Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,260,315 A→C 8.6% I203M (ATT→ATG) ‡ torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,260,3150AC 8.6% 138.4 / 8.4 58I203M (ATT→ATG) ‡torZtrimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base A (29/24);  new base C (3/2);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

CTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTG  >  minE/1260247‑1260383
                                                                    |                                                                    
cTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAAt                                                                    >  1:2222404/1‑71 (MQ=255)
   cAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAg                                                                 <  1:3273270/71‑1 (MQ=255)
   cAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAg                                                                 <  1:2138349/71‑1 (MQ=255)
      gCAATTTTCAGCGTATTGAGTGGGTTAGCACTCCACAGCACCACGACATCGCTATGTTCCAGCACCAGCgg                                                              >  1:1259526/1‑71 (MQ=255)
        aaTTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGc                                                             >  1:625060/1‑70 (MQ=255)
           ttttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCag                                                          <  1:619757/70‑1 (MQ=255)
            tttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg                                                         <  1:3096416/70‑1 (MQ=255)
            tttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCag                                                          >  1:437424/1‑69 (MQ=255)
             ttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggag                                                       >  1:187155/1‑71 (MQ=255)
               aaGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggagg                                                      <  1:2070520/70‑1 (MQ=255)
                 ggTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTct                                                   >  1:131527/1‑71 (MQ=255)
                  gTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTc                                                    <  1:2303606/69‑1 (MQ=255)
                       cAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGtt                                             >  1:3410339/1‑71 (MQ=255)
                       cAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGt                                              <  1:3639974/70‑1 (MQ=255)
                        aGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGtt                                             >  1:3742534/1‑70 (MQ=255)
                         gCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa                                           <  1:2796313/71‑1 (MQ=255)
                          cGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa                                           <  1:1812133/70‑1 (MQ=255)
                          cGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa                                           >  1:1677033/1‑70 (MQ=255)
                             gTTCATTGCCCACAGCACCACAACCTGGCTGTTTTCCAGAAt                                                                    <  1:449612/42‑1 (MQ=255)
                             gTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg                                                         <  1:1619114/53‑1 (MQ=255)
                              ttCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgctg                                               >  1:990780/1‑62 (MQ=255)
                               tCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACAcc                                     >  1:1263240/1‑71 (MQ=255)
                                cATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACTCct                                    >  1:129980/1‑71 (MQ=255)
                                  ttCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg                                                         <  1:895122/48‑1 (MQ=255)
                                  ttCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg                                                         <  1:461562/48‑1 (MQ=255)
                                   tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa                                 >  1:2105493/1‑71 (MQ=255)
                                   tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa                                 >  1:1426793/1‑71 (MQ=255)
                                   tCCCCACAGCACCACAACCTGCCTGTTTTCCAGAATCACCGGCCAGGAGGTCTGCTGTTCATACACCTCTa                                 >  1:2720775/1‑71 (MQ=255)
                                    ccccACAGCACCACAACCTGGCTGTTTTCCAGAATCAg                                                                 >  1:2683092/1‑38 (MQ=255)
                                    ccccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa                                 <  1:1941037/70‑1 (MQ=255)
                                    ccccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa                                 <  1:871553/70‑1 (MQ=255)
                                    ccccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa                                 <  1:3739683/70‑1 (MQ=255)
                                     tccACAGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaa                                       <  1:3656911/62‑3 (MQ=255)
                                     tccACAGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACttc                                   <  1:3639044/66‑1 (MQ=255)
                                      ccACAGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaactt                                    >  1:388237/1‑63 (MQ=255)
                                       cacaGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACttcac                                 >  1:472205/1‑65 (MQ=255)
                                       cacaGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGt                                              >  1:2882729/1‑54 (MQ=255)
                                       cacaGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg                              >  1:223710/1‑70 (MQ=255)
                                       cacaGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg                              >  1:2913386/1‑70 (MQ=255)
                                               cacaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgc                                                 >  1:945875/1‑43 (MQ=255)
                                                 caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgc                                                 >  1:1539500/1‑41 (MQ=255)
                                                 caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATa                                         >  1:1245523/1‑49 (MQ=255)
                                                 caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATa                                         >  1:2285047/1‑49 (MQ=255)
                                                 caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca                   >  1:2305958/1‑71 (MQ=255)
                                                 caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca                   >  1:2309483/1‑71 (MQ=255)
                                                  aaCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACAt                  <  1:3347492/71‑1 (MQ=255)
                                                   aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATg                 <  1:1341823/71‑1 (MQ=255)
                                                        gCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACca                     <  1:1348772/62‑1 (MQ=255)
                                                        gCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCgg              <  1:714210/69‑1 (MQ=255)
                                                         cTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCAt           <  1:962572/71‑1 (MQ=255)
                                                           gTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGa         >  1:1690957/1‑71 (MQ=255)
                                                            ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGa                        >  1:1892699/1‑55 (MQ=255)
                                                            ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg          <  1:818390/69‑1 (MQ=255)
                                                            ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg          <  1:2942994/69‑1 (MQ=255)
                                                              ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACAGACCACATGCGGCATGATCa      >  1:3198086/1‑71 (MQ=255)
                                                                  aGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtg  <  1:866807/71‑1 (MQ=255)
                                                                   gAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGc                >  1:2159990/1‑56 (MQ=255)
                                                                   gAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGc                >  1:2321399/1‑56 (MQ=255)
                                                                    |                                                                    
CTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTG  >  minE/1260247‑1260383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: