| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,309,899 | G→A | 53.3% | R31R (CGC→CGT) | yefM ← | antitoxin of the YoeB‑YefM toxin‑antitoxin system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,309,899 | 0 | G | A | 53.3% | 5.2 / 79.2 | 60 | R31R (CGC→CGT) | yefM | antitoxin of the YoeB‑YefM toxin‑antitoxin system |
| Reads supporting (aligned to +/- strand): ref base G (15/13); new base A (12/20); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTT > minE/1309829‑1309969 | gTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTgg > 1:2392121/1‑71 (MQ=255)gTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTgg < 1:2303759/71‑1 (MQ=255) aGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGc > 1:172098/1‑70 (MQ=255) gATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGc > 1:1392467/1‑69 (MQ=255) gATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGc > 1:3142405/1‑69 (MQ=255) gCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGtaat < 1:1938994/71‑1 (MQ=255) gCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaa > 1:3373805/1‑70 (MQ=255) tctcTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGa < 1:616177/62‑1 (MQ=255) ccAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCggg > 1:2630721/1‑71 (MQ=255) aGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGc < 1:2671103/71‑1 (MQ=255) aGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGc < 1:3198811/71‑1 (MQ=255) cGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGAt < 1:3375388/63‑1 (MQ=255) gAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTACATTCTGGCGAGTAATAAGGATCGGGGCATg < 1:3128761/71‑1 (MQ=255) aGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGa < 1:692245/71‑1 (MQ=255) gTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaata < 1:3220600/55‑1 (MQ=255) gTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaata < 1:3215993/55‑1 (MQ=255) gTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGa < 1:3035727/70‑1 (MQ=255) gTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGAt < 1:1275924/71‑1 (MQ=255) tGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGAt > 1:1465473/1‑69 (MQ=255) tGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCt > 1:2163195/1‑71 (MQ=255) tATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTc < 1:3616012/71‑1 (MQ=255) aTTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAg > 1:1547878/1‑52 (MQ=255) aTTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:1915203/1‑70 (MQ=255) aTTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:329914/1‑70 (MQ=255) aTTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:829077/1‑70 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCaa < 1:2221060/71‑1 (MQ=255) tcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAAc > 1:3280305/1‑71 (MQ=255) cttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCAt > 1:761665/1‑60 (MQ=255) cttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCAt > 1:2552350/1‑60 (MQ=255) cGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGc < 1:2537929/70‑1 (MQ=255) gAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCtt < 1:1225046/71‑1 (MQ=255) aGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCttt < 1:3131965/71‑1 (MQ=255) gTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGAt < 1:334803/46‑1 (MQ=255) gTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGAt < 1:1676780/46‑1 (MQ=255) tGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtc > 1:949143/1‑70 (MQ=255) aCATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcat > 1:3335116/1‑70 (MQ=255) cATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca < 1:137649/71‑1 (MQ=255) aTCAGATCACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCAt < 1:156310/49‑1 (MQ=255) tCAGAACACTAGCCTCTCCATTCTGACGTGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatc < 1:3027107/68‑1 (MQ=255) tCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca < 1:239735/69‑1 (MQ=255) tCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTcatcat < 1:3259485/70‑1 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttg > 1:2770689/1‑71 (MQ=255) aGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttg < 1:672221/70‑1 (MQ=255) aaCACAAGCCTCTCCATTCTGGCGAGTAATAAGGAt > 1:1385683/1‑36 (MQ=255) aaCACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGc < 1:1394240/42‑1 (MQ=255) aaCACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatc < 1:1317114/64‑1 (MQ=255) acacAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGc < 1:2528690/41‑1 (MQ=255) acacAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGc > 1:1921347/1‑71 (MQ=255) cacaAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCAt > 1:2849870/1‑42 (MQ=255) cacaAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGcc < 1:203904/71‑1 (MQ=255) caAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGa > 1:226798/1‑71 (MQ=255) aaGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgttg < 1:1669237/66‑1 (MQ=255) gCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca > 1:109406/1‑58 (MQ=255) gCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACa > 1:803732/1‑70 (MQ=255) tctcCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGcc < 1:3465832/63‑1 (MQ=255) tcCATTCTGGCGAGTAATAAGGATCGGGGCATGAta > 1:1616365/1‑35 (MQ=255) tcCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAAtt < 1:2021750/69‑1 (MQ=255) cATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGa > 1:2611727/1‑71 (MQ=255) aTTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGAc > 1:1789573/1‑71 (MQ=255) aTTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgttg < 1:3687286/56‑1 (MQ=255) ttCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAAc < 1:3786606/38‑1 (MQ=255) tCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGa > 1:858404/1‑58 (MQ=255) gCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCtt < 1:693974/71‑1 (MQ=255) | GTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTT > minE/1309829‑1309969 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |