| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,260,327 | G→A | 7.5% | S199S (TCC→TCT) ‡ | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,260,327 | 0 | G | A | 7.5% | 157.2 / 3.1 | 53 | S199S (TCC→TCT) ‡ | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base G (29/20); new base A (2/2); total (31/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTG > minE/1260258‑1260397 | ttttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCag < 1:619757/70‑1 (MQ=255) tttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg < 1:3096416/70‑1 (MQ=255) tttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCag > 1:437424/1‑69 (MQ=255) ttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggag > 1:187155/1‑71 (MQ=255) aaGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggagg < 1:2070520/70‑1 (MQ=255) ggTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTct > 1:131527/1‑71 (MQ=255) gTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTc < 1:2303606/69‑1 (MQ=255) cAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGtt > 1:3410339/1‑71 (MQ=255) cAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGt < 1:3639974/70‑1 (MQ=255) aGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGtt > 1:3742534/1‑70 (MQ=255) gCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa < 1:2796313/71‑1 (MQ=255) cGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa > 1:1677033/1‑70 (MQ=255) cGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCa < 1:1812133/70‑1 (MQ=255) gTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg < 1:1619114/53‑1 (MQ=255) ttCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgctg > 1:990780/1‑62 (MQ=255) tCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACAcc > 1:1263240/1‑71 (MQ=255) cATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACTCct > 1:129980/1‑71 (MQ=255) ttCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg < 1:461562/48‑1 (MQ=255) ttCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCagg < 1:895122/48‑1 (MQ=255) tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa > 1:2105493/1‑71 (MQ=255) tCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa > 1:1426793/1‑71 (MQ=255) tCCCCACAGCACCACAACCTGCCTGTTTTCCAGAATCACCGGCCAGGAGGTCTGCTGTTCATACACCTCTa > 1:2720775/1‑71 (MQ=255) ccccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa < 1:1941037/70‑1 (MQ=255) ccccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa < 1:3739683/70‑1 (MQ=255) ccccACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTa < 1:871553/70‑1 (MQ=255) tccACAGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaa < 1:3656911/62‑3 (MQ=255) tccACAGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACttc < 1:3639044/66‑1 (MQ=255) ccACAGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATaaactt > 1:388237/1‑63 (MQ=255) cacaGCACCACGACATCGCTATGTTCCAGCACCAGCGGCCAACTGGTCTGCTGTTGATAAACttcac > 1:472205/1‑65 (MQ=255) cacaGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGt > 1:2882729/1‑54 (MQ=255) cacaGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg > 1:2913386/1‑70 (MQ=255) cacaGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAg > 1:223710/1‑70 (MQ=255) cacaACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgc > 1:945875/1‑43 (MQ=255) caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTctgc > 1:1539500/1‑41 (MQ=255) caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATa > 1:1245523/1‑49 (MQ=255) caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATa > 1:2285047/1‑49 (MQ=255) caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca > 1:2309483/1‑71 (MQ=255) caACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACcaca > 1:2305958/1‑71 (MQ=255) aaCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACAt < 1:3347492/71‑1 (MQ=255) aCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATg < 1:1341823/71‑1 (MQ=255) gCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACca < 1:1348772/62‑1 (MQ=255) gCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCgg < 1:714210/69‑1 (MQ=255) cTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCAt < 1:962572/71‑1 (MQ=255) gTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGa > 1:1690957/1‑71 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGa > 1:1892699/1‑55 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg < 1:818390/69‑1 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg < 1:2942994/69‑1 (MQ=255) ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACAGACCACATGCGGCATGATCa > 1:3198086/1‑71 (MQ=255) aGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtg < 1:866807/71‑1 (MQ=255) gAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGc > 1:2159990/1‑56 (MQ=255) gAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGc > 1:2321399/1‑56 (MQ=255) accAGCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGc > 1:2851313/3‑57 (MQ=255) tCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGtgct < 1:1325954/71‑1 (MQ=255) tCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGtgct < 1:3634788/71‑1 (MQ=255) ccAGCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCtgc > 1:387693/2‑67 (MQ=255) aGCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCag < 1:2109162/56‑2 (MQ=255) gCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCTGcgcagca > 1:1169819/1‑70 (MQ=255) gCGGCCAGGAGGTCTTCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATc < 1:663320/59‑1 (MQ=255) gCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGtgct < 1:771933/68‑1 (MQ=255) ggCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGcccc > 1:3421707/1‑71 (MQ=255) cAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACca > 1:375485/1‑40 (MQ=255) aggaggTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCtgc < 1:2057834/59‑2 (MQ=255) aggaggTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCtgc < 1:3389787/59‑2 (MQ=255) ggaggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTg < 1:3830754/71‑1 (MQ=255) | TTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTG > minE/1260258‑1260397 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |