| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,260,374 | T→G | 13.0% | M184L (ATG→CTG) | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,260,374 | 0 | T | G | 13.0% | 127.6 / 16.0 | 61 | M184L (ATG→CTG) | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base T (22/31); new base G (5/3); total (27/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGTTCATA > minE/1260304‑1260441 | cTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCAt < 1:962572/71‑1 (MQ=255) gTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGa > 1:1690957/1‑71 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg < 1:2942994/69‑1 (MQ=255) ttttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATg < 1:818390/69‑1 (MQ=255) ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACAGACCACATGCGGCATGATCa > 1:3198086/1‑71 (MQ=255) aGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtg < 1:866807/71‑1 (MQ=255) tCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGtgct < 1:3634788/71‑1 (MQ=255) tCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGtgct < 1:1325954/71‑1 (MQ=255) ccAGCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCtgc > 1:387693/2‑67 (MQ=255) gCGGCCAGGAGGTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCTGcgcagca > 1:1169819/1‑70 (MQ=255) gCGGCCAGGAGGTCTTCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATc < 1:663320/59‑1 (MQ=255) gCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGtgct < 1:771933/68‑1 (MQ=255) ggCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGcccc > 1:3421707/1‑71 (MQ=255) aggaggTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCtgc < 1:3389787/59‑2 (MQ=255) aggaggTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCtgc < 1:2057834/59‑2 (MQ=255) ggaggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTg < 1:3830754/71‑1 (MQ=255) aggTTTGCTGTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCTgcgc > 1:361874/1‑58 (MQ=255) aggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaa < 1:1793571/71‑1 (MQ=255) ggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGcccc > 1:751161/1‑63 (MQ=255) gTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGtt > 1:3712378/1‑66 (MQ=255) tCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCTCCGGTTGaataa < 1:412997/71‑1 (MQ=255) tCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaataa < 1:271147/71‑1 (MQ=255) tCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaataa < 1:57562/71‑1 (MQ=255) ttgctgTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCTGCGCAGCACCGGTAGagtaat > 1:1378925/2‑71 (MQ=255) ctgctgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgtg > 1:1325961/1‑53 (MQ=255) tgctgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATc > 1:2944443/1‑71 (MQ=255) gctgTTCATACACTTCCATCGAACCGACTACGCGCGGCAGGATCACCTGCGCAGCACCGGTAGAGTAATct > 1:1155832/1‑70 (MQ=255) gctgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCgg < 1:1380532/60‑1 (MQ=255) gctgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGt > 1:630130/1‑61 (MQ=255) gctgTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGt > 1:1372114/1‑61 (MQ=255) gTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATcgccg > 1:1053171/1‑71 (MQ=255) ttCATACACCTCTACAGAACCGACCACATGCGGCATGATc < 1:566735/40‑1 (MQ=255) ttCATACACCTCTACAGAACCGACCACATGCGGCATGATc < 1:2192306/40‑1 (MQ=255) ttCATACACCTCTACAGAACCGACCACATGCGGCATGATc < 1:475635/40‑1 (MQ=255) ttCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgt < 1:754509/46‑1 (MQ=255) ttCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATcgccgc < 1:1111099/71‑1 (MQ=255) ttCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATcgccgc < 1:2307469/71‑1 (MQ=255) tCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCtgt > 1:1041316/1‑45 (MQ=255) cATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCt < 1:1212606/70‑1 (MQ=255) aTACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTAt < 1:3655790/71‑1 (MQ=255) cacCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGc < 1:3529131/70‑1 (MQ=255) acCTCTACAGAACCGACCACATGCGGCATGATCACCtgtg > 1:1875613/1‑40 (MQ=255) ccTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCg < 1:2827848/71‑1 (MQ=255) ctctACAGAACCGACCACATGCGGCATGATCACCtgtg > 1:1702079/1‑38 (MQ=255) ctctACAGAACCGACCACATGCGGCATGATCACCtgtg > 1:192281/1‑38 (MQ=255) tctACAGAACCGACCACATGCGGCAGGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTa < 1:3119089/63‑1 (MQ=255) tACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGaata < 1:1546323/51‑1 (MQ=255) tACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGaa > 1:1202165/1‑70 (MQ=255) tACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGaa > 1:2084887/1‑70 (MQ=255) cAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAAt < 1:1862933/69‑1 (MQ=255) gAACCGACCACATGCGGCATGATCACCTGTGCTGccc > 1:3703121/1‑37 (MQ=255) aCCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAAccgc < 1:1433177/71‑1 (MQ=255) gACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAAt < 1:975044/62‑1 (MQ=255) gACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAAccgcccg > 1:3915654/1‑71 (MQ=255) gACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAAc < 1:1035796/65‑1 (MQ=255) ccACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAAccgcccgc > 1:3479119/1‑70 (MQ=255) cacaTGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAAccgcccgc > 1:1028066/1‑69 (MQ=255) caTGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAg < 1:2647557/70‑1 (MQ=255) aTGCGGCATGATCACCTGTGCTGCCCCGGTTGaataat < 1:713586/38‑1 (MQ=255) gCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACC‑CCCGCCAGGTTCa > 1:1644282/1‑71 (MQ=255) gCGGCATGATCACCTGTGCTGCCACGGTTGaataat > 1:1855246/1‑36 (MQ=255) gCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGTTCata < 1:2762918/71‑1 (MQ=255) tGATCACCTGTGCTGCCCCGGTTGAATAATcgccgc < 1:681777/36‑1 (MQ=255) | CTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGTTCATA > minE/1260304‑1260441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |