| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,250,792 | A→C | 54.1% | L167L (CTT→CTG) | ruvC ← | component of RuvABC resolvasome, endonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,250,792 | 0 | A | C | 54.1% | 42.2 / 64.1 | 61 | L167L (CTT→CTG) | ruvC | component of RuvABC resolvasome, endonuclease |
| Reads supporting (aligned to +/- strand): ref base A (12/16); new base C (13/20); total (25/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCA > minE/1250725‑1250862 | gagggagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAg < 1:132145/70‑1 (MQ=255) gggagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGtt < 1:3376865/71‑1 (MQ=255) ggagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTc < 1:1267935/71‑1 (MQ=255) ggagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTc < 1:1546080/71‑1 (MQ=255) gagTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCa < 1:3439476/71‑1 (MQ=255) aaCGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAgg > 1:3366016/1‑60 (MQ=255) aaCGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGa > 1:897646/1‑71 (MQ=255) aCGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGAt > 1:704328/1‑71 (MQ=255) cGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGAtt < 1:3459006/71‑1 (MQ=255) cTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAg > 1:1404709/1‑54 (MQ=255) tCAGCCGGAAGTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCt > 1:1593720/1‑71 (MQ=255) tCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGAtt < 1:2524925/67‑1 (MQ=255) tCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCt > 1:949991/1‑71 (MQ=255) aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCa > 1:303735/1‑71 (MQ=255) aGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTc < 1:355047/70‑1 (MQ=255) gCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCAt > 1:284504/1‑71 (MQ=255) gCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCAt > 1:2718212/1‑71 (MQ=255) ccGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCa < 1:3232482/69‑1 (MQ=255) aaCTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCcgc < 1:271920/55‑1 (MQ=255) aaCTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCa > 1:2751237/1‑71 (MQ=255) aaCTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCa < 1:1568360/71‑1 (MQ=255) aaCTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCa > 1:2158845/1‑71 (MQ=255) aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCg < 1:2894932/69‑1 (MQ=255) aCCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCg < 1:236572/69‑1 (MQ=255) ccGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCa < 1:577593/70‑1 (MQ=255) cGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCAtt < 1:50235/71‑1 (MQ=255) aGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCt > 1:247452/1‑71 (MQ=255) gCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCa < 1:135771/60‑1 (MQ=255) gTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGAtt < 1:1624996/45‑1 (MQ=255) tataACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGAtt < 1:844813/44‑1 (MQ=255) tataACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCa < 1:304336/56‑1 (MQ=255) taACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGc < 1:732766/53‑1 (MQ=255) taACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAAc < 1:2096687/70‑1 (MQ=255) ttAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTaa < 1:2111905/65‑1 (MQ=255) ttAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTaa < 1:137700/65‑1 (MQ=255) ttAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTaa < 1:866174/65‑1 (MQ=255) ttAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTaa < 1:1497819/65‑1 (MQ=255) ttAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGt < 1:717332/68‑1 (MQ=255) tAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTgg > 1:1777447/1‑69 (MQ=255) tAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTgg > 1:3292912/1‑69 (MQ=255) aaCGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCt > 1:1141166/1‑57 (MQ=255) aaCGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACg > 1:1758371/1‑65 (MQ=255) aaCGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTg > 1:1792170/1‑67 (MQ=255) aaCGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGc < 1:2696166/69‑1 (MQ=255) aCGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGt < 1:3719878/71‑1 (MQ=255) cGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTg > 1:2908209/1‑71 (MQ=255) cGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTg > 1:3495484/1‑71 (MQ=255) cGCAGTCGCCCTCTCGCAAGGTTAAGCCGCGATTCGCTCATCTGCATAGCATTCTGACTAACGTGGCAGTg > 1:1143060/1‑71 (MQ=255) tCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCa < 1:1266173/41‑1 (MQ=255) tCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCa < 1:1924988/41‑1 (MQ=255) tCGCCCTCTCGCCAGCTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTTACTAACGTGGCAGTGGGTGa < 1:1116716/71‑1 (MQ=255) tCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAAc < 1:785167/57‑1 (MQ=255) tCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGa < 1:1402318/71‑1 (MQ=255) gCCCTCTCGCCAGGTTCAGCCGCGATTCGCTAATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGa > 1:1953268/1‑69 (MQ=255) ccTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAg > 1:1670295/1‑70 (MQ=255) ccTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGc < 1:1095557/71‑1 (MQ=255) ctctCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGc > 1:479333/1‑70 (MQ=255) tcGCCAGGTTCAGCCGCGATTTGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCaa < 1:2091331/69‑1 (MQ=255) tcGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATa > 1:1565399/1‑65 (MQ=255) gCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATc < 1:2645832/69‑1 (MQ=255) cAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGc > 1:3544342/1‑70 (MQ=255) aaGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGc > 1:1712060/1‑69 (MQ=255) aaGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGc > 1:851149/1‑69 (MQ=255) aaGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCa < 1:704963/71‑1 (MQ=255) | GAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCAAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGACTAACGTGGCAGTGGGTGATAGCAATCGCCA > minE/1250725‑1250862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |