| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,337,480 | A→G | 43.3% | S5S (AGT→AGC) | wcaF ← | predicted acyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,337,480 | 0 | A | G | 43.3% | 20.8 / 65.4 | 57 | S5S (AGT→AGC) | wcaF | predicted acyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (16/16); new base G (12/12); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTA > minE/1337418‑1337546 | gCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATc > 1:2205602/1‑71 (MQ=255) ccaTAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCACTTAAATCTTGCa > 1:1093028/1‑71 (MQ=255) aTAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCAtt > 1:3313574/1‑71 (MQ=255) ttGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCACTTAAATCTTGCAtt > 1:3074695/1‑67 (MQ=255) cACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCACTTAAAt < 1:2337958/56‑1 (MQ=255) aCTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCACTTAAAACTTGCATTGGTTTTcc < 1:2002535/71‑1 (MQ=255) tttAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCACTTAAATCtt > 1:89991/1‑56 (MQ=255) tAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCACTTAAATc < 1:778262/52‑1 (MQ=255) tAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCACTTAAATCTTGCATTGGTTTTCCTta > 1:1419153/1‑70 (MQ=255) aaTAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCACTTAAATCTTGCATTGGTTTTCCTtat < 1:754543/70‑1 (MQ=255) tAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTc > 1:2017027/1‑63 (MQ=255) tAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATAtt < 1:2862722/71‑1 (MQ=255) gCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATAtt > 1:2381423/1‑69 (MQ=255) gCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATAtt > 1:3503435/1‑69 (MQ=255) ccGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCa < 1:887072/70‑1 (MQ=255) gTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATAtt < 1:533451/67‑1 (MQ=255) gTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATAtt < 1:2617158/67‑1 (MQ=255) ttGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGtttt < 1:3513855/57‑1 (MQ=255) ttGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCACTTa < 1:1887573/40‑1 (MQ=255) ttGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCACTTAAATCTTGCATTGGttt > 1:1501310/1‑56 (MQ=255) tGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACt > 1:2737794/1‑71 (MQ=255) gccgccCCGGAACCCTTTCGGCACCGAGAAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACtt > 1:3255473/1‑71 (MQ=255) cgccCCGGAACCCTTTCGGCACCGAGAAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTg < 1:52316/71‑1 (MQ=255) gccCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGt < 1:227518/71‑1 (MQ=255) gccCCGGAACCCTTTCGGCACCGAGAAACCACTTAAATCtt < 1:2869195/41‑1 (MQ=255) cGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGc > 1:247352/1‑39 (MQ=255) cGGAACCCTTTCGGCACCGAGAAACCACTTAAATCTTGCATTGGTTTTCCTtata < 1:1398032/55‑1 (MQ=255) gAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGtt < 1:607589/66‑1 (MQ=255) gAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTtatat > 1:711741/1‑70 (MQ=255) aaCCCTTTCGGCACCGAGAAACC‑CTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAg > 1:453588/1‑71 (MQ=255) cTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACttt < 1:2801139/58‑1 (MQ=255) cTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCt > 1:3746158/1‑71 (MQ=255) tttCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCt < 1:96208/43‑1 (MQ=255) tttCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCtt > 1:3797425/1‑71 (MQ=255) tCGGCACCGAGAAACCACTTAAATCTTGCATTGGt < 1:1379527/35‑1 (MQ=255) tCGGCACCGAGAAACCACTTAAATCTTGCATTGGt < 1:3916651/35‑1 (MQ=255) cGGCACCGAGAAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTc < 1:1384997/70‑1 (MQ=255) cACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATAtt < 1:3735599/43‑1 (MQ=255) aCCGAGAAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCtt > 1:1469476/1‑71 (MQ=255) aCCGAGAAACCACTTAAATCTTGCATTGGTTTTCC‑TATATTCAGACTTTGTTATATAAGGCTTTCGTCtt > 1:3888751/1‑70 (MQ=255) ccGAGAAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACt < 1:1809460/47‑1 (MQ=255) cGAGAAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGt < 1:2581520/50‑1 (MQ=255) cGAGAAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCttt > 1:3933833/1‑70 (MQ=255) cGAGAAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCttt > 1:3483169/1‑70 (MQ=255) gagaAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTtata < 1:2249493/53‑1 (MQ=255) gagaAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGtt > 1:2928023/1‑50 (MQ=255) gagaAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCttt > 1:975364/1‑69 (MQ=255) gagaAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTATTATATAAGGCTTTCGTCttt > 1:1210526/1‑69 (MQ=255) agaAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAg < 1:3662616/71‑1 (MQ=255) agaAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAg > 1:1618926/1‑71 (MQ=255) gaAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAgg > 1:2623107/1‑71 (MQ=255) gaAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAgg > 1:1124306/1‑71 (MQ=255) gaAACCACTTAAATCTTGCATTTGTTTTCCTTATATTCAGACTTTGTTATATAAgg > 1:2603864/1‑56 (MQ=255) gaAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAgg > 1:1670342/1‑56 (MQ=255) aaaCCGCTTAAATCTTGCATTGGTTTTCCTTATAtt < 1:3623428/36‑1 (MQ=255) aaCCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGgta < 1:2440866/71‑1 (MQ=255) aCCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTtatat < 1:437787/49‑1 (MQ=255) | GCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCACTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTA > minE/1337418‑1337546 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |