| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,374,127 | T→C | 18.0% | L356L (TTA→CTA) | yeiT → | predicted oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,374,127 | 0 | T | C | 18.0% | 103.9 / 17.8 | 50 | L356L (TTA→CTA) | yeiT | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (17/24); new base C (6/3); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01 | |||||||||||
ACTGACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTTG > minE/1374057‑1374190 | aCTGACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGt > 1:3747464/1‑71 (MQ=255) tGACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGt > 1:2931640/1‑69 (MQ=255) tGACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGt > 1:3245883/1‑69 (MQ=255) tGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGtt > 1:156724/1‑64 (MQ=255) gcAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTa > 1:2951310/1‑60 (MQ=255) gcAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTa > 1:3069519/1‑60 (MQ=255) aTAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTaa > 1:3938533/1‑70 (MQ=255) aaTTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGt < 1:2972206/64‑1 (MQ=255) ttattCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATc < 1:581516/71‑1 (MQ=255) ttattCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTaga < 1:3665173/52‑1 (MQ=255) attCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGc > 1:3770793/1‑52 (MQ=255) ttCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCaaa < 1:557043/71‑1 (MQ=255) tctcGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaa < 1:713656/69‑1 (MQ=255) ctcGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAg < 1:2333246/54‑1 (MQ=255) ctcGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAg < 1:1820646/54‑1 (MQ=255) ctcGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaaaa < 1:3418865/70‑1 (MQ=255) ctcGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaaaa < 1:130424/70‑1 (MQ=255) ctcGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCaaaa < 1:3911499/70‑1 (MQ=255) gCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTaa > 1:2916895/1‑56 (MQ=255) gCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTag > 1:2097826/1‑43 (MQ=255) gCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAaca > 1:3491080/1‑69 (MQ=255) cGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaaa < 1:1693615/64‑1 (MQ=255) gTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACaaaa > 1:1238988/1‑71 (MQ=255) gTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACaaaa > 1:690421/1‑71 (MQ=255) gTCGGTCAGCATGCCAGACTAGATGCCATTGCGGAGCTAGAGCCGCAGCGTAacac > 1:77517/1‑56 (MQ=255) ggTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTag < 1:3732709/37‑1 (MQ=255) ggTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTag > 1:3530181/1‑37 (MQ=255) ggTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTag < 1:903254/37‑1 (MQ=255) ggTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTaa > 1:2590372/1‑50 (MQ=255) ggTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTaa > 1:2451255/1‑50 (MQ=255) ggTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAAtt < 1:2596899/70‑1 (MQ=255) ggTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAAtt < 1:69908/70‑1 (MQ=255) tCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAAt < 1:3760429/67‑1 (MQ=255) aGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAc > 1:2557641/1‑68 (MQ=255) aGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCa < 1:246633/70‑1 (MQ=255) aGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCa < 1:3692646/70‑1 (MQ=255) tGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTaa > 1:1546004/1‑42 (MQ=255) tGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTaa > 1:431973/1‑42 (MQ=255) tGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTaa > 1:412468/1‑42 (MQ=255) ccAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgc > 1:2004562/1‑71 (MQ=255) ccAGACTAGATGCCTTTGCGGAGCTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgc > 1:3471961/1‑71 (MQ=255) cAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGc < 1:987663/35‑1 (MQ=255) cAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcg < 1:185101/71‑1 (MQ=255) cAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcg < 1:3477118/71‑1 (MQ=255) gACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcgac > 1:1969249/1‑71 (MQ=255) aCTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcgacc < 1:1647169/71‑1 (MQ=255) cTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAcc < 1:565123/58‑1 (MQ=255) cTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTAcc < 1:2402895/58‑1 (MQ=255) gATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCaaa < 1:3915298/42‑1 (MQ=255) gATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAgacccgcgacccgc < 1:831920/70‑1 (MQ=255) cTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCt < 1:1353093/71‑1 (MQ=255) tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAac > 1:1557596/1‑36 (MQ=255) tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAac > 1:1070442/1‑36 (MQ=255) tGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTtg > 1:1634245/1‑71 (MQ=255) | ACTGACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCATGCCAGACTAGATGCCTTTGCGGAGTTAGAGCCGCAGCGTAACACCATCAAAACACAAAATTACCAGACCCGCGACCCGCAAGTCTTTG > minE/1374057‑1374190 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |