| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,258,382 | A→T | 19.4% | intergenic (+53/+112) | yecP → / ← torZ | predicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,258,382 | 0 | A | T | 19.4% | 141.1 / 27.3 | 63 | intergenic (+53/+112) | yecP/torZ | predicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base A (24/24); new base T (5/7); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
ATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTA > minE/1258312‑1258449 | aTTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaaaa > 1:3021810/1‑71 (MQ=255) ttGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAc > 1:1390246/1‑71 (MQ=255) tGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCTGGCCTACAAAAcc > 1:308100/1‑71 (MQ=255) gcgcgcAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCt > 1:2389357/1‑71 (MQ=255) cgcgcAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCt < 1:3302930/70‑1 (MQ=255) cgcgcAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCt < 1:3626820/70‑1 (MQ=255) gcAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGc > 1:3292417/1‑70 (MQ=255) aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGGCTACAAAACCTTGCTAATTCAata > 1:2532500/1‑70 (MQ=255) aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAt > 1:1865022/1‑64 (MQ=255) aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAatat < 1:3380771/71‑1 (MQ=255) aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata > 1:745899/1‑70 (MQ=255) aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata > 1:2257478/1‑70 (MQ=255) ggTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAatatat < 1:1030772/71‑1 (MQ=255) gTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg > 1:3930305/1‑71 (MQ=255) cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTa < 1:3881990/70‑1 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGt < 1:3215171/70‑1 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTa < 1:2321149/71‑1 (MQ=255) ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAg > 1:2153568/1‑70 (MQ=255) gATGCGGCGTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGc < 1:1830028/71‑1 (MQ=2) aTGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGcc > 1:2502827/1‑71 (MQ=255) aTGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGcc > 1:1344960/1‑71 (MQ=255) gcggcgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCa < 1:40830/44‑1 (MQ=11) gcggcgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCa < 1:2575894/44‑1 (MQ=11) gcggcgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCa > 1:810565/1‑44 (MQ=255) gcggcgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCa > 1:1357186/1‑44 (MQ=255) ccggcgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCa < 1:1813584/43‑1 (MQ=255) cgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAc > 1:2948248/1‑55 (MQ=255) cgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGAt > 1:1902686/1‑69 (MQ=255) cgTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAAGCCTGAt > 1:1839011/1‑69 (MQ=2) gTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg < 1:2098586/71‑1 (MQ=255) tGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg < 1:28413/57‑1 (MQ=255) tGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGt < 1:2089834/58‑1 (MQ=255) gAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGATAAGcg > 1:846600/1‑70 (MQ=2) aaCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTgt < 1:2042568/45‑2 (MQ=11) aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAatatat < 1:138719/42‑1 (MQ=255) aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTAt > 1:3255345/1‑54 (MQ=255) aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTAt > 1:3302282/1‑54 (MQ=255) aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCa < 1:1560218/70‑1 (MQ=255) aCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTg < 1:1621388/43‑1 (MQ=14) aCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCa < 1:440427/45‑1 (MQ=255) aCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCa < 1:3812904/45‑1 (MQ=255) cGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAgc < 1:3361755/71‑1 (MQ=9) cGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAg > 1:3384363/1‑71 (MQ=255) ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg > 1:2996392/1‑71 (MQ=255) cTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg < 1:774345/43‑1 (MQ=255) ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg < 1:2185908/49‑1 (MQ=255) ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg < 1:2190403/49‑1 (MQ=255) aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCAt > 1:3007148/1‑69 (MQ=9) aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCAt > 1:2934480/1‑69 (MQ=9) aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCAt < 1:2422172/69‑1 (MQ=9) aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCAt < 1:1781149/69‑1 (MQ=9) aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg < 1:2323138/40‑1 (MQ=255) tCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt > 1:714290/1‑69 (MQ=255) ggCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGAGTAGCGCATCAGGct > 1:3661923/1‑70 (MQ=9) gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg < 1:2533442/42‑1 (MQ=255) gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg < 1:527739/42‑1 (MQ=255) gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAgg > 1:3797587/1‑69 (MQ=255) ccTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAg > 1:2191031/1‑71 (MQ=255) tACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg < 1:2401853/59‑1 (MQ=255) tACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg < 1:1502178/59‑1 (MQ=255) aCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg < 1:1940867/51‑1 (MQ=255) cAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAg > 1:3090375/1‑49 (MQ=9) cAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAg > 1:58022/1‑49 (MQ=9) cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCa > 1:2874553/1‑62 (MQ=255) aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTa > 1:3594583/1‑71 (MQ=255) aaaCCTTGCTAATTCAATATATTGCAGGGACTATg < 1:1095669/35‑1 (MQ=255) | ATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTA > minE/1258312‑1258449 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |