| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,395,087 | T→C | 10.4% | intergenic (‑160/‑208) | fruB ← / → setB | fused fructose‑specific PTS enzyme IIA component and HPr component/lactose/glucose efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,395,087 | 0 | T | C | 10.4% | 202.3 / 12.9 | 77 | intergenic (‑160/‑208) | fruB/setB | fused fructose‑specific PTS enzyme IIA component and HPr component/lactose/glucose efflux system |
| Reads supporting (aligned to +/- strand): ref base T (30/39); new base C (3/5); total (33/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
AACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAATTTC > minE/1395019‑1395156 | aaCGTTTCAAGAAAGCATAATACTTCTTTTTCAGAACTCAAGGAAAGCCGTAAAGTTATTAGCAAAAGt < 1:1382931/69‑1 (MQ=255)aaCGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGttt < 1:1419358/71‑1 (MQ=255)aaCGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGttt < 1:711577/71‑1 (MQ=255)aaCGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGt > 1:3289577/1‑69 (MQ=255)aaCGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGctt > 1:2428467/1‑71 (MQ=255) cGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGa > 1:1607243/1‑71 (MQ=255) tttCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGAtg < 1:2750746/71‑1 (MQ=255) tttCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGAtg < 1:1232199/71‑1 (MQ=255) tCAAGAAAGGATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGAtgt < 1:3794849/70‑1 (MQ=255) tCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGAtgt < 1:122931/70‑1 (MQ=255) cAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGAtgtg < 1:2710319/70‑1 (MQ=255) cAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGAtgtg < 1:173493/70‑1 (MQ=255) cAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGAtgtg < 1:1957565/70‑1 (MQ=255) aaaGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGca > 1:571165/1‑71 (MQ=255) aaaGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGca > 1:1746735/1‑71 (MQ=255) aaGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGca > 1:2213229/1‑70 (MQ=255) aGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGcaca < 1:370921/71‑1 (MQ=255) gCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGtgctg < 1:1746903/66‑1 (MQ=255) gCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGtgctg < 1:466081/66‑1 (MQ=255) gCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACaa < 1:2745000/71‑1 (MQ=255) ataataCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGttt < 1:1525139/55‑1 (MQ=255) taataCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAAttt < 1:909375/71‑1 (MQ=255) ataCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAAttttt < 1:3029451/71‑1 (MQ=255) ataCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAAtttt < 1:516555/70‑1 (MQ=255) ataCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAAtttt < 1:2944210/70‑1 (MQ=255) ataCTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAAtttt < 1:2163676/70‑1 (MQ=255) cTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAt > 1:3686559/1‑71 (MQ=255) cTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAt > 1:2053216/1‑71 (MQ=255) cTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAt > 1:2852826/1‑71 (MQ=255) tttCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGtt < 1:2507216/42‑1 (MQ=255) tttCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAtttt < 1:388545/71‑1 (MQ=255) tttCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAtttt < 1:2253915/71‑1 (MQ=255) tttCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAttt < 1:864593/70‑1 (MQ=255) tttCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAttt < 1:3459800/70‑1 (MQ=255) tttCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAt < 1:2546294/68‑1 (MQ=255) tttCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCAtttt < 1:207532/71‑1 (MQ=255) tCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGt > 1:1940913/1‑39 (MQ=255) tCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGt > 1:2169696/1‑39 (MQ=255) tCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAttttt > 1:2261095/1‑70 (MQ=255) aGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAttttt > 1:2200991/1‑68 (MQ=255) aGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAttttt > 1:3487482/1‑68 (MQ=255) aGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCt < 1:3313871/71‑1 (MQ=255) aGCACGAAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTg < 1:2690718/40‑1 (MQ=255) aCGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGAt > 1:1284425/1‑39 (MQ=255) aCGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAAt < 1:3370218/71‑1 (MQ=255) gCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTg < 1:2642057/71‑1 (MQ=255) gCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTg < 1:489728/71‑1 (MQ=255) gCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTa < 1:1207018/67‑1 (MQ=255) gCAAGGAAAGCCGTAAAGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTa < 1:1667474/67‑1 (MQ=255) aaGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCt > 1:355761/1‑71 (MQ=255) aaGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCt > 1:3619652/1‑71 (MQ=255) aaGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCt > 1:1083969/1‑71 (MQ=255) ggAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGa < 1:2359837/71‑1 (MQ=255) ggAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGa < 1:1263380/71‑1 (MQ=255) ggAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGa < 1:3896336/71‑1 (MQ=255) ggAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGa < 1:955257/71‑1 (MQ=255) ggAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGa < 1:3261951/71‑1 (MQ=255) gAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAttttt > 1:3408676/1‑58 (MQ=255) aGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATgg > 1:290561/1‑70 (MQ=255) gCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGa < 1:1767352/71‑1 (MQ=255) cGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAAttt < 1:2278554/40‑1 (MQ=255) tAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTg > 1:2076586/1‑61 (MQ=255) tAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTg > 1:3629012/1‑61 (MQ=255) tAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAAt < 1:3557707/71‑1 (MQ=255) aaaGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATc > 1:1788772/1‑71 (MQ=255) aaaGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATc > 1:2155804/1‑71 (MQ=255) aaGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAtttt > 1:3223986/1‑47 (MQ=255) aaGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAtttt > 1:3219307/1‑47 (MQ=255) aaGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATgg > 1:2672995/1‑63 (MQ=255) aaGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATcc > 1:714642/1‑71 (MQ=255) aaGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATcc > 1:2966481/1‑71 (MQ=255) aaGTTATGAGCAAAAGCTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATcc > 1:3118790/1‑71 (MQ=255) ttATGAGCTAAAGCTTGATGTGCTGCACAATTTTTTGCCAttttt < 1:188187/45‑1 (MQ=38) aTGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATcc > 1:2533812/1‑66 (MQ=255) gAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTa > 1:3638579/1‑45 (MQ=255) aGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCa < 1:1029737/70‑1 (MQ=255) gCAAAAGTTTGATGTGCTGCACAATTTTTTGCCAttttt > 1:2668680/1‑39 (MQ=255) aaaaGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAAtt > 1:1254254/1‑44 (MQ=255) aaaaGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGc < 1:1522185/46‑1 (MQ=255) aaaGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAat > 1:2885633/1‑71 (MQ=255) aaaGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAat > 1:2124387/1‑71 (MQ=255) aaaGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAat < 1:2395465/71‑1 (MQ=255) aaGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAatt > 1:1474229/1‑71 (MQ=255) aaGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAatt < 1:2512450/71‑1 (MQ=255) aGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAattt < 1:988017/71‑1 (MQ=255) gTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAatttc < 1:2516100/71‑1 (MQ=255) | AACGTTTCAAGAAAGCATAATACTTCTGTTTCAGCACGCAAGGAAAGCCGTAAAGTTATGAGCAAAAGTTTGATGTGCTGCACAATTTTTTGCCATTTTTCCTAATTGCTGATGGGAAAATCCGTTATCAGCAATTTC > minE/1395019‑1395156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |