| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,268,310 | T→A | 14.9% | I99F (ATC→TTC) | pgsA ← | phosphatidylglycerophosphate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,268,310 | 0 | T | A | 14.9% | 112.9 / 12.6 | 47 | I99F (ATC→TTC) | pgsA | phosphatidylglycerophosphate synthetase |
| Reads supporting (aligned to +/- strand): ref base T (15/25); new base A (2/5); total (17/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
CACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGA > minE/1268242‑1268375 | cACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGatca > 1:3503007/1‑71 (MQ=255) aCGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGatcat > 1:280320/1‑71 (MQ=255) aCGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCAAGGGCGatcat > 1:3182565/1‑71 (MQ=255) cGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGataga > 1:853181/1‑67 (MQ=255) gCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCg < 1:3197070/71‑1 (MQ=255) gCTACTGCGTTTACCCAACTCCCCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCg < 1:601460/71‑1 (MQ=255) tACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGtt > 1:1932873/1‑71 (MQ=255) cTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGatc > 1:1245592/1‑63 (MQ=255) cTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGtt < 1:3380479/69‑1 (MQ=255) cTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTgc > 1:1612862/1‑71 (MQ=255) tGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGatca < 1:2682499/63‑1 (MQ=255) cGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTgccgc < 1:2284828/71‑1 (MQ=255) cGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTgccg < 1:317330/70‑1 (MQ=255) tttACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCg < 1:2962703/62‑1 (MQ=255) tttACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTgcc > 1:1943686/1‑67 (MQ=255) tttACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGccgccg > 1:1725269/1‑71 (MQ=255) tttACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGAACATCGtt < 1:3015597/64‑1 (MQ=255) ttACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCgg > 1:2419404/1‑71 (MQ=255) tACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCgg > 1:1211962/1‑70 (MQ=255) aCCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGt < 1:3127376/70‑1 (MQ=255) aCCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGt < 1:1755971/70‑1 (MQ=255) cccAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGTa < 1:1993213/70‑1 (MQ=255) cAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGTCGATCATCGTTGccgccg < 1:526647/65‑1 (MQ=255) cAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGccgccg < 1:531381/65‑1 (MQ=255) aaCTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGtt > 1:2352412/1‑57 (MQ=255) aCTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGTAATGTc < 1:2333344/71‑1 (MQ=255) aCTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGAACATCGTTGCCGCCGGt < 1:74767/65‑1 (MQ=255) cTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGTAATGTca < 1:3660674/71‑1 (MQ=255) ccGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGaacatc > 1:1729028/1‑50 (MQ=255) cGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGTAATGTcacc > 1:3240817/1‑70 (MQ=255) ccatccatTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGTAATGTcacccac > 1:3792106/1‑71 (MQ=255) catccatTCGCGTAGCGCAGAAATAATAATTTCACTGGCGATCATCg < 1:3577692/47‑1 (MQ=255) catTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGc < 1:487229/70‑1 (MQ=255) catTCGCGTAGCGCAGAAATAATAATTTCACGGGCGAACATCGTTGCCGCCGGTAATGTCACCCACCAGCt < 1:1437829/71‑1 (MQ=255) ttCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGt < 1:279861/42‑1 (MQ=255) gcgTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGa < 1:412158/70‑1 (MQ=255) cgTAGCGCAGAAATAATAATTTCACTGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATa < 1:873458/71‑1 (MQ=255) gTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATaa > 1:449736/1‑71 (MQ=255) aGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCtg > 1:2368594/1‑63 (MQ=255) cgcAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCt < 1:2810833/71‑1 (MQ=255) cgcAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCt < 1:1888999/71‑1 (MQ=255) aaaTAATAATTTCACGGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGtt < 1:1338519/71‑1 (MQ=255) ataataatTTCACGGGCGATCATCGTTGCCGCCGGTAAt > 1:3727602/1‑39 (MQ=255) aatTTCACGGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCt < 1:3659134/48‑1 (MQ=255) aatTTCACGGGCGAACATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCg > 1:1056175/1‑61 (MQ=255) atTTCACGGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCtgt < 1:1968193/49‑1 (MQ=255) tttCACGGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCAc > 1:1832784/1‑70 (MQ=255) tCACGGGCGAACATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCa < 1:1349/70‑1 (MQ=255) cACGGGCGAACATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCa < 1:3829173/69‑1 (MQ=255) aCGGGCGATCATCGTTGCCGCCGGTAATGTcacccacc < 1:3298170/38‑1 (MQ=255) cGGGCGAACATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGc > 1:689738/1‑71 (MQ=255) gggCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGtt < 1:3816935/56‑1 (MQ=255) gCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTAc > 1:3345442/1‑56 (MQ=255) cGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGa > 1:3945438/1‑69 (MQ=255) atcatcGTTGCCGCCGGTAATGTCACCCACCAGCTGTGAt < 1:2941984/40‑1 (MQ=255) | CACGCTACTGCGTTTACCCAACTCCGCCATCCATTCGCGTAGCGCAGAAATAATAATTTCACGGGCGATCATCGTTGCCGCCGGTAATGTCACCCACCAGCTGTGATAATGCTCGGTTACCAGCACCATGGCGA > minE/1268242‑1268375 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |