| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,299,487 | G→A | 30.8% | intergenic (‑412/+78) | cobU ← / ← yeeX | bifunctional cobinamide kinase and cobinamide phosphate guanylyltransferase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,299,487 | 0 | G | A | 30.8% | 48.2 / 28.3 | 39 | intergenic (‑412/+78) | cobU/yeeX | bifunctional cobinamide kinase and cobinamide phosphate guanylyltransferase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (20/7); new base A (5/7); total (25/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.49e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCA > minE/1299418‑1299548 | cTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCggatgagga < 1:3250968/71‑1 (MQ=255) tAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGgatgat < 1:1762734/71‑1 (MQ=255) gTAATAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACgg < 1:2348389/67‑1 (MQ=255) aTAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATaca > 1:3738676/1‑71 (MQ=255) aTAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATaca > 1:92975/1‑71 (MQ=255) tAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATaca > 1:3796255/1‑70 (MQ=255) aGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATaca > 1:2246264/1‑69 (MQ=255) aaTTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCggatgaggatg < 1:1116850/53‑1 (MQ=255) aTCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCggatgaggatg < 1:3350800/49‑1 (MQ=255) aTCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCggatgaggatg < 1:2820589/49‑1 (MQ=255) aTCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGAt > 1:3014685/1‑68 (MQ=255) gAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATca < 1:2027807/71‑1 (MQ=255) aTGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaat > 1:604844/1‑71 (MQ=255) aTGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaat > 1:600241/1‑71 (MQ=255) tggtggCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaat > 1:910351/1‑70 (MQ=255) ggtggCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaat > 1:2527057/1‑69 (MQ=255) gtggCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatgag > 1:1744817/1‑71 (MQ=255) gCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatgagca > 1:1586721/1‑70 (MQ=255) gCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatgaaca > 1:1886354/1‑70 (MQ=255) gACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACg > 1:3731390/1‑40 (MQ=255) gACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGAt > 1:3283717/1‑45 (MQ=255) aCTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGt < 1:593159/41‑1 (MQ=255) cTATAATTTCGATTGGTTCTCGGATGAGGATTATGACGGTGATACACAGATAAGAATcaatgagcaatgag < 1:3739297/71‑1 (MQ=255) ttaATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAgg < 1:2018437/69‑1 (MQ=255) taATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaat > 1:2751144/1‑58 (MQ=255) taATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaat > 1:2055319/1‑58 (MQ=255) aaTTTCTATTGGTTCTCGGATGAGGATGATGACGGt > 1:504936/1‑36 (MQ=255) aaTTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGtt > 1:2856136/1‑71 (MQ=255) aaTTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGtt > 1:3332354/1‑71 (MQ=255) aaTTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGt < 1:3812496/70‑1 (MQ=255) aTTTCGATTGGTTCTCGGATGAGAATGATGACTGTGATACACAGATAAGAATCAATGAGCAATGAGGGtt < 1:1358751/70‑1 (MQ=255) aTTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATcaatgagcaatga < 1:3431210/65‑1 (MQ=255) tttCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGc < 1:2733198/71‑1 (MQ=255) tCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGcc < 1:3546122/70‑1 (MQ=255) tCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGcc < 1:1949429/70‑1 (MQ=255) tCGATTGGTTCTCGGATGAGAATGATGACCGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGcc < 1:122121/70‑1 (MQ=255) cGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTg > 1:98555/1‑67 (MQ=255) cGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCgg > 1:799681/1‑71 (MQ=255) cGATTGGTTCTCGGATGAGAATGATGACGGTGATa > 1:1583546/1‑35 (MQ=255) gATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatgagcaatga < 1:3513277/60‑1 (MQ=255) gATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatgagcaatga < 1:3089664/60‑1 (MQ=255) gATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCggg > 1:1976288/1‑71 (MQ=255) tGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatgagc > 1:1376066/1‑52 (MQ=255) tctcGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCt > 1:1686008/1‑71 (MQ=255) ggatgaggatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCa < 1:826620/69‑1 (MQ=255) ggatgagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTc < 1:1155072/68‑1 (MQ=255) gatgagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCa > 1:2661686/1‑68 (MQ=255) gaggatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGtt > 1:3904441/1‑50 (MQ=255) gatgatGACGGTGATACACAGATAAGAATcaatgagcaatgag < 1:3556131/43‑1 (MQ=255) | CTTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCA > minE/1299418‑1299548 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |