| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 228,538 | A→G | 8.2% | S55S (AGT→AGC) | phoE ← | outer membrane phosphoporin protein E |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 228,538 | 0 | A | G | 8.2% | 231.7 / 10.0 | 85 | S55S (AGT→AGC) | phoE | outer membrane phosphoporin protein E |
| Reads supporting (aligned to +/- strand): ref base A (49/29); new base G (4/3); total (53/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01 | |||||||||||
CTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGCCATAGACATCC > minE/228468‑228607 | cTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATaa > 1:3150205/1‑71 (MQ=255) ttCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAAc > 1:425616/1‑71 (MQ=255) ttCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAAc > 1:3209535/1‑71 (MQ=255) cccAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAActc > 1:3380070/1‑71 (MQ=255) cccAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAAc < 1:551892/69‑1 (MQ=255) aaCGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTgg < 1:314234/71‑1 (MQ=255) gACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGt > 1:1422945/1‑69 (MQ=255) gACCGTAGCCAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCg > 1:157693/1‑71 (MQ=255) gACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTc > 1:3404868/1‑70 (MQ=255) gACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTc > 1:2812419/1‑70 (MQ=255) gACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCg > 1:844957/1‑71 (MQ=255) aCCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGc < 1:3226807/71‑1 (MQ=255) aTAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGACACCAAAACGGATATAACTCTGGTCGCCAt < 1:492455/71‑1 (MQ=255) aTAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCAt < 1:1180725/71‑1 (MQ=255) aTAACCAGTCAGTTGATCGTTAATTTGTGTTTCGACTTTGAAACCAAAACGGATATAACTCTGGTCGCCAt > 1:1137391/1‑71 (MQ=255) tagccAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc < 1:2273121/68‑1 (MQ=12) tagccAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc > 1:1835283/4‑71 (MQ=12) tagccAGTGAGTTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATc > 1:289367/4‑71 (MQ=12) aCCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATaa > 1:722913/1‑55 (MQ=255) aCCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCtt < 1:963391/71‑1 (MQ=255) aCCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCtt < 1:2628137/71‑1 (MQ=255) ccAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCttt < 1:166367/71‑1 (MQ=255) cagtcagtTGATCGTTAATTTGTGTTTCGCGTTTGAAACCAAAACGGATATAACTCTGGTc > 1:700112/1‑61 (MQ=255) agtgagtTGATCGTTAATTTGGGTTTCGCCTTTAAAACCCAGACGCGCATAGCTCTGGTCGCCATCtttcg < 1:3266790/71‑3 (MQ=19) gtgagtTGATCGTTAATTTGGGTTTCGCCTTTAAAAGCCAGACGCGCATAGCTCTGGTCGCCATCt < 1:3865825/63‑1 (MQ=255) ttGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTc > 1:3925720/1‑54 (MQ=255) ttGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGt > 1:292341/1‑71 (MQ=255) ttGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGt > 1:3127936/1‑71 (MQ=255) aTCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCtt < 1:300585/59‑1 (MQ=255) tCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTAt > 1:3878702/1‑70 (MQ=255) tCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATc < 1:3818685/71‑1 (MQ=255) tCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATc < 1:2265918/71‑1 (MQ=255) cGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATc > 1:155143/1‑70 (MQ=255) gTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCa > 1:1034926/1‑70 (MQ=255) gTTAATTTGTGTTTCGCCTTTGAAAACAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCa < 1:2401921/70‑1 (MQ=255) ttAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCa > 1:694900/1‑51 (MQ=255) ttAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCa > 1:2862300/1‑51 (MQ=255) ttAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACt < 1:1135909/71‑1 (MQ=255) ttAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACt > 1:43192/1‑71 (MQ=255) tAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACt > 1:1290886/1‑70 (MQ=255) aaTTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTc < 1:1224362/70‑1 (MQ=255) tttGTGTTTCGCCTTTGAAACCAAAACGGATATAATTCTGTTCg > 1:3542332/1‑44 (MQ=255) tttGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTAc < 1:1970715/54‑1 (MQ=255) tttGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTAc < 1:1862248/54‑1 (MQ=255) ttGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCata > 1:1125748/1‑70 (MQ=255) ttGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCata > 1:787080/1‑70 (MQ=255) gtgtTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGc > 1:187798/1‑55 (MQ=255) tgtTTCGCCTTTGAAACCAAAACGGATATAACTCTg < 1:2717931/36‑1 (MQ=255) tgtTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCAc > 1:1557438/1‑62 (MQ=255) tttCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATg < 1:1654923/70‑1 (MQ=255) tCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATc > 1:1778927/1‑41 (MQ=255) tCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATc > 1:753096/1‑41 (MQ=255) tCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTa > 1:1008030/1‑45 (MQ=255) tCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCa < 1:1959772/70‑1 (MQ=255) tCGCATTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTc > 1:2035740/1‑60 (MQ=255) cGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTAc > 1:3409579/1‑45 (MQ=255) cGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATg > 1:372582/1‑71 (MQ=255) cGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATATTTACTGGCGTTATCACTCATATAATGCATg > 1:3378649/1‑71 (MQ=255) cTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTAACTGGCGt > 1:60679/1‑48 (MQ=255) tttGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTa < 1:3373877/49‑1 (MQ=255) ttGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTAt > 1:3632668/1‑49 (MQ=255) ttGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTAt > 1:1628621/1‑49 (MQ=255) ttGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCtt > 1:2704674/1‑70 (MQ=255) ttGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCtt > 1:3665960/1‑70 (MQ=255) tGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCttt > 1:3320600/1‑70 (MQ=255) tGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCttt > 1:1626692/1‑70 (MQ=255) tGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCtt < 1:2217319/69‑1 (MQ=255) gAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTa < 1:2628581/70‑1 (MQ=255) aaaCCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAAc > 1:993791/1‑71 (MQ=255) aaaCCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAAc < 1:3341268/71‑1 (MQ=255) aaCCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCata < 1:3870080/53‑1 (MQ=255) aaCCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACt < 1:1959813/71‑1 (MQ=255) aaCCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACt < 1:3910593/71‑1 (MQ=255) aCCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACtt > 1:2276480/1‑71 (MQ=255) cAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTc > 1:2790916/1‑47 (MQ=255) cAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTg > 1:1979329/1‑71 (MQ=255) aaCGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCAtata > 1:549648/1‑49 (MQ=255) aaCGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGcc > 1:1347099/1‑70 (MQ=255) aaCGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGc > 1:1118761/1‑69 (MQ=255) aCGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGCCa > 1:2272089/1‑70 (MQ=255) ggATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGCCa > 1:90862/1‑68 (MQ=255) gATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGCCATAGa < 1:1826869/71‑1 (MQ=255) tataACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGCCATAGa < 1:1811353/69‑1 (MQ=255) tataACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGCCATAGACa < 1:3520924/71‑1 (MQ=255) ataACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCttt > 1:2493854/1‑54 (MQ=255) ataACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCttt > 1:2824907/1‑54 (MQ=255) ataACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCttt > 1:3611570/1‑54 (MQ=255) aaCTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGCCATAGACATc > 1:1997662/1‑70 (MQ=255) aCTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGCCATAGACATcc < 1:2778880/70‑1 (MQ=255) aCTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGCCATAGACATc > 1:2298119/1‑69 (MQ=255) aCTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGCCATAGACATc > 1:2916033/1‑69 (MQ=255) aCTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGCCATAGACATc > 1:3676129/1‑69 (MQ=255) aCTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGCCATAGACATc > 1:1612215/1‑69 (MQ=255) aCTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAAAtt > 1:1309816/1‑56 (MQ=255) | CTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAACCAAAACGGATATAACTCTGGTCGCCATCTTTACTGGCGTTATCACTCATATAATGCATGGCTTTAACTTTGCCATAGACATCC > minE/228468‑228607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |