| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,314,255 | T→C | 56.6% | H170H (CAT→CAC) | hisB → | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,314,255 | 0 | T | C | 56.6% | 49.2 / 127.4 | 106 | H170H (CAT→CAC) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (19/27); new base C (28/32); total (47/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 106 total aligned reads displayed.
CTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAA > minE/1314186‑1314323 | cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATg < 1:805249/71‑1 (MQ=255) cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGt < 1:3241858/69‑1 (MQ=255) cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGt < 1:2880957/69‑1 (MQ=255) cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTa < 1:2988896/70‑1 (MQ=255) cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAg > 1:1221556/1‑71 (MQ=255) aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTg < 1:2765797/71‑1 (MQ=255) aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTg > 1:629255/1‑71 (MQ=255) ccGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGc > 1:1629869/1‑71 (MQ=255) cgcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGc < 1:790683/70‑1 (MQ=255) cgcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCg > 1:2123979/1‑71 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCg > 1:2668384/1‑70 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGt < 1:1285317/71‑1 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:2706131/71‑1 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:1811346/71‑1 (MQ=255) gAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAg < 1:2923321/64‑1 (MQ=255) gAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa < 1:1837998/71‑1 (MQ=255) cccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATAc > 1:3615924/1‑70 (MQ=255) ccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACTTGACCGTTACGCTCATGTAGTGCGt < 1:2774198/64‑1 (MQ=255) cTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGt > 1:3425567/1‑59 (MQ=255) cTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCa > 1:3176641/1‑70 (MQ=255) tGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCa > 1:902987/1‑69 (MQ=255) gAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCa > 1:1849963/1‑68 (MQ=255) aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCaaa < 1:1062828/69‑1 (MQ=255) aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa < 1:1683564/69‑1 (MQ=255) aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa < 1:3025317/69‑1 (MQ=255) cTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAgaga < 1:729864/71‑1 (MQ=255) ggCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg > 1:1776207/1‑71 (MQ=255) aaTGATTGGCGCGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg > 1:2151753/1‑67 (MQ=255) aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg > 1:1044072/1‑67 (MQ=255) aTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGc > 1:3588101/1‑67 (MQ=255) aTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAt > 1:172241/1‑71 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1938453/1‑71 (MQ=255) agATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3122501/2‑71 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAg > 1:2739333/1‑44 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCg > 1:2473941/1‑48 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:3540700/41‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt < 1:1012857/69‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:879541/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:3540493/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:1381407/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:2839939/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa < 1:1402223/71‑1 (MQ=255) ttGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGAc > 1:3244573/1‑71 (MQ=255) gCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt < 1:2342047/70‑1 (MQ=255) gAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACTCAGa < 1:3478605/61‑1 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:312195/71‑1 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAg < 1:523066/70‑1 (MQ=255) aaCTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgtg < 1:2545962/71‑1 (MQ=255) aaCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgtg < 1:715637/71‑1 (MQ=255) aaCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgtg < 1:877530/71‑1 (MQ=255) aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGa < 1:1025287/56‑1 (MQ=255) cTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg > 1:859462/1‑71 (MQ=255) tCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTgg > 1:762788/1‑71 (MQ=255) tCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg > 1:1688227/1‑70 (MQ=255) tCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTgg > 1:741924/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTgg > 1:888556/1‑70 (MQ=255) cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:2059847/1‑71 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt < 1:3079058/54‑1 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc < 1:3384690/70‑1 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc < 1:1037950/70‑1 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCt < 1:473869/71‑1 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCt > 1:2164684/1‑71 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCt < 1:1326882/71‑1 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCt > 1:141554/1‑71 (MQ=255) aCCAGACGTGACCGTTACGCTCACGTAGTGCGTAATAcc < 1:145208/39‑1 (MQ=255) aCCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTgg < 1:1266480/69‑1 (MQ=255) ccAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTg < 1:453307/71‑1 (MQ=255) ccAGACGTGACCGTTACGCTCATGTAGTGCGTAATAACAAAGAGACGCAGATTGACGTTCAGGTGTGGCTg > 1:3388199/1‑71 (MQ=255) cAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAagat > 1:931663/1‑42 (MQ=255) cAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTg > 1:1305351/1‑70 (MQ=255) cAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTg > 1:1116108/1‑70 (MQ=255) cAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTgg < 1:3750350/71‑1 (MQ=255) cAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTgg < 1:2841107/71‑1 (MQ=255) aGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGa > 1:3673252/1‑71 (MQ=255) aGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGa < 1:130790/71‑1 (MQ=255) gACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGAt < 1:3632440/71‑1 (MQ=255) gACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGAt > 1:1189277/1‑71 (MQ=255) aCGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAgaga > 1:2516031/1‑41 (MQ=255) gTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTTTGGCTGGATCGt > 1:2775008/1‑71 (MQ=255) gTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGt > 1:3437078/1‑71 (MQ=255) tGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTg < 1:2848290/71‑1 (MQ=255) aCCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGa < 1:1095086/70‑1 (MQ=255) aCCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGa < 1:2186376/70‑1 (MQ=255) ccGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAg > 1:1091005/1‑71 (MQ=255) ccGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAg > 1:3062413/1‑71 (MQ=255) cGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAg > 1:2224746/1‑70 (MQ=255) gTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGt < 1:3573643/71‑1 (MQ=255) ttACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAgg > 1:1136128/1‑69 (MQ=255) ttACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTg > 1:3266711/1‑71 (MQ=255) aCGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTgg < 1:3307130/70‑1 (MQ=255) aCGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTgg < 1:1789041/70‑1 (MQ=255) aCGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGTTgg < 1:1719278/70‑1 (MQ=255) aCGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTgg < 1:1753802/70‑1 (MQ=255) gCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTgg < 1:1210082/68‑1 (MQ=255) gCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcag > 1:158610/1‑71 (MQ=255) tCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGtt < 1:356440/40‑1 (MQ=255) tCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcagc > 1:3179226/1‑70 (MQ=255) tCACGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:2330067/35‑1 (MQ=255) tCACGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:2059214/35‑1 (MQ=255) aTGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCaa < 1:3337898/70‑1 (MQ=255) | CTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAA > minE/1314186‑1314323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |