Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 63,058 G→A 7.4% T134I (ACT→ATT)  polB ← DNA polymerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE63,0580GA 7.4% 160.3 / 3.1 54T134I (ACT→ATT) polBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (33/17);  new base A (2/2);  total (35/19)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTG  >  minE/62996‑63127
                                                              |                                                                     
taGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCa                                                                  <  1:640950/68‑1 (MQ=255)
taGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATtg                                                               <  1:3915707/71‑1 (MQ=255)
taGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATtg                                                               <  1:1257820/71‑1 (MQ=255)
  gAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATtgt                                                              <  1:34236/70‑1 (MQ=255)
    aaCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCa                                                           <  1:1748270/71‑1 (MQ=255)
     aCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCat                                                          <  1:3799938/71‑1 (MQ=255)
     aCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCa                                                           >  1:2907924/1‑70 (MQ=255)
     aCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCa                                                           >  1:1490486/1‑70 (MQ=255)
       ccACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCatat                                                        <  1:1234864/71‑1 (MQ=255)
         aCTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCa                                                      >  1:2055238/1‑71 (MQ=255)
            tGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCAcc                                                    >  1:2833113/1‑70 (MQ=255)
                  gcggACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGAcc                                             >  1:3209140/1‑71 (MQ=255)
                        gATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACAcc                                       <  1:384981/71‑1 (MQ=255)
                           aGTCGGGATGCGGTTTCAGACGGGCATTAACGATAg                                                                       <  1:3559714/36‑1 (MQ=255)
                           aGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCAcc                                                    >  1:2832398/1‑55 (MQ=255)
                           aGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggt                                    >  1:2264294/1‑71 (MQ=255)
                            gTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggt                                    >  1:3730974/1‑70 (MQ=255)
                             tCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtga                                  <  1:945813/71‑1 (MQ=255)
                              cGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgag                                 >  1:3543699/1‑71 (MQ=255)
                               gggATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgagg                                >  1:2835111/1‑71 (MQ=255)
                               gggATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgagg                                >  1:1573966/1‑71 (MQ=255)
                                     cGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCata                                                         >  1:1244860/1‑40 (MQ=255)
                                     cGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCata                                                         >  1:892390/1‑40 (MQ=255)
                                     cGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCata                                                         >  1:3665811/1‑40 (MQ=255)
                                     cGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATa                           >  1:3241377/1‑70 (MQ=255)
                                      ggTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCca                                           >  1:386437/1‑53 (MQ=255)
                                      ggTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATaaa                         >  1:2351909/1‑71 (MQ=255)
                                          tCAGACGGGGATTAACGATAGTGCCATTGTGCATATCACCCTc                                                 >  1:2619606/1‑43 (MQ=255)
                                          tCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGc                     >  1:2082603/1‑71 (MQ=255)
                                           cAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCt                    >  1:328870/1‑71 (MQ=255)
                                            aGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTc                   >  1:2606051/1‑71 (MQ=255)
                                              aCGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCa                 <  1:215644/71‑1 (MQ=255)
                                               cGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt                <  1:729795/71‑1 (MQ=255)
                                               atggCACTATCGTTAATGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt                <  1:3883913/69‑1 (MQ=38)
                                               atggCACTATCGTTAATGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt                <  1:697070/69‑1 (MQ=38)
                                                tggCACTATCGTTAATGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt                >  1:1814764/2‑70 (MQ=255)
                                                tggCACTATCGTTAATGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt                >  1:1882755/2‑70 (MQ=255)
                                                   cATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt                >  1:708358/1‑67 (MQ=255)
                                                   cATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGa            <  1:2852539/71‑1 (MQ=255)
                                                   cATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGa            <  1:1354054/71‑1 (MQ=255)
                                                    aTTAACGATAGTGCCATTGTGCATATCACCCTCGCCCCACACCGGTGAGGTGATAAACCGCTCCATCAGa            >  1:1959647/1‑70 (MQ=255)
                                                    aTTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt                >  1:2826842/1‑66 (MQ=255)
                                                     ttAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGa            >  1:1030964/1‑69 (MQ=255)
                                                     ttAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGa            >  1:685845/1‑69 (MQ=255)
                                                      tAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAAc                        >  1:3456679/1‑56 (MQ=255)
                                                      tAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAg         >  1:1234885/1‑71 (MQ=255)
                                                       aaCGATAGTGCCATTGTGCATATCACCCTCGACCcaca                                         >  1:1384704/1‑38 (MQ=255)
                                                       aaCGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgag                                 <  1:1403659/46‑1 (MQ=255)
                                                       aaCGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgag                                 >  1:3372039/1‑46 (MQ=255)
                                                       aaCGATAGTGCCATTGTGCATATCACCCTCGACCCACACCg                                      >  1:412935/1‑41 (MQ=255)
                                                        aCGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAAcc                       <  1:3712400/55‑1 (MQ=255)
                                                        aCGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAAcc                       <  1:1003400/55‑1 (MQ=255)
                                                         cGATAGTGCCATTGTGCATATCACCCTCGACCCACACCggtgaggt                               >  1:1442464/1‑46 (MQ=255)
                                                            tAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCt   <  1:2970537/71‑1 (MQ=255)
                                                            tAGTGCCATTGTGCATATCACCATCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGa            >  1:1034844/1‑62 (MQ=255)
                                                              gTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTg  <  1:2239585/70‑1 (MQ=255)
                                                              |                                                                     
TAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTG  >  minE/62996‑63127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑