| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,390,615 | A→G | 57.0% | Q249Q (CAA→CAG) | nfo → | endonuclease IV with intrinsic 3'‑5' exonuclease activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,390,615 | 0 | A | G | 57.0% | 36.4 / 102.4 | 86 | Q249Q (CAA→CAG) | nfo | endonuclease IV with intrinsic 3'‑5' exonuclease activity |
| Reads supporting (aligned to +/- strand): ref base A (17/20); new base G (26/23); total (43/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-01 | |||||||||||
GCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAG > minE/1390545‑1390681 | gCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCaa < 1:2931334/71‑1 (MQ=255) gcgTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAga > 1:3066116/1‑70 (MQ=255) gTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAgacga > 1:3275348/1‑71 (MQ=255) tGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGAcc > 1:3768036/1‑71 (MQ=255) aCCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGt < 1:3028768/71‑1 (MQ=255) cGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAgacg > 1:2721176/1‑64 (MQ=255) gCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGgacga > 1:779883/1‑64 (MQ=255) gCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTc < 1:2183682/71‑1 (MQ=255) ccATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTc > 1:1901954/1‑70 (MQ=255) ccATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCg < 1:2366201/71‑1 (MQ=255) ccATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCg < 1:2197719/71‑1 (MQ=255) ccATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCg < 1:1491157/71‑1 (MQ=255) catcatAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGa > 1:2640918/1‑71 (MQ=255) catcatAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGa > 1:3892565/1‑71 (MQ=255) atcatATCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGAc > 1:1220679/1‑71 (MQ=255) tcatAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGgacg < 1:1155640/59‑1 (MQ=255) tcatAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAga > 1:1804050/1‑57 (MQ=255) tcatAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGa > 1:2094272/1‑69 (MQ=255) catAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACgg > 1:1407377/1‑71 (MQ=255) catAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACgg > 1:1402780/1‑71 (MQ=255) catAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGt < 1:3063296/63‑1 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGgac < 1:2014106/56‑1 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACgg < 1:3532548/70‑1 (MQ=255) atAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACgg < 1:3938568/70‑1 (MQ=255) tAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCa < 1:2935888/71‑1 (MQ=255) tAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCa < 1:2503202/71‑1 (MQ=255) tAGCCTCGGTGAAGGCAATATCGGGCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCa < 1:546053/71‑1 (MQ=255) aGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACgg > 1:1188023/1‑68 (MQ=255) gCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCAtt > 1:1620671/1‑71 (MQ=255) tGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGa < 1:85450/71‑1 (MQ=255) tGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGa < 1:3064890/71‑1 (MQ=255) gAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTcc < 1:3624652/65‑1 (MQ=255) aaGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTg < 1:3884937/68‑1 (MQ=255) aaGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATc < 1:130028/71‑1 (MQ=255) gCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTc < 1:729966/71‑1 (MQ=255) aaTATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGa < 1:1674208/71‑1 (MQ=255) aaTATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGa < 1:2757892/71‑1 (MQ=255) aaTATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGa < 1:2213882/71‑1 (MQ=255) atatCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGAc < 1:1793285/50‑1 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaaa > 1:3742567/1‑71 (MQ=255) tatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaaa > 1:44019/1‑71 (MQ=255) cGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGAt > 1:363270/1‑60 (MQ=255) cGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGt < 1:1491358/40‑1 (MQ=255) ggTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGatcatc > 1:3751276/1‑63 (MQ=255) ggTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTc > 1:1109611/1‑63 (MQ=255) ggTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCa > 1:3495097/1‑70 (MQ=255) ggTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCAt > 1:1408344/1‑71 (MQ=255) gTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCa > 1:2847678/1‑69 (MQ=255) tCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCa < 1:1662084/71‑1 (MQ=255) tCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCa < 1:474266/71‑1 (MQ=255) atgatgCGTTCCGCTGGATCATGCAGGACGACCGTTTCg < 1:2462096/39‑1 (MQ=255) atgatgCGTTCCGCTGGATCATGCAGGACGACCGTTTCg < 1:541017/39‑1 (MQ=255) atgatgCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCa < 1:3504231/66‑1 (MQ=255) atgatgCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAAc > 1:3488214/1‑71 (MQ=255) atgatgCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAAc > 1:83233/1‑71 (MQ=255) tgatgCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAAcc < 1:2562674/71‑1 (MQ=255) gatgCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACg > 1:1163798/1‑40 (MQ=255) atgCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCAt < 1:1173976/43‑1 (MQ=255) tgCGTTCCGCTGGATCATGCAAGACGCCCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg > 1:3125333/1‑70 (MQ=255) tgCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg > 1:1216162/1‑70 (MQ=255) tgCGTTCCGCTGGATCATGCAAGACGACAGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg > 1:2500734/1‑70 (MQ=255) tgccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATc < 1:95186/58‑1 (MQ=255) tCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATc > 1:1733302/1‑71 (MQ=255) ccGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCt > 1:1982138/1‑71 (MQ=255) ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAAccc > 1:1706866/1‑63 (MQ=255) ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAAccc > 1:3513282/1‑63 (MQ=255) cGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCt < 1:1934487/70‑1 (MQ=255) cGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCt < 1:1447086/70‑1 (MQ=255) cGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACAATCAACCCGGATATCt < 1:772766/70‑1 (MQ=255) cGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTg < 1:2465697/71‑1 (MQ=255) gATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGc < 1:3353529/69‑1 (MQ=255) gATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGa < 1:1411147/45‑1 (MQ=255) gATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGa < 1:813857/45‑1 (MQ=255) gATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGa < 1:3946063/45‑1 (MQ=255) gATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTggg > 1:3046799/1‑68 (MQ=255) aTCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCt > 1:2346471/1‑64 (MQ=255) tCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATc > 1:3393154/1‑50 (MQ=255) tCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATc > 1:2330021/1‑50 (MQ=255) tCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaa < 1:1927253/71‑1 (MQ=255) aTGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaa > 1:3304392/1‑69 (MQ=255) tGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:821385/71‑1 (MQ=255) tGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:530208/71‑1 (MQ=255) gCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAAc > 1:3311206/1‑49 (MQ=255) gCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAAc > 1:1662436/1‑49 (MQ=255) gCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaa > 1:1993363/1‑67 (MQ=255) gCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCagaa > 1:3155964/1‑67 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAAccc > 1:899921/1‑50 (MQ=255) | GCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAG > minE/1390545‑1390681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |