1:992184/70‑1 (MQ=255) tccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCtt < 1:2279505/54‑1 (MQ=16) gCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTg < 1:3357793/71‑1 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTaa < 1:1532320/57‑1 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTaa < 1:131685/57‑1 (MQ=255) gAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt < 1:1934341/64‑1 (MQ=255) aGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAc < 1:2600584/47‑1 (MQ=255) aGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa < 1:1373721/71‑1 (MQ=255) gTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGATAAAt > 1:2332611/1‑71 (MQ=255) tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTg < 1:174917/43‑1 (MQ=255) tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAAtt < 1:354049/71‑1 (MQ=255) tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAAt < 1:1242601/70‑1 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAttt > 1:3767160/1‑61 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc > 1:901757/1‑71 (MQ=255) aGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAg < 1:1061082/71‑1 (MQ=255) gCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGa < 1:1836371/71‑1 (MQ=255) | GAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGA > minE/1211229‑1211368 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |