| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,370,859 | T→C | 6.7% | intergenic (+116/‑14) | yohK → / → cdd | predicted inner membrane protein/cytidine/deoxycytidine deaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,370,859 | 0 | T | C | 6.7% | 293.0 / 9.4 | 104 | intergenic (+116/‑14) | yohK/cdd | predicted inner membrane protein/cytidine/deoxycytidine deaminase |
| Reads supporting (aligned to +/- strand): ref base T (39/58); new base C (4/3); total (43/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
TAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCT > minE/1370791‑1370926 | taattaatGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTAt < 1:2501191/71‑1 (MQ=255)taattaatGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTAt < 1:2746322/71‑1 (MQ=255)taattaatGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTAt > 1:143100/1‑71 (MQ=255) aattaatGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTAt > 1:81105/1‑70 (MQ=255) attaatGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATg < 1:334099/70‑1 (MQ=255) atGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGc < 1:3583566/70‑1 (MQ=255) atGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGc < 1:1317168/70‑1 (MQ=255) tGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGa < 1:2995922/66‑1 (MQ=255) agaTTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACg > 1:3300635/1‑71 (MQ=255) agaTTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACg > 1:1426207/1‑71 (MQ=255) gaTTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGc < 1:317386/71‑1 (MQ=255) ttCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGcc < 1:1181131/70‑1 (MQ=255) ttCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCa > 1:3784143/1‑71 (MQ=255) cAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATg > 1:10785/1‑71 (MQ=255) aGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATg < 1:152029/70‑1 (MQ=255) aGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGc < 1:1189717/71‑1 (MQ=255) tCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATc < 1:1816825/71‑1 (MQ=255) tCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATc > 1:704160/1‑71 (MQ=255) tCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATc > 1:1122918/1‑71 (MQ=255) cacaTATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATc > 1:215249/1‑70 (MQ=255) atatAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAgg < 1:26645/53‑1 (MQ=255) atatAAAGCCA‑AACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGt < 1:2095069/71‑1 (MQ=255) tataAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAgg > 1:593599/1‑52 (MQ=255) tataAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCGCGt > 1:1953288/1‑71 (MQ=255) ataAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACg > 1:2520542/1‑56 (MQ=255) ataAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGcc < 1:87491/58‑1 (MQ=255) ataAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGtt > 1:1943721/1‑71 (MQ=255) aaaGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAgg < 1:1594945/49‑1 (MQ=255) aaaGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAgg < 1:2330119/49‑1 (MQ=255) aGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTc > 1:1618794/1‑70 (MQ=255) aGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCa < 1:3279408/71‑1 (MQ=255) aGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCa < 1:3296937/71‑1 (MQ=255) aGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCa < 1:3605270/71‑1 (MQ=255) acaACGGGTTCGTAAACTGTTATCCCATTACATGATTATGa > 1:303457/1‑41 (MQ=255) acaACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCAt < 1:1613061/52‑1 (MQ=255) acaACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGTATCCACGTTTTCAAAcc < 1:578236/71‑1 (MQ=255) acaACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAAcc < 1:1988627/71‑1 (MQ=255) caACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGc < 1:2803365/48‑1 (MQ=255) caACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGc < 1:689547/48‑1 (MQ=255) aaCGGGTTCGTAAACTGTTATCCCATTACATGATTa > 1:3512963/1‑36 (MQ=255) aaCGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCaa < 1:3350369/44‑1 (MQ=255) aaCGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGc < 1:1303317/71‑1 (MQ=255) aaCGGGTTCGTAAACTGTTATCCCATTACATGATCATGAGGCAACGCCATGCATCCACGTTTTCa > 1:2229880/1‑65 (MQ=255) aaCGGGTTCGTAAACTGTTATCCCATTACATGATCATGAGGCAACGCCATGCATCCACGTTTTCa > 1:3649683/1‑65 (MQ=255) aCGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCAt > 1:3748363/1‑49 (MQ=255) aCGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGc < 1:1459726/70‑1 (MQ=255) cGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCtt > 1:3918730/1‑71 (MQ=255) cGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCtt < 1:2132094/71‑1 (MQ=255) gggTTCGTAAACTGTTATCCCATTACATGATTATTAGGCAACGCCATGCATCCACGTTTTCAAACCGCttt < 1:2006276/71‑1 (MQ=255) gggTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGc < 1:68363/44‑1 (MQ=255) gggTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCtt < 1:803736/70‑1 (MQ=255) ggTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCtttt < 1:264524/71‑1 (MQ=255) ggTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCtttt < 1:3735249/71‑1 (MQ=255) gTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTg < 1:972134/71‑1 (MQ=255) tCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCaaa > 1:1606603/1‑60 (MQ=255) cGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGtttt > 1:3108275/1‑55 (MQ=255) cGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGcc > 1:3430834/1‑70 (MQ=255) cGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGcc > 1:3105297/1‑70 (MQ=255) tAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGc > 1:3106378/1‑42 (MQ=255) tAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGc < 1:81305/42‑1 (MQ=255) tAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCaa > 1:770377/1‑71 (MQ=255) tAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCaa < 1:1231122/71‑1 (MQ=255) aaCTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGttt < 1:483338/50‑1 (MQ=255) aaCTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACt > 1:2788784/1‑71 (MQ=255) aCTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACtt < 1:811517/71‑1 (MQ=255) aCTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACtt < 1:2660740/71‑1 (MQ=255) aCTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACtt < 1:2080647/71‑1 (MQ=255) aCTGTTATCCCATTACATGATCATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACtt < 1:2413661/71‑1 (MQ=255) cTGTTATCCCATTACATGATCATGAGGCAACGCCATGCATCCa > 1:3127054/1‑43 (MQ=255) cTGTTATCCCATAACATGATCATGAGGCAACGCCATGCATCCACGATTTCAAACCGCTATTGCCCAACTTg > 1:3838197/1‑71 (MQ=255) tGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGtt < 1:1053505/46‑1 (MQ=255) tGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGtt < 1:495659/46‑1 (MQ=255) gTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGATTTTGc < 1:512942/61‑1 (MQ=255) ttATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGc < 1:3319171/69‑1 (MQ=255) ttATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCg < 1:1623341/70‑1 (MQ=255) ttATCCCATTACATGATTATGAGGCAACCCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCgg < 1:3904087/71‑1 (MQ=255) tCCCATTACATGATTATGAGGCAACGCCATGCATCCACGttt < 1:2022629/42‑1 (MQ=255) tCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGAt < 1:1459177/70‑1 (MQ=255) ccATTACATGATTATGAGGCAACGCCATGCATCCAc > 1:3952541/1‑36 (MQ=255) ccATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACtt < 1:1124580/62‑1 (MQ=255) ccATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAAc < 1:3773826/71‑1 (MQ=255) ccATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAAc > 1:2760523/1‑71 (MQ=255) cATTACATGATTATGAGGCAACGTCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACt > 1:31761/1‑71 (MQ=255) cATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACt > 1:3509086/1‑71 (MQ=255) cATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACt > 1:2956425/1‑71 (MQ=255) cATTACATGATCATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACt < 1:711767/71‑1 (MQ=255) ttACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTg > 1:33375/1‑71 (MQ=255) ttACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTg < 1:1492510/71‑1 (MQ=255) ttACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTg > 1:2080999/1‑71 (MQ=255) aCATGATCATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGc < 1:1822022/70‑1 (MQ=255) cATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCaa < 1:692356/71‑1 (MQ=255) aTGATTATGAGGCAACGCCATGCATCCACGTTTTCa < 1:668470/36‑1 (MQ=255) aTGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAAt > 1:2290867/1‑71 (MQ=255) aTGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAAt < 1:1642469/71‑1 (MQ=255) aTGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAAt < 1:1915914/71‑1 (MQ=255) tGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAAt < 1:2442651/70‑1 (MQ=255) tGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATc > 1:1136871/1‑71 (MQ=255) tGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATc < 1:3467809/71‑1 (MQ=255) gATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCt > 1:2965457/1‑71 (MQ=255) ttATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTg > 1:1784000/1‑63 (MQ=255) | TAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCT > minE/1370791‑1370926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |