| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,260,306 | G→A | 7.8% | N206N (AAC→AAT) ‡ | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,260,306 | 0 | G | A | 7.8% | 189.5 / 5.0 | 64 | N206N (AAC→AAT) ‡ | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base G (29/30); new base A (3/2); total (32/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01 | |||||||||||
ATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGA > minE/1260237‑1260376 | aTCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGt < 1:1148274/71‑1 (MQ=255)aTCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGt < 1:76736/71‑1 (MQ=255)aTCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGt < 1:3346383/71‑1 (MQ=255)aTCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGt > 1:1923883/1‑71 (MQ=255) tCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGtt < 1:3332877/71‑1 (MQ=255) cGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGttt > 1:2900297/1‑71 (MQ=255) gTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGt < 1:353791/67‑1 (MQ=255) gTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTc > 1:454933/1‑71 (MQ=255) aCTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTc > 1:3041054/1‑69 (MQ=255) cTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAAt > 1:2222404/1‑71 (MQ=255) cAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTc < 1:2070739/62‑1 (MQ=255) cAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAg < 1:3273270/71‑1 (MQ=255) cAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAg < 1:2138349/71‑1 (MQ=255) gCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTc < 1:546595/59‑1 (MQ=255) gCAATTTTCAGCGTATTGAGTGGGTTAGCACTCCACAGCACCACGACATCGCTATGTTCCAGCACCAGCgg > 1:1259526/1‑71 (MQ=255) aaTTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCa > 1:3121765/1‑59 (MQ=255) aaTTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGc > 1:625060/1‑70 (MQ=255) ttttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCag < 1:619757/70‑1 (MQ=255) tttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg < 1:3096416/70‑1 (MQ=255) tttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCag > 1:437424/1‑69 (MQ=255) ttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggag > 1:187155/1‑71 (MQ=255) aaGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggagg < 1:2070520/70‑1 (MQ=255) ggTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCa < 1:1393863/50‑1 (MQ=255) ggTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCa < 1:667998/50‑1 (MQ=255) ggTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTct > 1:131527/1‑71 (MQ=255) gTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTc < 1:2303606/69‑1 (MQ=255) cAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGtt > 1:3410339/1‑71 (MQ=255) cAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGt < 1:3639974/70‑1 (MQ=255) aGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGtt > 1:3742534/1‑70 (MQ=255) gCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa < 1:2796313/71‑1 (MQ=255) cGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa < 1:1812133/70‑1 (MQ=255) cGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa > 1:1677033/1‑70 (MQ=255) gTTCATTGCCCACAGCACCACAACCTGGCTGTTTTCCAGAAt < 1:449612/42‑1 (MQ=255) gTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg < 1:1619114/53‑1 (MQ=255) ttCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgctg > 1:990780/1‑62 (MQ=255) tCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACAcc > 1:1263240/1‑71 (MQ=255) cATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACTCct > 1:129980/1‑71 (MQ=255) ttCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg < 1:461562/48‑1 (MQ=255) ttCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg < 1:895122/48‑1 (MQ=255) tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa > 1:1426793/1‑71 (MQ=255) tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa > 1:2105493/1‑71 (MQ=255) tCCCCACAGCACCACAACCTGCCTGTTTTCCAGAATCACCGGCCAGGAGGTCTGCTGTTCATACACCTCTa > 1:2720775/1‑71 (MQ=255) ccccACAGCACCACAACCTGGCTGTTTTCCAGAATCAg > 1:2683092/1‑38 (MQ=255) ccccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa < 1:871553/70‑1 (MQ=255) ccccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa < 1:1941037/70‑1 (MQ=255) ccccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa < 1:3739683/70‑1 (MQ=255) tccACAGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaa < 1:3656911/62‑3 (MQ=255) tccACAGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACttc < 1:3639044/66‑1 (MQ=255) ccACAGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaactt > 1:388237/1‑63 (MQ=255) cacaGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACttcac > 1:472205/1‑65 (MQ=255) cacaGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGt > 1:2882729/1‑54 (MQ=255) cacaGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg > 1:223710/1‑70 (MQ=255) cacaGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg > 1:2913386/1‑70 (MQ=255) cacaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgc > 1:945875/1‑43 (MQ=255) caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgc > 1:1539500/1‑41 (MQ=255) caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATa > 1:1245523/1‑49 (MQ=255) caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATa > 1:2285047/1‑49 (MQ=255) caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca > 1:2305958/1‑71 (MQ=255) caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca > 1:2309483/1‑71 (MQ=255) aaCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACAt < 1:3347492/71‑1 (MQ=255) aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATg < 1:1341823/71‑1 (MQ=255) gCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACca < 1:1348772/62‑1 (MQ=255) gCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCgg < 1:714210/69‑1 (MQ=255) cTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCAt < 1:962572/71‑1 (MQ=255) gTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGa > 1:1690957/1‑71 (MQ=255) | ATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGA > minE/1260237‑1260376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |