| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,347,119 | T→C | 7.7% | G204G (GGT→GGC) | yegH → | fused predicted membrane proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,347,119 | 0 | T | C | 7.7% | 212.0 / 7.5 | 78 | G204G (GGT→GGC) | yegH | fused predicted membrane proteins |
| Reads supporting (aligned to +/- strand): ref base T (37/35); new base C (2/4); total (39/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
GTCACCCGACGATCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATCGAGGCGCTCA > minE/1347049‑1347189 | gTCACCCGACGATCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGt < 1:2595673/71‑1 (MQ=255)gTCACCCGACGATCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGt < 1:621652/71‑1 (MQ=255)gTCACCCGACGATCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGt < 1:2925056/71‑1 (MQ=255) tCACCCGACGATCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGtt < 1:552057/71‑1 (MQ=255) cACCCGACGATCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGtt > 1:3560786/1‑70 (MQ=255) aCCCGACGATCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTc > 1:2338604/1‑71 (MQ=255) ccGACGATCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCg < 1:3866394/70‑1 (MQ=255) cgacgaTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:3186732/1‑71 (MQ=255) cgacgaTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc > 1:3438124/1‑71 (MQ=255) gacgaTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCt > 1:2305353/1‑71 (MQ=255) gacgaTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCt < 1:3913319/71‑1 (MQ=255) acgaTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCtt < 1:1173038/71‑1 (MQ=255) cgaTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTc < 1:502480/71‑1 (MQ=255) aTCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGt < 1:1300383/71‑1 (MQ=255) cGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCg < 1:2102827/62‑1 (MQ=255) gTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCa < 1:2501256/70‑1 (MQ=255) gTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCAt < 1:329443/71‑1 (MQ=255) tATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTc > 1:261382/1‑71 (MQ=255) aTTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTcc > 1:2160373/1‑71 (MQ=255) ctctGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGa > 1:1129357/1‑70 (MQ=255) tctGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTc > 1:23943/1‑59 (MQ=255) tctGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCa < 1:3707386/63‑1 (MQ=255) tctGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCa > 1:386258/1‑63 (MQ=255) tctGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGa > 1:3770570/1‑69 (MQ=255) tctGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGa > 1:2286426/1‑69 (MQ=255) tGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCg < 1:1304697/58‑1 (MQ=255) cTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGt < 1:356861/46‑1 (MQ=255) cTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCt < 1:2572179/71‑1 (MQ=255) cTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGC‑‑AAGGTTTCGGCTTCGTCATTCCGAAAGGCt < 1:657006/69‑1 (MQ=25) ttAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc < 1:2287463/51‑1 (MQ=255) ttAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGc < 1:3797905/51‑1 (MQ=255) aaGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGa > 1:3869031/1‑62 (MQ=255) aaGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTAcc > 1:1252503/1‑71 (MQ=255) ttCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCAt < 1:3560445/53‑1 (MQ=255) ttCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGaa < 1:620814/59‑1 (MQ=255) ccTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCa > 1:2204512/1‑50 (MQ=255) cTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGc > 1:1284481/1‑71 (MQ=255) ttGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAg > 1:3196038/1‑55 (MQ=255) ttGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTAc > 1:1356645/1‑60 (MQ=255) gatgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGcc < 1:3757355/70‑1 (MQ=255) atgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCa < 1:3861128/70‑1 (MQ=255) atgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCa < 1:691659/70‑1 (MQ=255) atgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCAt > 1:2789107/1‑71 (MQ=255) atgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCAt > 1:1146873/1‑71 (MQ=255) atgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTAACTGTACGCTGCCa < 1:829506/70‑1 (MQ=255) tgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGaa > 1:2526837/1‑49 (MQ=255) tgatTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGa < 1:2977026/48‑1 (MQ=255) atTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTgg > 1:2878652/1‑71 (MQ=255) ttGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGaaa > 1:623049/1‑47 (MQ=255) ttGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGaaa > 1:433098/1‑47 (MQ=255) ttGGCTTTAGACTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGaaa > 1:3280768/1‑47 (MQ=255) ggCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTa < 1:1350606/50‑1 (MQ=255) cTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTTGTTtct < 1:3901740/71‑1 (MQ=255) cTTTAGCCTGGTGGCAGAAGGCTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGACATTGGTTtct < 1:1358970/71‑1 (MQ=255) ttAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGaaa > 1:1374977/1‑41 (MQ=255) ttAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTAc > 1:481390/1‑53 (MQ=255) ttAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCg > 1:525411/1‑71 (MQ=255) tAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCgg > 1:2368424/1‑71 (MQ=255) aGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGc > 1:2170769/1‑53 (MQ=255) aGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTg > 1:1810181/1‑55 (MQ=255) aGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGt > 1:2815506/1‑71 (MQ=255) gCCTGGTGGCAGAAGGCTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGtg < 1:1174674/71‑1 (MQ=255) gCCTGGTGGCAGAAGGCTTCGGCTTCGTCATTACGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGtg < 1:3835723/71‑1 (MQ=255) ccTGATCGCCGAAGGCCTGGGCTTCCACATCCCGAAGGGCTACCTGTACGccg > 1:3201494/1‑51 (MQ=255) cTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCt < 1:2602111/40‑1 (MQ=255) tggtggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGtgat > 1:2271364/1‑70 (MQ=255) gtggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTtc < 1:3056754/60‑1 (MQ=255) gtggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTgatgat < 1:3412429/71‑1 (MQ=255) tggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCt < 1:2449305/47‑1 (MQ=255) tggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGGCATTGGTTTCTCGGtgatg < 1:290814/68‑1 (MQ=255) tggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGtgatga < 1:3908761/69‑1 (MQ=255) ggCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATc > 1:3325274/1‑70 (MQ=255) gCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATc > 1:1729369/1‑69 (MQ=255) gCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATCg < 1:3550940/70‑1 (MQ=255) gCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATCGa < 1:3189291/71‑1 (MQ=255) gCAGAAGGCTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATCg < 1:3249429/70‑1 (MQ=255) aGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGc < 1:1900937/42‑1 (MQ=255) gAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATCGAGgc < 1:2505729/71‑1 (MQ=255) aaGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATCGAGgcg > 1:2161285/1‑71 (MQ=255) aaGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATCGAGgcg < 1:2955391/71‑1 (MQ=255) aaGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATCGAGgcg < 1:3860722/71‑1 (MQ=255) aaGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATCGAGgcg > 1:3129736/1‑71 (MQ=255) aGGCTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATCGAGgcg > 1:3735471/1‑70 (MQ=255) ggTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATCGAGGCGCt > 1:3678973/1‑71 (MQ=255) tttCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATCGAGGCGCTCa < 1:1267586/71‑1 (MQ=255) | GTCACCCGACGATCGTTATTCTCTGCTTAAGCTTCCTGTTGATGATTGGCTTTAGCCTGGTGGCAGAAGGTTTCGGCTTCGTCATTCCGAAAGGCTACCTGTACGCTGCCATTGGTTTCTCGGTGATGATCGAGGCGCTCA > minE/1347049‑1347189 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |