Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,259,358 G→A 17.8% N522N (AAC→AAT torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,259,3580GA17.8% 234.1 / 39.1 101N522N (AAC→AATtorZtrimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (44/39);  new base A (14/4);  total (58/43)
Fisher's exact test for biased strand distribution p-value = 6.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01

Only 100 of 105 total aligned reads displayed.

ACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCT  >  minE/1259291‑1259427
                                                                   |                                                                     
acacGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTg                                                                    <  1:2929217/71‑1 (MQ=255)
 cacGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGc                                                                   <  1:544249/71‑1 (MQ=255)
    gTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCt                                                                  >  1:2098813/1‑69 (MQ=255)
    gTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTg                                                                 >  1:3067295/1‑70 (MQ=255)
    gTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGt                                                                <  1:504385/71‑1 (MQ=255)
    atCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGt                                                                <  1:1807957/70‑1 (MQ=255)
     tCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTa                                                               <  1:188086/71‑1 (MQ=255)
       aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAAt                                                             <  1:2587043/71‑1 (MQ=255)
       aaaaTCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTAAt                                                             <  1:337808/71‑1 (MQ=255)
        aaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAAt                                                             >  1:1249524/1‑70 (MQ=255)
          aTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCAc                                                          <  1:1778016/71‑1 (MQ=255)
          aTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCAc                                                          <  1:470536/71‑1 (MQ=255)
           tCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCAcc                                                         >  1:3105599/1‑71 (MQ=255)
           tCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCAcc                                                         >  1:513725/1‑71 (MQ=255)
           tCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCAcc                                                         >  1:3372617/1‑71 (MQ=255)
            cGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCg                                                        >  1:151080/1‑71 (MQ=255)
            cGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCg                                                        >  1:3768493/1‑71 (MQ=255)
            cGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCg                                                        >  1:3200162/1‑71 (MQ=255)
             gTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCgg                                                       >  1:455261/1‑71 (MQ=255)
             gTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCg                                                        >  1:128098/1‑70 (MQ=255)
             gTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCg                                                        >  1:746990/1‑70 (MQ=255)
                aCGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTc                                                     <  1:2202681/70‑1 (MQ=255)
                 cgcgCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCAt                                                   <  1:1215971/71‑1 (MQ=255)
                  gcgcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc                                                  >  1:3400514/1‑71 (MQ=255)
                    gcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGt                                                >  1:1666713/1‑71 (MQ=255)
                    gcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGt                                                >  1:3452958/1‑71 (MQ=255)
                     cTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATc                                                            <  1:397559/58‑1 (MQ=255)
                       tCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGc                                                                   >  1:1738389/1‑49 (MQ=255)
                         aaaTTGCTGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAg                                            <  1:921377/70‑1 (MQ=255)
                          aaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAg                                            >  1:1433139/1‑69 (MQ=255)
                           aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                                         <  1:1517939/71‑1 (MQ=255)
                               cGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTgc                                     >  1:3544148/1‑71 (MQ=255)
                                  aGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCt                                  >  1:3029568/1‑71 (MQ=255)
                                  aGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCt                                  >  1:2405667/1‑71 (MQ=255)
                                     aaCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCa                                                    <  1:2219828/50‑1 (MQ=255)
                                     aaCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                                         <  1:2682738/61‑1 (MQ=255)
                                      aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAt                                        <  1:1403782/61‑1 (MQ=255)
                                       cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc                             >  1:231363/1‑71 (MQ=255)
                                       cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATAACCGGTCATAGTCAAGTCATTGCGCTCaaa                              >  1:531696/1‑70 (MQ=255)
                                       cAGCCTGCTTCATCGGCACAATATCCTGGTTGCTGTAATCAc                                                          >  1:791073/1‑42 (MQ=255)
                                         gCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGa                           <  1:926415/71‑1 (MQ=255)
                                         gCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGATCAAACGa                           <  1:828043/71‑1 (MQ=255)
                                           cTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg                         <  1:2783061/71‑1 (MQ=255)
                                           cTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg                         <  1:3891486/71‑1 (MQ=255)
                                           cTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg                         <  1:3004255/71‑1 (MQ=255)
                                            tGCTTCATCGGCACCAGATGCTGATTACTGTAATCa                                                           >  1:2650871/1‑36 (MQ=19)
                                            tGCTTCATCGGCACCAGATGCTGATTACTGTAATCa                                                           >  1:454530/1‑36 (MQ=19)
                                            tGCTTCATCGGCACCAGATGCTGATTACTGTAATCa                                                           >  1:3555863/1‑36 (MQ=19)
                                            tGCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGagg                         >  1:3640263/1‑68 (MQ=255)
                                            tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGTTCATCGTCAAGTCATTGCGCTCAAACGAtgt                        >  1:3010057/1‑71 (MQ=255)
                                            tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGt                                                      <  1:1314366/41‑1 (MQ=255)
                                            tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc                                                  <  1:1062837/45‑1 (MQ=255)
                                            tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc                                                  <  1:2943726/45‑1 (MQ=255)
                                             gCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCAtgg                                                 >  1:3190134/1‑43 (MQ=255)
                                             gCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGagg                         >  1:1256085/1‑67 (MQ=255)
                                             gCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                       >  1:3578712/1‑71 (MQ=255)
                                              cTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAggtag                      >  1:2750524/1‑69 (MQ=255)
                                              cTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAggtag                      <  1:2453958/71‑3 (MQ=255)
                                              cTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTgc                                     <  1:1701252/56‑1 (MQ=255)
                                              cTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                       <  1:1514050/70‑1 (MQ=255)
                                               ttCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGgtagt                     >  1:1730518/1‑71 (MQ=255)
                                               ttCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc                                                  >  1:771381/1‑42 (MQ=255)
                                               ttCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc                                                  >  1:282319/1‑42 (MQ=255)
                                                tCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCaaa                              >  1:1632711/1‑61 (MQ=255)
                                                 cATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGgtagtcg                   >  1:2029917/1‑69 (MQ=255)
                                                 cATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaa                               <  1:1577647/59‑1 (MQ=255)
                                                 cATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaa                               <  1:3494622/59‑1 (MQ=255)
                                                 cATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtggtg                    <  1:3951101/70‑1 (MQ=255)
                                                  aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAt                                        >  1:2068752/1‑49 (MQ=255)
                                                  aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                  >  1:1764250/1‑71 (MQ=255)
                                                  aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                  >  1:2901012/1‑71 (MQ=255)
                                                  aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                  <  1:3708710/71‑1 (MQ=255)
                                                  aTCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                  >  1:3490521/1‑71 (MQ=255)
                                                   tCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg                         <  1:3646008/63‑1 (MQ=255)
                                                   tCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtggtg                    <  1:851301/68‑1 (MQ=255)
                                                    cGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTAAAACGATGTGGTGATCg                >  1:1511245/1‑71 (MQ=255)
                                                     ggCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAggtag                      >  1:888718/1‑62 (MQ=255)
                                                     ggCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAggtag                      >  1:1710461/1‑62 (MQ=255)
                                                     ggCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCgg               >  1:1728536/1‑71 (MQ=255)
                                                      gCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCa                                <  1:1157681/53‑1 (MQ=255)
                                                      gCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCa                                <  1:1309532/53‑1 (MQ=255)
                                                      gCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGt              <  1:2305141/71‑1 (MQ=255)
                                                      gCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGt              <  1:440249/71‑1 (MQ=255)
                                                        acaATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa                              >  1:1359624/1‑53 (MQ=255)
                                                        acaATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa                              >  1:1563468/1‑53 (MQ=255)
                                                        acaATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTaa            <  1:3484133/71‑1 (MQ=255)
                                                        acaATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTaa            >  1:2131078/1‑71 (MQ=255)
                                                        acaATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTa             >  1:2015190/1‑70 (MQ=255)
                                                        acaATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCCCTCAAACGATGTGGTGATCGGTaa            <  1:3065910/71‑1 (MQ=255)
                                                         caATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTaa            >  1:879018/1‑70 (MQ=255)
                                                           atatGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATAc         >  1:1187059/1‑71 (MQ=255)
                                                              tGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc                             >  1:654891/1‑48 (MQ=255)
                                                              tGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc                             >  1:554875/1‑48 (MQ=255)
                                                              tGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGat      <  1:3498616/71‑1 (MQ=255)
                                                              tGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGa       <  1:1297889/70‑1 (MQ=255)
                                                                 tGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATc   >  1:3649842/1‑71 (MQ=255)
                                                                  ggTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACg                            <  1:2851295/45‑1 (MQ=255)
                                                                  ggTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATAc         <  1:1706173/64‑1 (MQ=255)
                                                                   gTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCt  <  1:670249/70‑1 (MQ=255)
                                                                   gTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCt  <  1:3651623/70‑1 (MQ=255)
                                                                   |                                                                     
ACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCT  >  minE/1259291‑1259427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: