| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,341,041 | C→T | 9.2% | A41T (GCG→ACG) | wcaB ← | predicted acyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,341,041 | 0 | C | T | 9.2% | 155.2 / 4.6 | 54 | A41T (GCG→ACG) | wcaB | predicted acyl transferase |
| Reads supporting (aligned to +/- strand): ref base C (32/17); new base T (2/3); total (34/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAAC > minE/1340972‑1341110 | cgGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGgccgcc < 1:1128082/71‑1 (MQ=255) aGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGTCca > 1:3000089/1‑69 (MQ=255) ggATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCca > 1:2810851/1‑64 (MQ=255) ggATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGAttg > 1:1635396/1‑71 (MQ=255) ggATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGAttg > 1:2863009/1‑71 (MQ=255) ggATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGAttg > 1:2959170/1‑71 (MQ=255) aTTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGAttgtt < 1:2845087/71‑1 (MQ=255) ttCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGccc > 1:1884652/1‑59 (MQ=255) ttCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGAttgtt > 1:338496/1‑69 (MQ=255) tCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAg > 1:17072/1‑71 (MQ=255) tCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAg > 1:1415351/1‑71 (MQ=255) tCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAg > 1:2367434/1‑71 (MQ=255) cATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCcaca < 1:1983058/60‑1 (MQ=255) cATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAg > 1:2791130/1‑70 (MQ=255) aTAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGa > 1:3950227/1‑71 (MQ=255) aaCCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCTGGGCCGCCCACAGATTGTTGAGGAc > 1:2285820/1‑70 (MQ=255) aaCCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACg > 1:931588/1‑71 (MQ=255) aCCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGTCCACAGATTGTTGAGGACGt > 1:795322/1‑71 (MQ=255) aCCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGa < 1:550278/68‑1 (MQ=255) aCCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGt > 1:3482903/1‑71 (MQ=255) aCCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGt < 1:3251640/71‑1 (MQ=255) aCCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGt > 1:3019548/1‑71 (MQ=255) cGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTc > 1:744928/1‑71 (MQ=255) gAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCt > 1:251125/1‑71 (MQ=255) aaaaGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGTCCACAGATTGTTGAGGACGTTCttt < 1:694125/71‑1 (MQ=255) aGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTg < 1:2565183/71‑1 (MQ=255) gCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTgc < 1:916125/71‑1 (MQ=255) gCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTgc < 1:3811508/71‑1 (MQ=255) cATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTgcg > 1:2981222/1‑71 (MQ=255) tCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGTCCACAGATTGTTGAGGACGTTCttt < 1:3599104/63‑1 (MQ=255) tCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGcc > 1:591596/1‑70 (MQ=255) tCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCca > 1:3671804/1‑71 (MQ=255) cGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCtttt > 1:218250/1‑63 (MQ=255) ataataCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCg > 1:2293329/1‑70 (MQ=255) aTAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCacac < 1:3381759/68‑1 (MQ=255) aTAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCg < 1:2061262/70‑1 (MQ=255) aaTGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGa > 1:2874163/1‑44 (MQ=255) aTGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACa < 1:3933950/71‑1 (MQ=255) tGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTg > 1:1906246/1‑56 (MQ=255) gCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACaa > 1:2309560/1‑70 (MQ=255) cGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACaaa < 1:1859448/70‑1 (MQ=255) cGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACaaa > 1:1930727/1‑70 (MQ=255) gATACAGCACCCGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACaaaaa > 1:3843166/1‑71 (MQ=255) gATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGt < 1:2169418/45‑1 (MQ=255) aTACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAAt < 1:2982762/71‑1 (MQ=255) cAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAgg > 1:3909611/1‑37 (MQ=255) aGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCttttt > 1:3930550/1‑47 (MQ=255) aGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCttttt > 1:198718/1‑47 (MQ=255) cACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCaa > 1:116428/1‑71 (MQ=255) ccAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAaca < 1:1222376/71‑1 (MQ=255) cagcagCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTgcgc < 1:3394471/46‑1 (MQ=255) cagcagCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCAGACCGAACa < 1:2630294/57‑1 (MQ=255) gcagcGGGGCCGTCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGa < 1:2151065/71‑1 (MQ=255) cagcGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGa > 1:838008/1‑70 (MQ=255) cagcGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGAt < 1:2536519/71‑1 (MQ=255) ggggCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAgg > 1:190002/1‑70 (MQ=255) gccgccCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGAt < 1:3576936/64‑1 (MQ=255) ccgccCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGa < 1:230993/71‑1 (MQ=255) gccCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAAt > 1:184924/1‑49 (MQ=255) cccACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAAc > 1:527498/1‑70 (MQ=255) | CGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAAC > minE/1340972‑1341110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |