| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,395,393 | G→T | 11.2% | L33L (CTG→CTT) | setB → | lactose/glucose efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,395,393 | 0 | G | T | 11.2% | 221.1 / 9.2 | 71 | L33L (CTG→CTT) | setB | lactose/glucose efflux system |
| Reads supporting (aligned to +/- strand): ref base G (29/34); new base T (4/4); total (33/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01 | |||||||||||
AAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTC > minE/1395325‑1395462 | aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGc > 1:980850/1‑70 (MQ=255) aaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCaa < 1:648864/71‑1 (MQ=255) aaTCGTTTGAACTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCa > 1:1825302/1‑70 (MQ=255) tCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAc > 1:3296002/1‑71 (MQ=255) gTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAccc > 1:2012424/1‑71 (MQ=255) gTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAccc > 1:2887438/1‑71 (MQ=255) tgacctgaccTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGa > 1:112253/1‑71 (MQ=255) cctgacctCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGacac > 1:3348220/1‑71 (MQ=255) acctCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAg < 1:2421926/71‑1 (MQ=255) cctCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAccc > 1:3802522/1‑60 (MQ=255) cctCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAg > 1:1699721/1‑70 (MQ=255) tCGACGGCGTTTTCAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGa < 1:178586/61‑1 (MQ=255) gACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTAtt < 1:213680/70‑1 (MQ=255) cGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTAtattc > 1:491273/1‑70 (MQ=255) ggCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTAtattcc < 1:666666/71‑3 (MQ=255) gcatttttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGt > 1:3578279/4‑63 (MQ=255) gTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTa < 1:2077634/71‑1 (MQ=255) gTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTa > 1:182198/1‑71 (MQ=255) gTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTa > 1:704505/1‑71 (MQ=255) tttttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCACGACACTCAGTATTTTTCTTa < 1:2188535/70‑1 (MQ=255) ttttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCtcg > 1:3049004/1‑67 (MQ=255) tttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCaa < 1:984101/43‑1 (MQ=255) tttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCaa < 1:1047866/43‑1 (MQ=255) ttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGac > 1:1698816/1‑50 (MQ=255) aaTCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGa > 1:350033/1‑47 (MQ=255) aaTCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGa > 1:3798978/1‑47 (MQ=255) aaTCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGa > 1:2647527/1‑47 (MQ=255) ttGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTAc < 1:1059526/61‑1 (MQ=255) ttGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGaa > 1:1456976/1‑68 (MQ=255) tGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTa > 1:2385743/1‑70 (MQ=255) gCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAcccc < 1:3530642/38‑1 (MQ=255) gCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTAc > 1:1693384/1‑70 (MQ=255) ccTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACAt < 1:2230401/71‑1 (MQ=255) cTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATg < 1:3904721/71‑1 (MQ=255) tttCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGa < 1:2382104/37‑1 (MQ=255) tttCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGa < 1:2548102/37‑1 (MQ=255) ccGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTa > 1:2163567/1‑59 (MQ=255) ccGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTa > 1:1866052/1‑59 (MQ=255) gggTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTccta < 1:1933850/70‑3 (MQ=255) gggTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTc < 1:1432052/67‑1 (MQ=255) cGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTAc > 1:1954311/1‑59 (MQ=255) ggTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTcctat < 1:3361635/71‑4 (MQ=255) tATTGCGGGCGCTCAGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTa < 1:3766127/55‑1 (MQ=255) ttGCGGGCGCTCTTCAGACTCCTACCCTAAGTAta > 1:1284793/1‑34 (MQ=255) ttGCGGGCGCTCTGCAAACCCCGACACTCAGTAttttt < 1:2068429/38‑1 (MQ=255) ttGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTc < 1:2622723/39‑1 (MQ=255) ttGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtg < 1:214826/71‑1 (MQ=255) ttGCGGGCGCTCTGCAAACCACGACACTCAGTATTTTTCTTACCGATg < 1:3534199/48‑1 (MQ=255) gCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTc > 1:1289580/1‑61 (MQ=255) gCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtgg < 1:3050815/70‑1 (MQ=255) gCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtgg < 1:118666/70‑1 (MQ=255) ggCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGaa < 1:3137898/45‑1 (MQ=255) ggCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGAtggtgg < 1:2748935/70‑1 (MQ=255) gcgcTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTcc < 1:1331092/58‑1 (MQ=255) cgcTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAt > 1:3148345/1‑71 (MQ=255) ctctGCAAACCCCGACACTCAGTATTTTTCTTACCGa < 1:1116466/37‑1 (MQ=255) ctctGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttc > 1:3719713/1‑71 (MQ=255) ctctGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttc < 1:2890552/71‑1 (MQ=255) ctctGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttc < 1:2050547/71‑1 (MQ=255) ctctGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttc < 1:2458615/71‑1 (MQ=255) ctctGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttc < 1:23755/71‑1 (MQ=255) tctGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGc > 1:2068475/1‑48 (MQ=255) tctGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGa < 1:1563046/67‑1 (MQ=255) tctGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGa < 1:2726225/67‑1 (MQ=255) tctGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttct > 1:1808239/1‑71 (MQ=255) ctGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACAt > 1:239995/1‑45 (MQ=255) tGCAAACCCCGACACTCAGTATTTTTCTTACCGATg < 1:2726315/36‑1 (MQ=255) tGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGt > 1:1391204/1‑40 (MQ=255) tGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttcttc > 1:3081368/1‑71 (MQ=255) tGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttct < 1:453422/69‑1 (MQ=255) tGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGAttct < 1:2067071/69‑1 (MQ=255) | AAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTCCGGCGATGGTGGGATTCTTC > minE/1395325‑1395462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |