Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,259,859 | A→C | 20.3% | I355M (ATT→ATG) | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,259,859 | 0 | A | C | 20.3% | 104.0 / 32.4 | 64 | I355M (ATT→ATG) | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
Reads supporting (aligned to +/- strand): ref base A (17/34); new base C (9/4); total (26/38) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.72e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGACTATCTGCGCT > minE/1259791‑1259928 | atttGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCAt > 1:1223111/4‑71 (MQ=255) ccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa < 1:843594/71‑1 (MQ=255) ccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa < 1:3514365/71‑1 (MQ=255) caaCATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGc > 1:3033525/2‑71 (MQ=255) aCATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCt > 1:2520160/1‑71 (MQ=255) cATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTg < 1:2655954/71‑1 (MQ=255) gctgccAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCa < 1:3094874/67‑1 (MQ=255) gccgccAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt > 1:2221078/1‑71 (MQ=255) gccgccAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt > 1:2216343/1‑71 (MQ=255) ctgccAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCAt > 1:3379490/3‑70 (MQ=255) ctgccAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCAt < 1:2241161/68‑1 (MQ=255) gccAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCATCAg > 1:1137738/1‑71 (MQ=255) gccAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAg > 1:2827844/1‑71 (MQ=255) ccAGCCTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTg < 1:1938514/63‑1 (MQ=255) cAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCa < 1:462589/71‑1 (MQ=255) cAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCa > 1:3621450/1‑71 (MQ=255) cAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCa < 1:3841247/71‑1 (MQ=255) cAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCa < 1:235307/71‑1 (MQ=255) cAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCa < 1:1507521/71‑1 (MQ=255) cAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCa < 1:1910719/71‑1 (MQ=255) aGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAt > 1:792378/1‑71 (MQ=255) gCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCATCAGCAtg < 1:480872/71‑2 (MQ=255) gCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCATCAGCAtg < 1:1881162/71‑2 (MQ=255) cGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATtg > 1:1084140/1‑71 (MQ=255) gTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATtgt < 1:415203/71‑1 (MQ=255) gTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATtg < 1:371696/70‑1 (MQ=255) cACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGc > 1:2726561/1‑71 (MQ=255) aGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGAtt > 1:3160833/1‑71 (MQ=255) gCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTc < 1:2928067/71‑1 (MQ=255) gCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTc > 1:3191134/1‑71 (MQ=255) aTCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTcgc < 1:802895/71‑1 (MQ=255) tCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTcgcc < 1:715872/71‑1 (MQ=255) cAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCATc > 1:1671019/1‑51 (MQ=255) cAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCATc > 1:3445555/1‑51 (MQ=255) cAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCATc > 1:1226739/1‑51 (MQ=255) cAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCATc > 1:1240331/1‑51 (MQ=255) gtgtTTCTGTTCACCATATTGCTGGCGCTGAATTcccc < 1:352911/38‑1 (MQ=255) gtgtTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGAt < 1:1949623/62‑1 (MQ=255) tgtTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTc < 1:3684044/63‑1 (MQ=255) tgtTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCAt < 1:1407074/71‑1 (MQ=255) tgtTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCAt < 1:1126505/71‑1 (MQ=255) gtTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGGCATCAGCATTGTGCGATTCGCCGCCAt > 1:34580/1‑70 (MQ=255) gtTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGc < 1:494234/57‑1 (MQ=255) tttCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTcgccgc < 1:295695/66‑1 (MQ=255) tttCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCgccgcc < 1:3369877/67‑1 (MQ=255) tttCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATc < 1:1356276/70‑1 (MQ=255) tttCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATc < 1:3370159/70‑1 (MQ=255) tCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAg < 1:57242/70‑1 (MQ=255) cTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGtt > 1:310941/1‑71 (MQ=255) cTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGtt > 1:3924097/1‑71 (MQ=255) cACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGc < 1:3130496/70‑1 (MQ=255) aCCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGa < 1:2943778/71‑1 (MQ=255) ccATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAg < 1:2178134/71‑1 (MQ=255) cATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGt > 1:3100847/1‑71 (MQ=255) tatTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGttt < 1:3767300/71‑1 (MQ=255) tGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCAt > 1:1236801/1‑53 (MQ=255) tGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCAACAGTTCAGCGAGTTTGAc > 1:1866554/1‑71 (MQ=255) gCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGAc < 1:780591/70‑1 (MQ=255) gcgcTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCTCCGCCATCAGTTCAGCGAGTTTGACTATCt < 1:816315/71‑1 (MQ=255) gcgcTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGACTAt < 1:3090433/69‑1 (MQ=255) gcTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGACTATCTgc < 1:1413161/71‑1 (MQ=255) cTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGACTATCTgcg < 1:1911283/71‑1 (MQ=255) gAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGa > 1:2623334/1‑60 (MQ=255) gAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGACTATCTGCGCt > 1:720879/1‑71 (MQ=255) aaTTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGc < 1:842486/51‑1 (MQ=255) aaTTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAg < 1:3915133/54‑1 (MQ=255) | ATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGACTATCTGCGCT > minE/1259791‑1259928 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |