| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,258,429 | A→G | 9.5% | intergenic (+100/+65) | yecP → / ← torZ | predicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,258,429 | 0 | A | G | 9.5% | 163.7 / 7.6 | 63 | intergenic (+100/+65) | yecP/torZ | predicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base A (29/28); new base G (3/3); total (32/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCACGTCTTAC > minE/1258360‑1258497 | aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCa < 1:1560218/70‑1 (MQ=255) cGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAgc < 1:3361755/71‑1 (MQ=9) cGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAg > 1:3384363/1‑71 (MQ=255) ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg > 1:2996392/1‑71 (MQ=255) aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCAt < 1:2422172/69‑1 (MQ=9) aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCAt > 1:2934480/1‑69 (MQ=9) aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCAt > 1:3007148/1‑69 (MQ=9) aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCAt < 1:1781149/69‑1 (MQ=9) tCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt > 1:714290/1‑69 (MQ=255) ggCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGAGTAGCGCATCAGGct > 1:3661923/1‑70 (MQ=9) gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAgg > 1:3797587/1‑69 (MQ=255) ccTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAg > 1:2191031/1‑71 (MQ=255) tACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg < 1:1502178/59‑1 (MQ=255) tACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg < 1:2401853/59‑1 (MQ=255) cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCa > 1:2874553/1‑62 (MQ=255) aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTa > 1:3594583/1‑71 (MQ=255) ccTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGtt > 1:2534001/1‑71 (MQ=255) gCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGttt > 1:3749742/1‑68 (MQ=255) gCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGc < 1:2375998/70‑1 (MQ=255) cTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcgc > 1:1255422/1‑48 (MQ=255) cTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt < 1:3446304/71‑1 (MQ=255) aTTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATa < 1:54899/69‑1 (MQ=255) aTTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATa < 1:2988513/69‑1 (MQ=255) aTTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAAc < 1:3152461/71‑1 (MQ=255) aTTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAAc > 1:791146/1‑71 (MQ=255) ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCAt < 1:287292/39‑1 (MQ=9) ttCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATa > 1:594385/1‑68 (MQ=255) ttCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAAcc < 1:1051038/71‑1 (MQ=255) ttCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAAcc > 1:3091160/1‑71 (MQ=255) tCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCtt > 1:3411357/1‑55 (MQ=255) cAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGt > 1:1087146/1‑59 (MQ=9) cAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCt < 1:3874387/70‑1 (MQ=255) cAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCt < 1:3869756/70‑1 (MQ=255) cAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCt > 1:1664035/1‑70 (MQ=255) atatatTGCAGGGACTATGTAGGCCTGATAAGCATAgcg < 1:2482192/39‑1 (MQ=255) tatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCttt > 1:199104/1‑52 (MQ=255) tatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCt > 1:1520544/1‑50 (MQ=255) ttGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGccc > 1:874143/1‑71 (MQ=255) ttGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGccc < 1:453880/71‑1 (MQ=255) tGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGccc > 1:2095191/1‑70 (MQ=255) cAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGt < 1:944924/70‑1 (MQ=255) cAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGt < 1:3854815/70‑1 (MQ=255) aGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGttt < 1:3293059/71‑1 (MQ=255) ggACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACg < 1:3300766/45‑1 (MQ=255) ggACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTcc < 1:1931014/71‑1 (MQ=255) ggACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTcc < 1:3560809/71‑1 (MQ=255) aTGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAAc > 1:2049194/1‑50 (MQ=255) aTGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAAc > 1:1884088/1‑50 (MQ=255) tGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAgcgc > 1:791239/1‑57 (MQ=255) tGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAgcgc > 1:1514191/1‑57 (MQ=255) gTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGTTTgt < 1:3617267/43‑2 (MQ=11) gTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGTTTgt < 1:2320350/43‑2 (MQ=11) gTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGTTTgt < 1:409011/43‑2 (MQ=11) ggCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTAt < 1:1309310/70‑1 (MQ=255) accTGATAAGCATAGCGCATCAGGCTGCTTTGCGtt < 1:21456/35‑1 (MQ=14) cTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAgc > 1:3824594/1‑48 (MQ=255) cTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGttt < 1:2460936/56‑1 (MQ=255) cTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCAc > 1:2537592/1‑71 (MQ=255) tGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCAc > 1:1352657/1‑70 (MQ=255) gATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTcc > 1:1925341/1‑56 (MQ=255) aTAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTTAGCGCCCGTTTCCGGGCGCTATTCACGTc > 1:2022117/1‑71 (MQ=255) aGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCACGTCtt < 1:3014099/70‑1 (MQ=255) gCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGgcg < 1:3347696/56‑1 (MQ=255) cATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCACGTCTTAc < 1:52087/70‑1 (MQ=255) aTAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAg > 1:1115070/1‑38 (MQ=255) | AACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGCTATTCACGTCTTAC > minE/1258360‑1258497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |